Abstract
The effects of sodium nitroprusside (SNP) on callus induction and shoot regeneration of Dioscorea opposite Thunb. have been studied. Application of 40 μM of SNP depresses accumulation of H2O2 in tuber explants of Dioscorea opposita markedly. Supplementation of 40 μM of SNP to the Murashige and Skoog medium with combinations of benzylaminopurine (3 mg dm−3) and naphthaleneacetic acid (0.5 mg dm−3) reduces the browning of explants and increases the frequency of callus induction from tuber explants significantly. The regeneration frequency of adventitious shoot shows a significant increase in the presence of SNP. Further analysis indicates that treatment with 40 μM of SNP results in significant decreases in catalase and peroxidase activity, while increasing the activity of superoxide dismutase. Supplementation with 40 μM of SNP also promotes the accumulation of non-enzymic antioxidants, including proline and glutathione. The effects on callus induction and shoot regeneration promoted by SNP were reversed by the nitric oxide (NO) scavenger 2-(4-carboxyphenyl)- 4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide. These results indicate that the exogenously applied NO-donor SNP alleviates browning of tuber explants by reducing H2O2 accumulation, thereby promoting a higher in vitro proliferation frequency of D. opposita.
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The research was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant no. 0707013603).
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Xu, J., Yin, H., Wang, W. et al. Effects of sodium nitroprusside on callus induction and shoot regeneration in micropropagated Dioscorea opposita . Plant Growth Regul 59, 279–285 (2009). https://doi.org/10.1007/s10725-009-9410-z
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DOI: https://doi.org/10.1007/s10725-009-9410-z