Abstract
A barley core collection can be studied extensively and the derived information can be used to identify loci/genes for the genetic improvement of quantitative and qualitative traits. To assess genetic diversity, allelic variation and population structure of Egyptian barley, 134 barley genotypes collected from a different region along with 19 cultivated genotypes obtained from of the Egyptian Agricultural Research Center. All genotypes were analyzed with 261 polymorphic SSR and SNP alleles. The mean number of alleles per locus was 4 and PIC was 0.49, while the level of genetic diversity was 0.55 ranging from 0.03 to 0.82. The genotypes were assigned to three subpopulations that were consistent with their origins. The genetic variation within population was higher (51%) than among population at the molecular levels (FST = 0.491 when P < 0.10). The level of polymorphic variation was highest in subpopulations-II, due to collected from different regions with different ear-types thus, expected to contain more diversity than local genotypes in subpopulations-I and subpopulations-III. The structured study found that the 153 barley genotypes are in harmony with clustering approaches using the SSR and SNP genotypic data in a neighbor-joining tree and principal components analysis, which identified three subpopulations. These results demonstrated genetic diversity among the Egyptian barley genotypes can be applied to suggest approaches, such as association analysis, classical mapping population development, and parental line selection in breeding programs. Therefore, it is necessary to use the exotic genotypes as the genetic resources for developing new barley cultivars in Egypt.
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This work was supported by the Agricultural Research Center and the Ministry of Higher Education in Egypt, and the Institute of Genetic Resources in Kyushu University, Japan.
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AE developed the experimental design, processed data, bioinformatics analyses, and prepared the early stages of the manuscript. TK provided the facilities and markers. KA provided germplasm management, experimental design, and coordination. CQ, RB, and LC reviewed the manuscript for important intellectual content, and final version.
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Elakhdar, A., Kumamaru, T., Qualset, C.O. et al. Assessment of genetic diversity in Egyptian barley (Hordeum vulgare L.) genotypes using SSR and SNP markers. Genet Resour Crop Evol 65, 1937–1951 (2018). https://doi.org/10.1007/s10722-018-0666-x
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DOI: https://doi.org/10.1007/s10722-018-0666-x