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Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L.

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Abstract

Sclerotinia stem rot, caused by fungus Sclerotinia sclerotiorum, is one of the most devastating diseases in rapeseed (Brassica napus L.). We report the identification of Quantitative trait loci (QTL) involved in the resistance to S. sclerotiorum in two segregating populations of DH lines: the HUA population, derived from a cross between a partially resistant Chinese winter line (Hua dbl2) and a susceptible European spring line (P1804); and the MS population, derived from a partially resistant French winter cultivar (Major) and a susceptible Canadian spring cultivar (Stellar). A petiole inoculation technique and two scoring methods, days to wilt (DW) and stem lesion length (SLL), were used for the resistance assessment. A total of eight genomic regions affecting resistance were detected in the HUA population, with four of these regions affecting both measures of resistance. Only one region, which affected both measurements, was detected in the MS population. Individual QTL explained 6–22% of the variance. At five of the QTL from both populations, alleles from the resistant parent contributed to the resistance. QTL on N2 from the HUA population had the highest LOD score and R 2 value and was detected for SLL in the first evaluation. The N12 resistance allele in Hua dbl2 was detected in a region containing a homeologous non-reciprocal transposition (HNRT) from the resistance-containing portion of N2. This result suggests that QTL in the N12.N2 HNRT enhanced the resistance of Hua dbl2 by increasing the dosage of resistance genes. The relationship of QTL from different genetic backgrounds and their associations with other agronomic traits are discussed.

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Acknowledgements

We thank Dr. Glen Hartman, USDA/ARS, University of Illinois for providing the S. sclerotiorum isolate. This work was funded by the USDA Sclerotinia Initiative.

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Author notes

  1. Joshua A. Udall

    Present address: Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, IA, 50011, USA

  2. Thomas C. Osborn

    Present address: Seminis Vegetable Seeds, 37437 State Highway 16, Woodland, CA, 95695, USA

Authors and Affiliations

  1. Department of Agronomy, University of Wisconsin, Madison, WI, 53706, USA

    Jianwei Zhao, Joshua A. Udall, Pablo A. Quijada & Thomas C. Osborn

  2. Department of Plant Pathology, University of Wisconsin, Madison, WI, 53706, USA

    Craig R. Grau

  3. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, China

    Jianwei Zhao & Jinling Meng

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  1. Jianwei Zhao
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Correspondence to Jianwei Zhao.

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Communicated by C. Möllers

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Zhao, J., Udall, J.A., Quijada, P.A. et al. Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. . Theor Appl Genet 112, 509–516 (2006). https://doi.org/10.1007/s00122-005-0154-5

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