Abstract
The genus Nicotiana is a member of the nightshade (Solanaceae) family, and is comprised of 70 currently recognized, naturally occurring species. Genetic variability within N. tabacum L., the species of primary economic importance, was likely affected by several genetic bottlenecks. Nicotiana tabacum is a classic amphidiploid that arose after chance interspecific hybridization between N. sylvestris Spegazinni et Comes and a member of section Tomentosae, likely N. tomentosiformis Goodspeed, N. otophora Grisebach, or an introgressive hybrid between the two. Only a fraction of the genetic variability that existed in the diploid progenitor gene pools probably entered into N. tabacum. Genetic drift, coupled with natural and human selection, subsequently resulted in the formation of narrow genetic pools corresponding to modern commercial market classes. Genetic variability in Nicotiana has gained increased attention in recent years because of investment in Nicotiana genomics research, interest in development of tobacco products with reduced harm characteristics, and concentration on using Nicotiana species for plant-based production of commercially useful proteins. A storehouse of genetic diversity for N. tabacum is available in approximately 1,900 accessions maintained by the United States Nicotiana Germplasm Collection. Seeds of 224 accessions representing 59 wild Nicotiana species are also maintained. The collection is currently maintained by North Carolina State University and is part of the United States National Plant Germplasm System (NPGS). The collection’s curator satisfies hundreds of seed requests made annually by scientists using Nicotiana germplasm for basic biological investigations and by researchers in the area of applied tobacco science.
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References
Anastasia GE (1914) Araldica Nicotianae. Nuove ricerche intorno alla filogenesi delle varieta di N. tabacum L. R.Ist. Sper. Coltiv. Tab., Scafati
Aoki S, Ito M (2000) Molecular phylogeny of Nicotiana (Solanaceae) based on the nucleotide sequence of the matK gene. Plant Biol 2:316–324
Apple JL (1962) Transfer of resistance to black shank (Phytophthora parasitica var. nicotianae from Nicotiana plumbaginifolia to N. tabacum. Phytopath 52:1(Abstr.)
Bindler G, Martin F, Renaud L, Kaelin E, Donini P, Gandani F and Rossi L (2005) Microsatellite markers for tobacco genetic fingerprinting and variety identification. Plant and Animal Genomes XIII Conference, San Diego, CA, P136 (Abstract) (abstract available at http://www.intl-pag.org/13/abstracts/PAG13_P136.html)
Bland MM, Matzinger DF, Levings, CS, III (1985) Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species. Theor Appl Genet 69:535–541
Bowman DT (1996) History of the Regional Minimum Standards Program for the release of flue-cured tobacco varieties in the United States. Tob Sci 40:99–110
Bowman DT, May OL, Calhoun DS (1996) Genetic base of upland cotton cultivars released between 1970 and 1990. Crop Sci 36:477–581
Bowman DT, Wernsman EA, Corbin TC, Tart AG (1984) Contribution of genetics and production technology to long-term yield and quality gains in flue-cured tobacco. Tob Sci 28:30–35
Brandle JD, Bai D (1999) Biotechnology: uses and applications in tobacco improvement. In: Davis DL, Nielsen MT (eds) Tobacco production, chemistry, and technology. Blackwell Science Ltd., Oxford, pp 49–65
Brooks JE (1952) The mighty leaf. Little Brown and Co., Boston MA
Bullock JF (1943) Strains of flue-cured tobacco resistant to black shank. U.S. Dept. Agric. Circ. 682
Cameron DR (1952) Inheritance in Nicotiana tabacum. XXIV. Intraspecific differences in chromosome structure. Genetics 37:288–296
Chaplin JF, Burk LG (1979) Plant propagation. In: Durbin RD (ed) Nicotiana: procedures for experimental use. USDA Technical Bulletin No. 1586
