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Microsatellites and RAPD Markers to Study Genetic Relationships Among Cowpea Breeding Lines and Local Varieties in Senegal

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An Erratum to this article was published on 23 September 2006

Abstract

Genetic diversity in local cowpea varieties and breeding lines from Senegal were studied using random amplified polymorphic DNA (RAPD) and microsatellite (SSR) techniques. Among the 61 RAPD primers used, twelve show polymorphism. Fifteen of the 30 microsatellite primer pairs were polymorphic, detecting one to nine alleles per locus. The RAPD and SSR data were analyzed both separately and in combination to assess relationships among genetic lines. Although RAPD provided information on levels of genetic diversity, microsatellite markers are most effective in determining the relationship among cowpea accessions and varieties. The SSR results support the genetic diversification of cowpea in Senegal and underscore their potential in elucidating patterns of germplasm diversity of cowpea in Senegal.

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Abbreviations

IITA:

International Institute of Tropical Agriculture

References

  • S. Coulibaly R.S. Pasquet R. Papa P. Gepts (2002) ArticleTitleAFLP analysis of the phenetic organization and genetic diversity of Vigna unguiculata L. Walp. reveals extensive gene flow between wild and domesticated types Theor. Appl. Genet. 104 358–366 Occurrence Handle10.1007/s001220100740 Occurrence Handle12582708 Occurrence Handle1:CAS:528:DC%2BD38XislOgtrg%3D

    Article  PubMed  CAS  Google Scholar 

  • C. Dib S. Faure C. Fizames D. Samson N. Drouot A. Vignal P. Millasseau S. Marc J. Hazan E. Seboun G. Lathrop G. Gyapay J. Morissette J. Weissenbach (1996) ArticleTitleA comprehensive genetic map of the human genome based on 5264 microsatellites Nature 380 152–154 Occurrence Handle10.1038/380152a0 Occurrence Handle8600387 Occurrence Handle1:CAS:528:DyaK28Xhs1Gns7c%3D

    Article  PubMed  CAS  Google Scholar 

  • L.R. Dice (1945) ArticleTitleMeasures of the amount of ecological association between species Ecology 26 295–302

    Google Scholar 

  • M.L. Doldi J. Vollmann T. Lelley (1997) ArticleTitleGenetic diversity in soybean as determined by RAPD and microsatellite analysis Plant Breed. 116 331–335

    Google Scholar 

  • J.J. Doyle M. Morgante S.V. Tingey W. Powell (1998) ArticleTitleSize homoplasy in chloroplast of wild perennial relatives of soybean (Glycine subgenus Glycine) Mol. Biol. Evol. 15 215–218 Occurrence Handle9491617 Occurrence Handle1:CAS:528:DyaK1cXnsFymsQ%3D%3D

    PubMed  CAS  Google Scholar 

  • J.D. Ehlers A.E. Hall (1997) ArticleTitleCowpea (Vigna unguiculata L. Walp.) Field Crops Res. 53 187–204 Occurrence Handle10.1016/S0378-4290(97)00031-2

    Article  Google Scholar 

  • H.O.A. Eloward A.E. Hall (1987) ArticleTitleInfluence of early and late nitrogen fertilization on yield and nitrogen fixation of cowpea under well-watered and dry field conditions Field Crops Res. 15 229–244

    Google Scholar 

  • L. Fall D. Diouf M.A. Fall-Ndiaye F.A. Badiane M. Gueye (2003) ArticleTitleGenetic diversity in cowpea [Vigna unguiculata (L.) Walp.] varieties determined by ARA and RAPD techniques Afr. J. Biotech. 2 48–50 Occurrence Handle1:CAS:528:DC%2BD3sXhsFCksrs%3D

    CAS  Google Scholar 

  • C.A. Fatokun D. Danesh N.D. Young (1993) ArticleTitleMolecular taxonomic relationships in the genus Vigna based on RFLP analysis Theor. Appl. Genet. 86 97–104 Occurrence Handle10.1007/BF00223813 Occurrence Handle1:CAS:528:DyaK3sXkvFWntr4%3D

    Article  CAS  Google Scholar 

  • C.A. Fatokun H.D. Mignouna M.R. Knox T.H.N. Ellis (1997) AFLP variation among cowpea varieties Agronomy. Abstracts ASA Madison, WI 156

    Google Scholar 

  • R.L. Fery (1990) ArticleTitleThe cowpea: production, utilization, and research in the United States Hort. Rev. 12 197–222

    Google Scholar 

  • T.M. Fulton J. Chungwognse S.D. Tanksley (1995) ArticleTitleMicroprep protocol for extraction of DNA from tomato and herbaceous plants Plant Mol. Biol. 13 207–209 Occurrence Handle1:CAS:528:DyaK2MXotlyhs7c%3D

