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Identification of stripe rust resistant genes and their validation in seedling and adult plant glass house tests

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Abstract

Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is considered as a major threat to the wheat production worldwide, including Pakistan. In the current study, 80 wheat landraces collected from different agroecological zones of Pakistan were screened for their stripe rust resistance. Molecular markers were used to identify/postulate the stripe rust genes in these landraces. The results indicated that 46 (57.5%) landraces had all-stage resistance, and only 4 (5%) landraces showed adult-plant resistance. The possible stripe rust resistance genes in these entries were postulated by the using already reported linked molecular markers of all-stage resistance and adult plant stage resistance genes (Yr4 Yr5, Yr10, Yr15 and Yr24/26 and adult-plant resistance genes Yr18, Yr29 and Yr32). Molecular analysis indicated that resistant genes Yr4, Yr5, Yr10, Yr15, Yr17, Yr18, Yr26/24, Yr29 and Yr32 were found in 16(20%), 12(15%), 4(5%), 15(18.75%), 16(20%), 13(16.25%), 39(48.75%), 20(25%) and 7(7.5%) of landraces, respectively. Majority of the landraces showed the presence of more than one gene. These lines could be used effectively in the wheat breeding programs.

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Acknowledgements

This research work was supported by COMSATS Research Grant Program (CRGP, Project #604). Dr. Aisha Khan, Assistant Professor, Department of Humanities, COMSATS University Islamabad.

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Authors and Affiliations

  1. Department of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan

    Shamaila Tahir, Iram Zia, Iqra Dilshad, Nighat Noureen & Sumaira Farrakh

  2. National Agricultural Research Center, Islamabad, Pakistan

    Muhammad Fayyaz

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  1. Shamaila Tahir
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  2. Iram Zia
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  4. Muhammad Fayyaz
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Correspondence to Sumaira Farrakh.

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Tahir, S., Zia, I., Dilshad, I. et al. Identification of stripe rust resistant genes and their validation in seedling and adult plant glass house tests. Genet Resour Crop Evol 67, 1025–1036 (2020). https://doi.org/10.1007/s10722-020-00898-4

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