Chaplin JF, Stavely JR, Litton CC, Pittarelli GW, West WH, Jr (1982) Catalog of the Tobacco Introductions in the U.S. Department of Agriculture’s Tobacco Germplasm Collection (Nicotiana tabacum). USDA Agric. Res. Serv. Agric. Rev. Man. No. 27
Chase MW, Knapp S, Cox AV, Clarkson JJ, Butsko Y, Joseph J, Savolainen V, Parokonny AS (2003) Molecular systematics and the origin of hybrid taxa in Nicotiana (Solanaceae). Ann Bot 92:107–127
Chen ZJ, Comai L, Pikaard CS (1998) Gene dosage and stochastic effects determine the severity and direction of uniparental ribosomal RNA gene silencing (nucleolar dominance) in Arabidopsis allopolyploids. Proc Natl Acad Sci (USA) 95:14891–14896
Clayton EE (1947) A wildfire resistant tobacco. J Hered 38:35–40
Clayton EE (1958) The genetics and breeding progress in tobacco during the last 50 years. Agron J 50:352–356
Clayton EE (1969) The study of resistance to the black root disease of tobacco. Tob Sci 13:30–37
Clayton EE, Heggestad HE, Grosso JJ, Burk LG (1967) The transfer of blue mold resistance to tobacco from Nicotiana debneyi. Part I. Breeding Progress 1937–1954. Tob Sci 11:91–99
Comes O (1899) Monographie du genre Nicotiana comprenant le classement botanique des tabacs industriels. Atti del R. Instituto d’Incoraggiamento di Napoli, ser. V. vol. 1
Danert S (1961) Zur Systematik von Nicotiana tabacum L. Kulturpflanze 9:287–363
Daniell H, Streatfield SJ, Wycoff K (2001) Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci 5:219–226
East EM, Jones DF (1921) Round tip tobacco – a plant “Made to Order”. J Heredity 12:50–56
Feulner G, Lommel SA, Opperman CH, Sosinski B, Burke M, Salstead A, Gandani F, Hayes A (2003) An expressed sequence tag (EST) project on Nicotiana benthamiana. Plant & Animal Genomes XI Conference, San Diego, CA, P31 (Abstract) (abstract available at http://www.intl-pag. org/pag/11/abstracts/P01_P31_XI.html)
Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 7:152–158
Garner WW, Allard H, Clayton EE (1936) Superior germplasm in tobacco. In: 1936 Yearbook of agriculture. USDA, Washington, DC, pp 785–830
Gerstel DU (1961) Essay on the origin of tobacco. Tob Sci 5:15–17
Gerstel DU, Sisson VA (1995) Tobacco. In: Smartt J, Simmonds NW (eds) Evolution of crop plants, 2nd edn. John Wiley & Sons, Inc., New York, pp 458–463
Gizlice Z, Carter TE Jr, Burton JW (1993) Genetic diversity in North American soybean: I. Multivariate analysis of founding stock and relation to coefficient of parentage. Crop Sci 33:614–620
Goodspeed TH (1954) The genus Nicotiana. Chronica Botanica, Waltham Massachusetts
Hayes HK, East EM, Beinhart EG (1913) Tobacco breeding in Connecticut. Conn. Agr. Exp. Sta. Bull. No. 176
Holmes FO (1938) Inheritance of resistance to tobacco-mosaic disease in tobacco. Phtyopath 28:553–561
Horn ME, Woodard SL, Howard JA (2004) Plant molecular farming: systems and products. Plant Cell Rep 22:711–720
Japan Tobacco Inc. (1994) The genus Nicotiana illustrated. Japan Tobacco Inc., Tokyo Japan
Jeffrey RN (1959) Alkaloid composition of species of Nicotiana. Tob Sci 3:89–93
Johnson CS, Reed TD (1994) Tobacco. In: Arntzen CJ, Ritter EM (eds) Encyclopedia of Agricultural Science, vol 1. Academic Press, New York, pp 323–336
Jones GL, Mann TJ (1958) Variations in Hicks variety of flue-cured tobacco. Tob Sci 2:95–98
Julio E, Verrier J-L, Dorlhac de Borne F (2006) Development of SCAR markers linked to three disease resistances based on AFLP within Nicotiana tabacum L. Theor Appl Genet 112:335–346
Kawatoko K (1998) Ecological studies on the geographical distribution of the genus Nicotiana. Bulletin of the Leaf Tobacco Research Laboratory Japan Tobacco Inc., Tochigi Japan
Kempthorne O (1957) An introduction to genetic statistics. John Wiley and Sons, New York, New York
Kenton A, Parokonny AS, Gleba YY, Bennett MD (1993) Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics. Mol Gen Genet 240:159–169
