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Tobacco plants ectopically expressing the Ammopiptanthus mongolicus AmCBL1 gene display enhanced tolerance to multiple abiotic stresses

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Abstract

Ca2+ is a critical component in signal transduction pathways that lead to stress gene expression in higher plants and regulates a wide range of physiological processes. The calcineurin B-like protein (CBL) family represents a unique group of calcium sensors in plants, and plays a key role in decoding calcium transients. Here, a CBL1 homolog gene, AmCBL1 (NCBI accession no. AY902246), was isolated from Ammopiptanthus mongolicus (Maxim.) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Quantitative real-time PCR (QRT-PCR) experiments showed that AmCBL1 was significantly induced by drought, high salinity, heat, and CaCl2 treatments in A. mongolicus seedlings. Subcellular localization analysis suggested that AmCBL1 is a plasma membrane-localized protein. Our results suggest that AmCBL1 is not only a positive regulator of salt response but also a positive regulator of temperature stresses in A. mongolicus.

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Abbreviations

ABA:

Abscisic acid

CaM:

Calmodulin

CAML:

Calmodulin like proteins

CBL:

Calcineurin B-like

CDPK:

Calcium dependent protein kinase

CIPK:

CBL-interacting protein kinases

CNB:

Calcineurin B

GFP:

Green fluorescent protein

NCS:

Neuronal calcium sensors

ORF:

Open reading frame

PCR:

Polymerase chain reaction

qRT-PCR:

Quantitative real-time PCR

RACE:

Rapid amplification cDNA ends

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Acknowledgments

This work was supported by the Hi-Tech Research and Development Program of China (2007AA10Z106), National Natural Science Foundation of China (30730077, 30972339, 31070597), Program for New Century Excellent Talents in University of China (NCET-07-0083), “948” Project of State Forestry Administration of China (2007-4-01), and the Fundamental Research funds for central universities (Y×2010-17).

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China

    Jin-Huan Chen, Yan Sun, Fang Sun, Xin-Li Xia & Wei-Lun Yin

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  1. Jin-Huan Chen
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  2. Yan Sun
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  3. Fang Sun
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  4. Xin-Li Xia
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  5. Wei-Lun Yin
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Correspondence to Xin-Li Xia or Wei-Lun Yin.

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Chen, JH., Sun, Y., Sun, F. et al. Tobacco plants ectopically expressing the Ammopiptanthus mongolicus AmCBL1 gene display enhanced tolerance to multiple abiotic stresses. Plant Growth Regul 63, 259–269 (2011). https://doi.org/10.1007/s10725-010-9523-4

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