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Anti-oligosaccharide antibodies as tools for studying sulfated sialoglycoconjugate ligands for siglecs and selectins

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Abstract

Several sulfated sialoglycoconjugates have recently been shown to serve as ligands for selectins and siglecs. For instance, α2→3 sialylated 6-sulfo-Lewis x was found to serve as a ligand for selectins on skin-homing helper memory T cells, and α2→6 sialylated 6-sulfo-LacNAc to be a preferred ligand for CD22/siglec-2 on human naïve B cells. Monoclonal antibodies specific to sulfated sialoglycoconjugates are effective tools to dissect these ligands on minor subsets of human leukocytes.

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Abbreviations

CD:

cluster of differentiation

CLA:

cutaneous lymphocyte antigen

ELISA:

enzyme-linked immunosorbent assay

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Acknowledgements

This work was supported in part by grants-in-aid from the Ministry of Education, Science, Sports and Culture, Japan (19590298 and on priority areas 17015051), grants-in-aid for the Third Term Comprehensive Ten-year Strategy for Cancer Control from the Ministry of Health and Welfare, Japan, a grant for the Promotion of Fundamental Studies in Health Sciences from the National Institute of Biomedical Innovation, a grant from the Nagono Medical Foundation, and a grant from the CREST program of the Japan Science and Technology Agency.

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Authors and Affiliations

  1. Department of Molecular Pathology, Research Institute, Aichi Cancer Center, 1-1 Kanokoden, Chikusaku, Nagoya, 464-8681, Japan

    Reiji Kannagi & Naoko Kimura

  2. Department of Clinical Pathology, School of Medicine, Kyoto University, Kyoto, 606-8501, Japan

    Katsuyuki Ohmori

Authors
  1. Reiji Kannagi
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  2. Katsuyuki Ohmori
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  3. Naoko Kimura
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Correspondence to Reiji Kannagi.

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Kannagi, R., Ohmori, K. & Kimura, N. Anti-oligosaccharide antibodies as tools for studying sulfated sialoglycoconjugate ligands for siglecs and selectins. Glycoconj J 26, 923–928 (2009). https://doi.org/10.1007/s10719-008-9122-z

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  • DOI: https://doi.org/10.1007/s10719-008-9122-z

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