Kitamura S, Inoue M, Shikazono N, Tanaka A (2001) Relationships among Nicotiana species revealed by the 5S rDNA spacer sequence and fluorescence in situ hybridization. Theor Appl Genet 103:678–686
Knapp S, Chase MW, Clarkson JJ (2004) Nomenclatural changes and a new section classification in Nicotiana (Solanaceae). Taxon 52:73–82
Knauft DA, Gorbet DW (1989) Genetic diversity among peanut cultivars. Crop Sci 29:1417–1422
Lester RN, Hawkes JG (2001) Solanaceae. In: Hanelt P and Institute of Plant Genetics and Crop Plant Research (eds) Mansfeld’s encyclopedia of agricultural and horticultural crops (except ornamentals), vol 4. Springer, Berlin, Heidelberg, pp 1790–1856
Lewis RS (2005) Transfer of resistance to potato virus Y (PVY) from Nicotiana africana to Nicotiana tabacum: possible influence of tissue culture on the rate of introgression. Theor Appl Genet 110:678–687
Lim KY, Matyasek R, Lichtenstein CP, Leitch AR (2000) Molecular and cytogenetic analyses and phylogenetic studies in the Nicotiana section Tomentosae. Chromosoma 109:245–258
Litton CC, Stokes GW (1964) Outcrossing in burley tobacco. Tob Sci 8:113–115
Liu B, Vega JM, Segal G, Abbo S, Rodova M, Feldman M (1998) Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. I. Changes in low-copy noncoding DNA sequences. Genome 41:272–277
Mallah GS (1943) Inheritance in Nicotiana tabacum. XVI. Structural differences among the chromosomes of a selected group of varieties. Genetics 28:525–532
Mann TJ, Weybrew JA (1958) Inheritance of alkaloids in hybrids between flue-cured tobacco and related amphidiploids. Tob Sci 2:29–34
Mann TJ, Weybrew JA, Matzinger DF, Hall JL (1964) Inheritance of the conversion of nicotine to nornicotine in varieties of Nicotiana tabacum L. and related amphidiploids. Crop Sci 4:349–353
Martin JM, Blake TK, Hockett EA (1991) Diversity among North American spring barley cultivars based on coefficients of parentage. Crop Sci 31:1131–1137
McMurtrey JE Jr, Wilson DB, Pointer JP (1960) Natural crossing of tobacco under Maryland conditions. Tob Sci 4:243–247
Merxmüller H, Buttler KP (1975) Nicotiana in der afrikanischen Namib – ein pflanzengeographisches and phylogenetisches Rätsel. Mitt Bot München 12:91–104
Milla SR, Isleib TG, Stalker HT (2005) Taxonomic relationships among Arachis sect. Arachis species as revealed by AFLP markers. Genome 48:1–11
Murad L, Lim KY, Christopodulou V, Matyasek R, Lichtenstein CP, Kovarik A, Leitch AR (2002) The origin of tobacco’s T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanceae). Am J Bot 89:921–928
Murphy JP, Cox TS, Rodgers DM (1986) Cluster analysis of red winter wheat cultivars based upon coefficients of parentage. Crop Sci 26:672–676
Murphy JP, Cox TS, Rufty RC, Rodgers DM (1987) A representation of the pedigree relationships among flue-cured tobacco cultivars. Tob Sci 31:70–75
Okamuro JK, Goldberg RB (1985) Tobacco single-copy DNA is highly homologous to sequences present in the genomes of its diploid progenitors. Mol Gen Genet 198:290–298
Olmstead RG, Palmer JD (1991) Chloroplast DNA and systematics of the Solanaceae. In: Hawkes JG, Lester RN, Nee M, Estrada N (eds) Solanaceae III: taxonomy, chemistry, evolution. Royal Botanic Gardens, Kew, London, pp 161–168
Opperman CH, Lommel SA, Burke M, Carlson J, George C, Gove S, Houfek TD, Jefferys S, Kalat S, King R, Levin J, Little PC, Lumpkin A, Ross T, Salstead A, Scholl E, Sosinski B, Stevens PJ, Zekanis S, Frelinger J, Lakey N, Bidell J, Budiman A, Hayes A (2005) The Tobacco Genome Initiative: A Gene Discovery Platform. Plant & Animal Genomes XIII Conference, San Diego, CA, P027 (Abstract) (abstract available at http://www.intl-pag.org/pag/13/abstracts/PAG13_P027.html)
Opperman CH, Lommel SA, Burke M, Carlson J, George C, Gove S, Graham S, Houfek TD, Kalat S, Little PC, Lumpkin A, Redman L, Ross T, Schaffer R, Scholl E, Stephens PJ, Windham E, Zekanis SH, Lakey N, Bidell J, Budiman A (2006) Update on the Tobacco Genome Initiative: A Gene Discovery Platform. Plant & Animal Genomes XIV Conference, San Diego, CA, P30 (Abstract) (abstract available at http://www.intl-pag.org/pag/14/abstracts/PAG14_P30.html)
