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Sampling strategies and screening of chickpea (Cicer arietinum L.) germplasm for salt tolerance

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Abstract

Six hundred accessions of chickpea (Cicer arietinum L.) landraces and its wild relatives from 28 different countries, available at Australian Temperate and Field Crops Collection (ATFCC) were screened for tolerance to salt under greenhouse conditions using three sampling strategies; (1) random sampling of 200 accessions from different countries, (2) restricted random sampling of 200 accessions from geographical regions with salinity problems and high diversity (Middle East and West & South Asia) and (3) as for strategy 1 but with a reduced representation of accessions from the geographical regions used in strategy 2. Degree of salt tolerance was based on necrosis scores and shoot biomass reduction relative to unstressed controls at harvest after subjecting stressed plants to salt treatment from 21 to 42 days after sowing. There was a wide variation in salinity tolerance determined by both measures. For sampling strategies 1, 2 and 3 respectively; 24, 28 and 14% of accessions were salt tolerant. Accessions from the middle east and south Asian (regions with salinity problem, a long history of chickpea cultivation and high diversity) gave a higher probability (P < 0.01) of getting salt tolerant accessions.

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Acknowledgements

We are grateful for Melbourne Research Scholarship (MRS) and pulse products project of Department of Primary Industries (DPI) Horsham, Victoria (Australia) and a study scholarship by Bunda College–NORAD Project (Malawi). We are also grateful to Gabriela Borgognone (DPI –Horsham) for advice on experimental design and statistical analysis.

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Authors and Affiliations

  1. Joint Centre for Crop Innovation, School of Agriculture and Food Systems, University of Melbourne, Private Bag 260, Horsham, Victoria, 3401, Australia

    Moses F. A. Maliro, James F. Kollmorgen & Chris Pittock

  2. Department of Primary Industries, Private Bag 260, Horsham, Victoria, 3401, Australia

    David McNeil & Bob Redden

  3. School of Agricultural Sciences, University of Tasmania, Private Bag 54, Hobart, Tasmania, 7001, Australia

    David McNeil

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  1. Moses F. A. Maliro
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  2. David McNeil
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  4. James F. Kollmorgen
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Correspondence to Moses F. A. Maliro.

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Maliro, M.A., McNeil, D., Redden, B. et al. Sampling strategies and screening of chickpea (Cicer arietinum L.) germplasm for salt tolerance. Genet Resour Crop Evol 55, 53–63 (2008). https://doi.org/10.1007/s10722-007-9214-9

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  • DOI: https://doi.org/10.1007/s10722-007-9214-9

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