    CAS  Google Scholar 

  • C.Y. He Z.Y. Zhang Y.J. Wang X.W. Zhengl D.Y. Yu S.Y. Chen J.Y. Gail (2001) ArticleTitleMicrosatellite marker analysis of a soybean recombinant inbred line NJRIKY Yi Chuan Xue Bao 28 171–81 Occurrence Handle11233262 Occurrence Handle1:CAS:528:DC%2BD3MXhsVejurk%3D

    PubMed  CAS  Google Scholar 

  • Z. Hong G.H. Miao D.P. Verma (1993) ArticleTitleDinucleotide kinase homolog from moth bean (Vigna aconitifolia) Plant Physiol. 101 1399–1400 Occurrence Handle10.1104/pp.101.4.1399 Occurrence Handle8310070 Occurrence Handle1:CAS:528:DyaK3sXks1Kntbk%3D

    Article  PubMed  CAS  Google Scholar 

  • M.B. Kwapata A.E. Hall (1985) ArticleTitleEffects of moisture regime and phosphorus on mycorrhizal infection, nutrient uptakeand growth of cowpeas Vigna unguiculata (L.) Walp Field Crops Res. 12 241–250 Occurrence Handle10.1016/0378-4290(85)90072-3

    Article  Google Scholar 

  • C.-D. Li C.A. Fatokum B. Ubi B.B. Singh G. Scoles (2001) ArticleTitleDetermining genetic similarities among cowpea breeding lines and cultivars by microsatellite markers Crop Sci. 41 189–197 Occurrence Handle1:CAS:528:DC%2BD3MXitlKmtb4%3D

    CAS  Google Scholar 

  • A. Mengoni A. Gori M. Bazzicalupo (2000) ArticleTitleUse of RAPD and microsatellite (SSR) variation to assess genetic relationships among population of tetraploid alfalfaMedicago sativa Plant Breed. 118 1–7

    Google Scholar 

  • H.D. Mignouna H.D. Ng J. Ikca G. Thottapilly (1998) ArticleTitleGenetic diversity in cowpea as revealed by random amplified polymorphic DNA J. Genet. Breed. 52 151–159 Occurrence Handle1:CAS:528:DyaK1MXjsVSqsQ%3D%3D

    CAS  Google Scholar 

  • M. Nei W.H. Li (1979) ArticleTitleMathematical model for studying genetic variation in terms of restriction endonucleases Proc. Natl. Acad. Sci. USA 76 5269–5273 Occurrence Handle291943 Occurrence Handle1:CAS:528:DyaL3cXitVWn

    PubMed  CAS  Google Scholar 

  • L. Panella P. Gepts (1992) ArticleTitleGenetic relationships within Vigna unguiculata (L.) Walp. Based on isonzyme analyses Genet Res. Crop. Evol. 39 71–88

    Google Scholar 

  • R.S. Pasquet (1993) ArticleTitleVariation at isoenzyme loci in wild Vigna unguiculata (L.) Walp. (FabaceaePhaseoleae) Plant. Syst. Evol. 186 157–173 Occurrence Handle10.1007/BF00940795 Occurrence Handle1:CAS:528:DyaK3sXmt1WrtLc%3D

    Article  CAS  Google Scholar 

  • R.S. Pasquet (2000) ArticleTitleAllozyme diversity of cultivated cowpea Vigna unguiculata (L.) Walp Theor. Appl. Genet. 101 211–219 Occurrence Handle10.1007/s001220051471 Occurrence Handle1:CAS:528:DC%2BD3cXlvVyksbw%3D

    Article  CAS  Google Scholar 

  • R. Peakall S. Gilmore W. Keys M. Morgane A. Rafalski (1998) ArticleTitleCross-species amplification of soybean (Glycine max) simple sequence repeats (SSR) within the genus and other legume genera: implication for the transferability of SSRs in plant Mol. Biol. Evol. 15 1275–1287 Occurrence Handle9787434 Occurrence Handle1:CAS:528:DyaK1cXms1eks78%3D

    PubMed  CAS  Google Scholar 

  • A.H. Price K.A. Steele B.J. Moore P.B. Barraclough L.J. Clark (2000) ArticleTitleA combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability Theor. Appl. Genet. 100 49–56 Occurrence Handle10.1007/s001220050007 Occurrence Handle1:CAS:528:DC%2BD3cXht1aqtrw%3D

    Article  CAS  Google Scholar 

  • F.J. Rohlf (2000) NTSYS-pc. Numerical taxonomy and multivariate analysis system. Version 2.1 Exeter Publ. Setauket, NY

    Google Scholar 

  • J. Rongwen M.S. Akkaya A.A. Bhagwat U. Lavi P. Cregan (1995) ArticleTitleThe use of microsatellite DNA markers for soybean genotype identification Theor. Appl. Genet. 90 43–48 Occurrence Handle10.1007/BF00220994 Occurrence Handle1:CAS:528:DyaK2MXlt1eqsLk%3D