Opperman CH, Lommel SA, Sosinski B, Burke M, Lakey N, He L, Brierley R, Salstead A, Gadani F, Hayes A (2003) The Tobacco Genome Initiative. Plant & Animal Genomes XI Conference, San Diego, CA, P32 (Abstract) (abstract available at http://www.intl-pag.org/pag/11/abstracts/P01_P32_XI.html)
Ren N, Timko MP (2001) AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana species. Genome 44:559–571
Riechers DE, Timko MP (1999) Structure and expression of the gene family encoding putrescine N-methyltransferase in Nicotiana tabacum: new clues to the evolutionary origin of cultivated tobacco. Plant Mol Biol 41:387–401
Roath WW (1989) Evaluation and enhancement. Plant Breed Rev 7:183–211
Rossi L, Bindler G, Pijnenburg H, Isaac PG, Giraud-Henry I, Mahe M, Orvain C, Gadani F (2001) Potential of molecular marker analysis for variety identification in processed tobacco. Plants, Varieties, and Seeds 14:89–101
Schranz ME, Osborne TC (2004) De novo variation in life-history traits and responses to growth conditions of resynthesized polyploid Brassica napus (Brassicaceae). Am J Bot 91:174–183
Setchell WA (1921) Aboriginal tobaccos. Am Anthropol 23:397–414
Shamel AD, Cobey WW (1907) Tobacco breeding. USDA Bureau Plant Ind. Bull. No. 96
Shands HL, Fitzgerald PJ, Eberhart SA (1989) Program for plant germplasm preservation in the United States: the U.S. National Plant Germplasm System. In: Knutson P, Stoner AK (eds) Biotic diversity and germplasm preservation: global imperatives. Kluwer Academic Publishers, Dordrecht The Netherlands, pp 97–115
Shands HL (1995) The U.S. National Plant Germplasm System. Can J Plant Sci 75:9–15
Sisson VA, Saunders JA (1982) Alkaloid composition of the USDA tobacco (Nicotiana tabacum L.) introduction collection. Tob Sci 26:117–120
Smith TE, Clayton EE, Moss EG (1945) Flue-cured tobacco resistant to bacterial (Granville) wilt. USDA Circ. No. 727
Smith HH, Smith CR (1942) Alkaloids in certain species and interspecific hybrids of Nicotiana. J Agric Res 65:347–359
Sneller CH (1994) Pedigree analysis of elite soybean lines. Crop Sci 34:1515–1522
Song KM, Lu P, Tang KL, Osborne TC (1995) Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proc Nat Acad Sci (USA) 92:7719–7723
Spinden HJ (1950) Tobacco is American. The New York Public Library, New York
Steinberg RA (1959) Factors influencing reproduction of some Nicotiana species in the greenhouse and field. Tob Sci 3:131–135
Tilley NM (1948) The bright-tobacco industry 1860–1929. The Univ. of North Carolina Press, Chapel Hill
Tso TC (1990) Production, physiology, and biochemistry of tobacco plant. Ideals, Inc., Beltsville Maryland
Uchiyama H, Chen K, Wildman SG (1977) Polypeptide composition of fraction I protein as an aid in the study of plant evolution. Stadler Genet Symp 9:83–99
United States Department of the Interior (1883) Report on the productions of agriculture as returned at the tenth census (June 1, 1880). United States Government Printing Office, Washington, DC
Valleau WD, Stokes GW, Johnson EM (1960) Nine years’ experience with the Nicotiana longiflora factor for resistance to Phytophthora parasitica var. nicotianae in the control of black shank. Tob Sci 4:92–94
Wernsman EA, Rufty RC (1987) Tobacco. In: Fehr WR (ed) Principles of cultivar development, vol 2, Crop Species. Macmillian Publishing Company, New York, pp 669–698
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Lewis, R.S., Nicholson, J.S. Aspects of the evolution of Nicotiana tabacum L. and the status of the United States Nicotiana Germplasm Collection. Genet Resour Crop Evol 54, 727–740 (2007). https://doi.org/10.1007/s10722-006-0024-2
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DOI: https://doi.org/10.1007/s10722-006-0024-2