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Diversity of Citrus depressa Hayata (Shiikuwasha) revealed by DNA analysis

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Abstract

Citrus depressa Hayata is an indigenous mandarin species on the Ryukyu Islands located in the subtropical region of Japan. We deduced its phylogenetic relationships by evaluating accessions grown on various Ryukyu Islands via cleaved amplified polymorphic sequence analysis of cpDNA and sequence-related amplified polymorphism (SRAP). The cpDNA results indicated that C. depressa could be classified into two types. SRAP revealed patterns of diversity within C. depressa consistent with our cpDNA results. These results indicate that maternal origin may influence or is correlated with the constitution of the nuclear genome of C. depressa. Another Japanese mandarin species, Citrus tachibana (Makino) Tanaka was distinguished from C. depressa by SRAP markers. Moreover, both C. depressa and C. tachibana could be distinguished from other Citrus species. Our results suggest that Japanese mandarin possesses a characteristic genome with the genus Citrus.

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Author notes

  1. Sayuri Teramoto

    Present address: JICA Ethiopia Office, P. O. Box 5384, Addis Ababa, Ethiopia

Authors and Affiliations

  1. Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima, 890-0065, Japan

    Masashi Yamamoto, Akiko Takakura & Aika Tanabe

  2. Faculty of Agriculture, University of the Ryukyus, Senbaru, Nishihara-cho, Nakagami-gun, Okinawa, 903-0213, Japan

    Sayuri Teramoto

  3. Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, Okitsunakacho, Shimizu, Shizuoka, 424-0292, Japan

    Masayuki Kita

Authors
  1. Masashi Yamamoto
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  2. Akiko Takakura
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  3. Aika Tanabe
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  4. Sayuri Teramoto
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  5. Masayuki Kita
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Correspondence to Masashi Yamamoto.

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Yamamoto, M., Takakura, A., Tanabe, A. et al. Diversity of Citrus depressa Hayata (Shiikuwasha) revealed by DNA analysis. Genet Resour Crop Evol 64, 805–814 (2017). https://doi.org/10.1007/s10722-016-0403-2

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  • DOI: https://doi.org/10.1007/s10722-016-0403-2

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