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EST-SSR cross-amplification and genetic similarity in Onobrychis genus

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Abstract

EST-SSR from Medicago truncatula Gaertn. and Glycine max (L.) Merr. were tested for transferability in various species of Onobrychis (O. pyrenaica Sennen, O. argentea Boiss. and O. viciifolia Scop.). Repeatable amplification was obtained for 81% of the microsatellites and 52% were polymorphic. Six selected SSRs from M. truncatula were used to fingerprint and estimate the genetic similarity of a set of 23 accessions of O. viciifolia. PCA analysis discriminated among the different Onobrychis species and the sainfoin accessions were clustered in a single major group. This grouping is discussed in terms of the history of cultivation of sainfoin in Spain. The selected SSRs will allow fingerprinting and genetic studies in Onobrychis species, solving the lack of available SSR markers in this species.

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Acknowledgments

We gratefully acknowledge T. Bespín, A. M. Cachi, S. Sancho and M. E. Guerra for technical assistance; Dr F. Fillat for expert advices in collecting wild species. This work has been partly supported by the Marie Curie Actions Project no MRTN-CT-2006-035805 and by the DGA Grupo de Excelencia A-11.

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  1. Centro de Investigación y Tecnología Agroalimentaria de Aragón, Avda. Montañana, 930, 50059, Zaragoza, Spain

    Samir Demdoum, Fernando Muñoz, Ignacio Delgado, José Valderrábano & Ana Wünsch

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  1. Samir Demdoum
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  2. Fernando Muñoz
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  3. Ignacio Delgado
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  4. José Valderrábano
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  5. Ana Wünsch
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Correspondence to Samir Demdoum.

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Demdoum, S., Muñoz, F., Delgado, I. et al. EST-SSR cross-amplification and genetic similarity in Onobrychis genus. Genet Resour Crop Evol 59, 253–260 (2012). https://doi.org/10.1007/s10722-011-9681-x

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  • DOI: https://doi.org/10.1007/s10722-011-9681-x

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