Abstract
Since Upland cotton (Gossypium hirsutum) is known to have relatively low levels of genetic diversity, a better understanding of variation and relationships among possible sources of novel genes would be valuable. Therefore, analysis of genetic variation of the genus Gossypium, especially the diploids, which are the putative donors of the A and D genomes for the commercially important allotetraploid cottons (AADD), G. hirsutum and G. barbadense, could provide important information about the feasibility of using these genetic resources for cotton improvement. The primary objective of this study was to analyze the genetic diversity in A-genome diploid cotton species, G. herbaceum (A1) and G.␣arboreum (A2) by using microsatellite markers. Forty-one A-genome germplasm accessions were evaluated with 32 microsatellite loci. Genetic similarities between A1 and A2 ranged from 0.62 to 0.86 with a mean of 0.70. Within each A-genome species similarities ranged from 0.80 to 0.97 with a mean of 0.89 for A1 and from 0.82 to 0.98 with a mean of 0.89 for A2. A UPGMA tree and principal coordinate analysis based on genetic similarity matrices showed distinct clusters consistent with the genomic groups.
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Acknowledgements
We are grateful to Dr. Ray Jackson for his assistance in the cytogenetic analysis. We also thank the Germplasm Unit at the USDA-ARS at College Station, Texas, for providing the cotton germplasm accessions. DNA sequencing was performed at the Texas Tech University Center for Biotechnology and Genomics Core Facility. This research was supported by grants from the Texas Advanced Technology Program and the Texas Cotton Research Institute.
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Kebede, H., Burow, G., Dani, R.G. et al. A-genome cotton as a source of genetic variability for Upland cotton (Gossypium hirsutum). Genet Resour Crop Evol 54, 885–895 (2007). https://doi.org/10.1007/s10722-006-9157-6
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DOI: https://doi.org/10.1007/s10722-006-9157-6