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Phylogenetic implications of the internal transcribed spacers of nrDNA and chloroplast DNA fragments of Musa in deciphering the ambiguities related to the sectional classification of the genus

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Abstract

The genus Musa L. comprises of economically important bananas and has been divided into five sections based on their chromosome number and morphological characteristics viz., Australimusa, Callimusa, Eumusa, Rhodochlamys and Ingentimusa. However, this sectional classification has long been disputed. In this study, we present data on sequenced multiple DNA fragments from the maternally inherited chloroplast genome (atpB-rbcL spacer, trnK-matK intron and psbK-psbI spacer) and the biparentally inherited internal transcribed spacers of nuclear ribosomal DNA of three species of Musa along with data available in GenBank database to understand their implications on the sectional relationship and phylogeny of the genus Musa. In our findings, none of the five sections of Musa previously defined based on morphology was recovered in the molecular phylogeny analysis using cpDNA and nrDNA. Instead, the results corroborate with the suggestion that sectional classification in the genus Musa should be reviewed by combining together the section Eumusa and Rhodochlamys as one and section Australimusa and Callimusa as a single different section, along with that of Ingentimusa.

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Acknowledgments

We are thankful to the Head, Department of Biotechnology and Bioinformatics, North-Eastern Hill University for providing necessary facilities to carry out this research work. Immense appreciation are also accorded to Dr. Jeremy Dkhar, DST-INSPIRE faculty, Jawaharlal Nehru University, New Delhi and all the Plant Biotechnology Laboratory members, North-Eastern Hill University for all the assistance and motivation rendered.

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  1. Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India

    Animos Lamare & Satyawada Rama Rao

  2. Department of Biology, University of Mazandaran, Babolsar, Iran

    Arman Mahmoudi Otaghvari

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  1. Animos Lamare
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Correspondence to Satyawada Rama Rao.

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Lamare, A., Otaghvari, A.M. & Rao, S.R. Phylogenetic implications of the internal transcribed spacers of nrDNA and chloroplast DNA fragments of Musa in deciphering the ambiguities related to the sectional classification of the genus. Genet Resour Crop Evol 64, 1241–1251 (2017). https://doi.org/10.1007/s10722-016-0433-9

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