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A recombination survey using microsatellites: the O chromosome of Drosophila subobscura

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Abstract

Recombination plays an important role in species adaptation since it acts as an evolutionary force that can influence genome pattern organization. However, recombination can be detrimental in some situations, causing the breakdown of some adaptive gene combinations such as coadapted gene complexes. Genetic and cytological chromosome maps allow recombination throughout the genome to be analyzed. In this study we compare the recombination rate of two types of homokaryotypic lines of D. subobscura (OST and O 3+4 ) using a set of at least 13 microsatellite loci. The genetic maps obtained present similar lengths: 184 and 196 cM for OST and O 3+4 chromosomes, respectively. For most pairs of markers analyzed, a sample size of about 150 individuals appeared sufficient to obtain appropriate recombination values, with the exception of markers located in the same cytological band. Recombination rates seemed to be fairly uniform along the O chromosome, but some regional differences were observed. Several recombination hot and coldspots were detected, and their numbers were different in the homokaryotypic line types (OST and O 3+4 ). This variability could be attributed to differences between the genetic content of the two arrangements or to differences between the lines.

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Acknowledgments

We would like to thank Prof. Joan Balanyà (Dept. of Genetics, Universitat de Barcelona) for the analyses of the polytene chromosomes, Prof. Concepció Arenas (Dept. of Statistics Universitat de Barcelona) for her statistical advice and Mr. Robin Rycroft (Language Advisory Service, Universitat de Barcelona) for the English grammar corrections. Furthermore, we would also like to thank Prof. Charles F. Aquadro, Prof. Martha Hamblin (Cornell University), Prof. Luís Serra and Prof. Enrique Blanco (Universitat de Barcelona) for their valuable comments on the manuscript. This work was supported by a pre-doctoral fellowship to CP (2009FIC-00096) from the Generalitat de Catalunya (Spain). The research was funded by projects CGL2006-13423-C02-02 from the Ministerio de Ciencia y Tecnología (MCYT, Spain) and SGR2009-636 from the Generalitat de Catalunya (Spain).

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  1. Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Avd. Diagonal 645, 08028, Barcelona, Spain

    Cinta Pegueroles, Pedro A. Araúz, Marta Pascual & Francesc Mestres

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  1. Cinta Pegueroles
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  2. Pedro A. Araúz
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  3. Marta Pascual
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  4. Francesc Mestres
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Correspondence to Cinta Pegueroles.

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Pegueroles, C., Araúz, P.A., Pascual, M. et al. A recombination survey using microsatellites: the O chromosome of Drosophila subobscura . Genetica 138, 795–804 (2010). https://doi.org/10.1007/s10709-010-9461-0

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  • DOI: https://doi.org/10.1007/s10709-010-9461-0

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