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Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry

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Abstract

Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presence within CCA, and their possible structural characteristics, were previously unknown. In this study, GAG fractions containing HS/heparin were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). This revealed that, in addition to the eight commonly seen HS disaccharides, the four rare N-unsubstituted disaccharides were also detected in significant quantities. The disaccharide compositions varied significantly between HS/heparin fractions indicating chains with differing domain structures. This novel structural information may lead to a better understanding of the biological activities (i.e. anticoagulation and antitumor action) of CCA.

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Abbreviations

CCA:

Colla corii asini

EIC:

extracted ion chromatogram

GAG:

glycosaminoglycan

GlcNH3 + :

N-unsubstituted glucosamine

GlcNAc:

N-acetyl glucosamine

GlcNS:

N-sulfated glucosamine

HS:

heparan sulfate

LC-MS-ITTOF:

liquid chromatography-ion trap/time-of-flight mass spectrometry

NA domain:

N-acetylated domain

NA/NS domain:

transition region

NaCl:

sodium chloride

UA:

uronic acid

ΔUA:

4,5-unsaturated uronate (generated by heparinase excision)

S-domain:

highly sulfated domain

SE-HPLC:

size-exclusion HPLC

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Authors and Affiliations

  1. Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou, 350002, People’s Republic of China

    Jiayan Du, Su Liu, Jianghui Lin, Lilong Jiang & Zheng Wei

  2. College of Biological Science and Engineering, Fu Zhou University, Fu Zhou, 350002, People’s Republic of China

    Quntao Liang & Fener Chen

Authors
  1. Jiayan Du
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  2. Su Liu
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  3. Quntao Liang
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  4. Jianghui Lin
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  5. Lilong Jiang
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  6. Fener Chen
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  7. Zheng Wei
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Correspondence to Quntao Liang or Zheng Wei.

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Du, J., Liu, S., Liang, Q. et al. Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry. Glycoconj J 36, 211–218 (2019). https://doi.org/10.1007/s10719-019-09868-0

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  • DOI: https://doi.org/10.1007/s10719-019-09868-0

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