Abstract
This paper describes a new technique for preparing mitotic fish chromosomes using short-term in vitro treatment with colchicine. The results show that a large number of good quality metaphases (many suitable for chromosome banding) can be obtained by this technique, which requires an average of 1 h and 30 min for all steps. The procedure considerably reduces the time normally required for chromosome preparations in fish.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gold, J.R., Li Y.C., Shipley, N.S., and Powers, P.K., J. Fish Biol.37 (1990) 563.
Alvares, M.C., Otis, J., Amores, A., and Guise, K., J. Fish Biol.39 (1991) 817.
Rivlin, K., Rachlin, J.W., and Dale, G., J. Fish Biol.26 (1985) 267.
Lozano, R., Ruiz-Rejon, C., and Ruiz-Rejon, M., Stain Technol.63 (1988) 335.
Cucchi, C., and Barufaldi, A., J. Fish Biol.37 (1990) 71.
Hsu, T.C., Human and Mammalian Cytogenetics. An Historical Perspective. Springer-Verlag, New York 1979.
Ojima, Y., and Hitotsumachi, S., Jap. J. Genet.42 (1967) 163.
Klinkhardt, M.B., Cytobios67 (1991) 193.
Howell, W.M., and Black, D.A., Experientia36 (1980) 1014.
Sumner, A.T., Exp. Cell Res.75 (1972) 304.
Kligerman, A.D., and Bloom, S.E., J. Fish. Res. Board Canada34 (1977) 266.
Author information
Authors and Affiliations
Additional information
This work was supported by grants FUNCUNESP, FAPESP and CNPq.
The authors are grateful to Mr Renato Devidé for technical assistance.
Rights and permissions
About this article
Cite this article
Foresti, F., Oliveira, C. & Foresti de Almeida-Toledo, L. A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine. Experientia 49, 810–813 (1993). https://doi.org/10.1007/BF01923555
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01923555