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Convenient and rapid analysis of linkage isomers of fucose-containing oligosaccharides by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry

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Abstract

Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) was used to analyze three pyridylamino (PA)-fucosyloligosaccharides isolated from human milk: lacto-N-fucopentaose (LNFP) I [Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc-PA], LNFP II [Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4Glc-PA], and LNFP III [Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glc-PA]. These oligosaccharides are linkage isomers. MALDI-QIT-TOF MS provides MSn spectra, which we used to characterize these PA-oligosaccharides. MS/MS/MS analysis of the non-reducing end tri-saccharide ions generated by MS/MS was able to distinguish these oligosaccharide isomers. The MALDI-QIT-TOF MS is a very convenient and rapid method, therefore, it would be useful for high throughput structural analyses of various types of pyridylaminated oligosaccharide isomers.

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Authors and Affiliations

  1. Sphingolipid Expression Laboratory, Supra Biomolecular System Research Group, RIKEN Frontier Research System, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan

    Yusuke Suzuki, Minoru Suzuki, Emi Ito, Hiroshi Ishii & Akemi Suzuki

  2. Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto, 604-8511, Japan

    Kouzou Miseki

Authors
  1. Yusuke Suzuki
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  2. Minoru Suzuki
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  3. Emi Ito
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  4. Hiroshi Ishii
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  5. Kouzou Miseki
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  6. Akemi Suzuki
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Correspondence to Yusuke Suzuki.

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Suzuki, Y., Suzuki, M., Ito, E. et al. Convenient and rapid analysis of linkage isomers of fucose-containing oligosaccharides by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. Glycoconj J 22, 427–431 (2005). https://doi.org/10.1007/s10719-005-4173-x

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  • DOI: https://doi.org/10.1007/s10719-005-4173-x

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