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Water deficit and exogenous ABA significantly affect grape and wine phenolic composition under in field and in-vitro conditions

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Abstract

Phenolic compounds are components responsible of the sensorial characteristics of red wine. Indeed, they present important properties for human health. In the present work, the effects of combined in field treatments (water-stress and exogenous ABA) on phenolic accumulation were evaluated for berries and wine. The responses were assessed by UV–Vis and CZE. A differential phenolic regulation was observed under the same conditions and good correlations were achieved for grape and wine. ABA appears to regulate the content of each phenols under study depending on plant water-status. Although the effects of water-stress and exogenous ABA were similar in magnitude, our results support evidence suggesting that both affect different metabolic pathways. ABA supply increased catechin and malvidin synthesis for both water statuses while the resveratrol was enhanced only for water-stress. Indeed, ABA reduced the quercetin content for both water statuses. In vitro assays were carried out to estimate the effects of combined treatments (temperature-exogenous ABA) on anthocyanin accumulation in berries and pulp. These tests demonstrated that the effect of ABA was dependent on the hormonal level (207% for 0.5 g L−1 and 307% for 1 g L−1 of ABA). Effect of temperature varied according to the phenological state. In pulps, we detected an increase of anthocyanin after ABA treatments.

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Abbreviations

BGE:

Background electrolyte

OD:

Outer diameter

CE:

Capillary electrophoresis

CZE:

Capillary zone electrophoresis

EOF:

Electroosmotic flow

HPLC:

High performance liquid chromatography

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Acknowledgments

This work was supported by funding from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Cuyo (Argentina) (Projects 06/A354 and 06/PA07), B. Cavagnaro, R. Bottini, R. Wuilloud and M.F. Silva.

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Authors and Affiliations

  1. Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Alte. Brown 500, 5507, Chacras de Coria, Mendoza, Argentina

    Leonor Deis, Bruno Cavagnaro, Ruben Bottini & M. Fernanda Silva

  2. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Mendoza, Argentina

    Leonor Deis, Bruno Cavagnaro, Ruben Bottini, Rodolfo Wuilloud & M. Fernanda Silva

  3. Laboratorio de Investigaciones y Servicios Ambientales Mendoza (LISAMEN), CRICYT—CONICET, Av. Ruiz Leal s/n, Parque General San Martín, 5500, Mendoza, Argentina

    Rodolfo Wuilloud

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  1. Leonor Deis
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  2. Bruno Cavagnaro
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  3. Ruben Bottini
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  4. Rodolfo Wuilloud
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  5. M. Fernanda Silva
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Correspondence to Leonor Deis.

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Deis, L., Cavagnaro, B., Bottini, R. et al. Water deficit and exogenous ABA significantly affect grape and wine phenolic composition under in field and in-vitro conditions. Plant Growth Regul 65, 11–21 (2011). https://doi.org/10.1007/s10725-011-9570-5

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  • DOI: https://doi.org/10.1007/s10725-011-9570-5

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