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Origin of the X1X1X2X2/X1X2Y sex chromosome system of Harttia punctata (Siluriformes, Loricariidae) inferred from chromosome painting and FISH with ribosomal DNA markers

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Abstract

Harttia is a genus in the subfamily Loricariinae that accommodates fishes popularly known as armored catfishes. They show extensive karyotypic diversity regarding interspecific numerical/structural variation of the karyotypes, with the presence of the XX/XY1Y2 multiple sex chromosome system, as found in H. carvalhoi. In this context, this study aimed to characterize Harttia punctata chromosomally, for the first time, and to infer the rearrangements that originated the X1X1X2X2/X1X2Y multiple sex chromosome system present in this species. The data obtained in this study, with classical (Giemsa, C-banding and AgNORs) and molecular methodologies (fluorescence in situ hybridization) and chromosome microdissection, indicated that a translocation between distinct acrocentric chromosomes bearing rRNA genes, accompanied by deletions in both chromosomes, might have originated the neo-Y chromosome in this species. The data also suggest that the multiple sex chromosome systems present in H. carvalhoi and H. punctata had an independent origin, evidencing the recurrence of chromosome alterations in species from this genus.

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Acknowledgments

The authors are grateful to Osvaldo T. Oyakawa (MZUSP) for the identification of the specimens; to Luis H. da Silva and Pedro L. Gallo for help with the sampling; to the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA—License Number: 10538-1) and the Instituto Estadual de Florestas (IEF/MG) for authorization to collect the material. This study was financed by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Fundação Araucária (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná).

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Authors and Affiliations

  1. Universidade Tecnológica Federal do Paraná, Prolongamento da Rua Cerejeira s/n. Bairro São Luís, Santa Helena, Paraná, CEP: 85892-000, Brazil

    Daniel Rodrigues Blanco

  2. Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil

    Marcelo Ricardo Vicari, Roberto Ferreira Artoni & Mara Cristina de Almeida

  3. Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Rua Universitária, 2069, Cascavel, PR, 85819-110, Brazil

    Roberto Laridondo Lui & Vladimir Pavan Margarido

  4. Departamento de Genética e Evolução, Universidade Federal de São Carlos, Rodovia Washington Luís Km 235, São Carlos, SP, 13565-905, Brazil

    Josiane Baccarin Traldi & Orlando Moreira-Filho

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  1. Daniel Rodrigues Blanco
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Correspondence to Daniel Rodrigues Blanco.

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Blanco, D.R., Vicari, M.R., Lui, R.L. et al. Origin of the X1X1X2X2/X1X2Y sex chromosome system of Harttia punctata (Siluriformes, Loricariidae) inferred from chromosome painting and FISH with ribosomal DNA markers. Genetica 142, 119–126 (2014). https://doi.org/10.1007/s10709-014-9759-4

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