    Article  CAS  Google Scholar 

  • D. Sène (1966) ArticleTitleInventaire des principales varieties de niébé [Vigna unguiculata (L.) Walp.] cultivées au Sénégal L’Agronomie Tropicale 8 927–933

    Google Scholar 

  • R.R. Sokal C.D. Michener (1958) ArticleTitleA statistical method for evaluating systematic relationships Univ. Kansas Sci. Bull. 38 1409–1438

    Google Scholar 

  • M.M. Spencer M.A. Ndiaye M. Gueye D. Diouf M. Ndiaye P.M. Gresshoff (2000) ArticleTitleDNA-based relatedness of cowpea (Vigna unguiculata (L.) Walp.) genotypes using DNA Amplification Fingerprinting Physiol. Mol. Biol. Plants 6 81–88

    Google Scholar 

  • S.D. Tanksley N.D. Young A.H. Paterson M.W. Bonierbale (1989) ArticleTitleRFLP mapping in plant breeding: new tools for an old science Bio/Technology 7 257–264 Occurrence Handle10.1038/nbt0389-257 Occurrence Handle1:CAS:528:DyaL1MXltV2kt7k%3D

    Article  CAS  Google Scholar 

  • D. Tautz M. Renz (1984) ArticleTitleSimple sequences are ubiquitous repetitive components of eucaryotic genomes Nucleic Acids Res. 12 4127–4138 Occurrence Handle6328411 Occurrence Handle1:CAS:528:DyaL2cXks1KqurY%3D

    PubMed  CAS  Google Scholar 

  • N. Tosti V. Negri (2002) ArticleTitleEfficiency of three PCR-based markers in assessing genetic variation among cowpea (Vigna unguiculata subsp. unguiculata) landraces Genome 45 268–275 Occurrence Handle10.1139/g01-146 Occurrence Handle11962624 Occurrence Handle1:CAS:528:DC%2BD38XjtlSns7c%3D

    Article  PubMed  CAS  Google Scholar 

  • R.F. Vaillancourt N.F. Weeden J. Barnard (1993) ArticleTitleIsoenzyme diversity in the cowpea species complex Crop Sci. 33 606–613 Occurrence Handle1:CAS:528:DyaK2cXisVSgt7s%3D Occurrence Handle10.2135/cropsci1993.0011183X003300030037x

    Article  CAS  Google Scholar 

  • M.T. Webster N.G.C. Smith H. Ellegren (2002) ArticleTitleMicrosatellite evolution inferred from human-chimpanzee genomic sequence alignments P.N.A.S. 99 8748–8753 Occurrence Handle12070344 Occurrence Handle1:CAS:528:DC%2BD38XltF2htr4%3D

    PubMed  CAS  Google Scholar 

  • P. Winter T. Pfaff S.M. Udupa B. Hüttel P.C. Sharma S. Sahi R. Arreguin-Espinoza F. Weigand F.J. Muehlbauer G. Kahl (1999) ArticleTitleCharaterization and mapping of sequence-targged microsatellite sites in the chickpea (Cicer arietinum L.) genome Mol. Gen. Genet. 262 90–101 Occurrence Handle10503540 Occurrence Handle1:CAS:528:DyaK1MXmt1yitLo%3D

    PubMed  CAS  Google Scholar 

  • X. Yang C. Quiros (1993) ArticleTitleIdentification and classification of celery cultivars with RAPD markers Theor. Appl. Genet. 86 205–212 Occurrence Handle10.1007/BF00222080

    Article  Google Scholar 

  • K. Yu S.J. Park V. Poysa P. Gepts (2000) ArticleTitleIntegration of Simple Sequence Repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.) Heredity 91 429–434 Occurrence Handle1:CAS:528:DC%2BD3MXht1SrtL8%3D

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biotechnologies Végétales, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, B.P. 5005, Dakar Senegal

    Diaga Diouf

  2. Department of Biology, Virginia Polytechnic Institute and State University Blacksburg, 24061-0406, Virginia, USA

    Khidir W. Hilu

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  1. Diaga Diouf
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  2. Khidir W. Hilu
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Correspondence to Khidir W. Hilu.

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An erratum to this article is available at http://dx.doi.org/10.1007/s10722-006-0021-5.

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Diouf, D., Hilu, K.W. Microsatellites and RAPD Markers to Study Genetic Relationships Among Cowpea Breeding Lines and Local Varieties in Senegal. Genet Resour Crop Evol 52, 1057–1067 (2005). https://doi.org/10.1007/s10722-004-6107-z

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  • DOI: https://doi.org/10.1007/s10722-004-6107-z

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