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Determining genetic diversity between lines of Vigna unguiculata subspecies by AFLP and SSR markers

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Abstract

AFLP (Amplified Fragment Length Polymorphisms) and SSR (Simple Sequence Repeat) markers were utilized to assess genetic diversity and relatedness between Vigna unguiculata subspecies. Three AFLP primer combinations and 10 SSR primer sets successfully identified closely related accessions, and the presence of heterogeneity in some accessions. AFLP methodology was successful in separating different species of Vigna. However, the level of intra-subspecies variation was as great as was the interspecies variation with both marker methods. The number of markers employed was insufficient to successfully group the subspecies into distinct clades.

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References

  • J. Felsenstein (1993) PHYLIP (Phylogeny Inference Package) University of Washington, Department of Genetics Seattle

    Google Scholar 

  • R.L. Fery (2002) New opportunities in Vigna J. Janick A. Whipkey (Eds) Trends in New Crops and New Uses ASHS Press AlexandriaVA. 424–428

    Google Scholar 

  • C.D. Li C.A. Fatokun B. Ubi B.B. Singh G.J. Scoles (2001) ArticleTitleDetermining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers Crop Sci. 41 189–197

    Google Scholar 

  • R. Marèchal J.M. Mascherpa F. Stainier (1978) ArticleTitleEtude taxonomique d’un groupe complexe d’espèces des genres Phaseolus et Vigna (Papilionaceae) sur la base de données morphologiques et polliniques, traitées par l’analyse informatique Boissiera 28 1–273

    Google Scholar 

  • M. Nei (1972) ArticleTitleGenetic distance between populations Am. Nat. 106 283–292 Occurrence Handle10.1086/282771

    Article  Google Scholar 

  • M. Nei (1987) Molecular Evolutionary Genetics Columbia University Press New York

    Google Scholar 

  • M. Nei W. Li (1979) ArticleTitleMathematical model for studying genetic variance in terms of restriction endonucleases Proc. Natl. Acad. Sci. USA 76 5269–5273 Occurrence Handle291943

    PubMed  Google Scholar 

  • R.D.M. Page (1996) ArticleTitleTREEVIEW: an application to display phylogenetic trees on personal computers Comput. Appl. Biosci. 12 357–358 Occurrence Handle8902363

    PubMed  Google Scholar 

  • R.S. Pasquet (1999) ArticleTitleGenetic relationships among subspecies of Vigna unguiculata (L.) Walp. based on allozyme variation Theor. Appl. Gene. 98 1104–1119 Occurrence Handle10.1007/s001220051174

    Article  Google Scholar 

  • Verdcourt B. 1970. Studies the Leguminosae-Papilionoideae for ‘Flora of Tropical East Africa’: IV. Kew Bull. 507–569.

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Authors and Affiliations

  1. U.S. Department of Agriculture, Agricultural Research Service, Plant Genetic Resources Conservation Unit, 1109 Experiment St., Griffin, GA, 30223, USA

    A. G. Gillaspie Jr.

  2. ARS, Plant Genetic Resources Conservation Unit, formerly USDA, 1109 Experiment St., Griffin, GA, 30223, USA

    M. S. Hopkins

  3. Plant Genetic Resources Conservation Unit, University of Georgia, 1109 Experiment St., 30223, Griffin, GA, USA

    R. E. Dean

Authors
  1. A. G. Gillaspie Jr.
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  2. M. S. Hopkins
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  3. R. E. Dean
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Correspondence to A. G. Gillaspie Jr..

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Gillaspie, A.G., Hopkins, M.S. & Dean, R.E. Determining genetic diversity between lines of Vigna unguiculata subspecies by AFLP and SSR markers. Genet Resour Crop Evol 52, 245–247 (2005). https://doi.org/10.1007/s10722-004-6693-9

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  • DOI: https://doi.org/10.1007/s10722-004-6693-9

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