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Genetic mapping of stem rust resistance gene Sr13 in tetraploid wheat (Triticum turgidum ssp. durum L.)

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A Comment to this article was published on 14 December 2010

Abstract

Wheat stem rust caused by Puccinia graminis f. sp. tritici, can cause significant yield losses. To combat the disease, breeders have deployed resistance genes both individually and in combinations to increase resistance durability. A new race, TTKSK (Ug99), identified in Uganda in 1999 is virulent on most of the resistance genes currently deployed, and is rapidly spreading to other regions of the world. It is therefore important to identify, map, and deploy resistance genes that are still effective against TTKSK. One of these resistance genes, Sr13, was previously assigned to the long arm of chromosome 6A, but its precise map location was not known. In this study, the genome location of Sr13 was determined in four tetraploid wheat (T. turgidum ssp. durum) mapping populations involving the TTKSK resistant varieties Kronos, Kofa, Medora and Sceptre. Our results showed that resistance was linked to common molecular markers in all four populations, suggesting that these durum lines carry the same resistance gene. Based on its chromosome location and infection types against different races of stem rust, this gene is postulated to be Sr13. Sr13 was mapped within a 1.2–2.8 cM interval (depending on the mapping population) between EST markers CD926040 and BE471213, which corresponds to a 285-kb region in rice chromosome 2, and a 3.1-Mb region in Brachypodium chromosome 3. These maps will be the foundation for developing high-density maps, identifying diagnostic markers, and positional cloning of Sr13.

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Acknowledgments

This project was supported in part by funds provided through a grant from the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project (JD), in part by the National Research Initiative Competitive Grant no. 2009-65300-05640 from the USDA National Institute of Food and Agriculture (JD), and in part by USDA-ARS CRIS project 5442-22000-030-00D (SC).

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Authors and Affiliations

  1. USDA-ARS, Biosciences Research Laboratory, 1605 Albrecht Blvd N, Fargo, ND, 58102-2765, USA

    Kristin Simons & Shiaoman Chao

  2. Department of Plant Sciences, University of California, Davis, CA, 95616, USA

    Zewdie Abate, Wenjun Zhang & Jorge Dubcovsky

  3. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA

    Matt Rouse & Yue Jin

  4. USDA-ARS, Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, 55108, USA

    Yue Jin

  5. Department of Plants Sciences, North Dakota State University, Fargo, ND, 58102, USA

    Elias Elias

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  1. Kristin Simons
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  2. Zewdie Abate
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  3. Shiaoman Chao
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  4. Wenjun Zhang
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  5. Matt Rouse
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  6. Yue Jin
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  7. Elias Elias
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  8. Jorge Dubcovsky
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Corresponding author

Correspondence to Shiaoman Chao.

Additional information

Communicated by C. Feuillet.

K. Simons and Z. Abate contributed equally to this work.

A comment to this article can be found online at http://dx.doi.org/10.1007/s00122-010-1495-2.

Electronic supplementary material

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122_2010_1444_MOESM1_ESM.pdf

Resistance sources used, and the pedigree and year of release information for cultivars and germplasm developed in North Dakota durum wheat breeding program. Boxes indicate founders for the germplasm used in the study, and circles indicate the resistant parents used in the mapping populations (PDF 191 kb)

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Simons, K., Abate, Z., Chao, S. et al. Genetic mapping of stem rust resistance gene Sr13 in tetraploid wheat (Triticum turgidum ssp. durum L.). Theor Appl Genet 122, 649–658 (2011). https://doi.org/10.1007/s00122-010-1444-0

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  • DOI: https://doi.org/10.1007/s00122-010-1444-0

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