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Plastid DNA variation in highly fragmented populations of Microbiota decussata Kom. (Cupressaceae), an endemic to Sikhote Alin Mountains

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Abstract

Microbiota decussata Kom. (Cupressaceae) is a subalpine species endemic to the Sikhote Alin Mountains with populations scattered throughout the range. We used sequence data for four noncoding regions of chloroplast DNA to characterize the genetic diversity in populations sampled from different parts of M. decussata natural range. No variation was observed in the trnT–trnF region, whereas the trnH–psbA, trnS–trnfM, and trnS–trnG regions showed polymorphisms. At the species level, we found a low nucleotide diversity (π = 0.0009) and high haplotype diversity (h = 0.981) as well as high differentiation (ΦST = 0.420). NST and GST values suggested the existence of a phylogeographic structure in M. decussata. The observed patterns of diversity could be explained in part by ecological features of the species and its long-term persistence throughout the range with population expansion, successive fragmentation and isolation.

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Acknowledgements

We are grateful to Dr. Alla B. Melnikova and Dr. Valentin V. Yakubov for their assistance in collecting plant material. We also appreciate the helpful comments on this manuscript from two anonymous referees. This work was supported by FEBRAS grants 06-I-P11-028, 06-I-P11-030 and 06-II-CO-05-021; and by grants NSH 6923-2006.4 and NSH 8969-2006.4 “Leading School of Thought” from the President of Russian Federation.

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  1. Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 690022, Vladivostok, Russia

    Elena V. Artyukova, Marina M. Kozyrenko & Yury N. Zhuravlev

  2. Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, 690950, Vladivostok, Russia

    Peter G. Gorovoy

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  1. Elena V. Artyukova
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  2. Marina M. Kozyrenko
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  3. Peter G. Gorovoy
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Correspondence to Elena V. Artyukova.

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Artyukova, E.V., Kozyrenko, M.M., Gorovoy, P.G. et al. Plastid DNA variation in highly fragmented populations of Microbiota decussata Kom. (Cupressaceae), an endemic to Sikhote Alin Mountains. Genetica 137, 201–212 (2009). https://doi.org/10.1007/s10709-009-9386-7

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