Abstract
Genetic diversity is an area of concern for sustaining crop yield. Information on genetic relatedness/diversity among Gossypium arboreum L. cultivars/genotypes is scanty. We have used random amplified polymorphic DNA (RAPD) analysis to assess the genetic divergence/relationship among 30 genotypes/cultivars of G. arboreum. Of 45 primers surveyed, 63% were polymorphic. Out of the total number of loci amplified, 36% were polymorphic. The calculated genetic similarity between the cultivars/genotypes was in the range of 47.05–98.73%. Two genotypes, HK-244 and Entry-17, were the most distantly related. The average genetic relatedness among all the genotypes was 80.46%. However, most of the cultivated varieties showed a close genetic relationship, indicating a narrow genetic base in comparison to the non-cultivated germplasm. The calculated coefficients were used to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA) algorithm, which grouped the genotypes/cultivars into two major and three smaller clusters. The study is the first comprehensive analysis of the genetic diversity of G. arboreum germplasm and identifies cultivars that will be useful in extending the genetic diversity of cultivated varieties and future genome mapping projects.
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Acknowledgement
We thank the Pakistan Agriculture Research Council (PARC), Islamabad, Pakistan for financial support under the ALP programme. We are also thankful to the Director cotton CRI Faisalabad for providing us the cottonseed of G arboreum genotypes. We are grateful to Prof. Dr. Rob W. Briddon (employed at NIBGE under the Higher Education Commission [Pakistan] ‘‘Foreign Faculty Hiring’’ scheme) for critical reading of the manuscript and Peter Long (University of Bath) for his help in compiling the map of Pakistan.
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Mehboob-ur-Rahman, Yasmin, T., Tabbasam, N. et al. Studying the extent of genetic diversity among Gossypium arboreum L. genotypes/cultivars using DNA fingerprinting. Genet Resour Crop Evol 55, 331–339 (2008). https://doi.org/10.1007/s10722-007-9238-1
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DOI: https://doi.org/10.1007/s10722-007-9238-1