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Genetic differentiation among Greek lake populations of Carassius gibelio and Cyprinus carpio carpio

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Abstract

The genetic structure of the Western Greece lake populations of Carassius gibelio and Cyprinus carpio carpio populations was characterized by using a PCR-based RFLP and sequencing analysis of mitochondrial rDNA genes and regions (16S rDNA, cytochrome b and D-loop). Our analysis was able to detect: (a) two haplotypes in C. c. carpio populations and two haplotypes in C. gibelio populations (b) a high nucleotide divergence between the two species and (c) two genetically distinct C. gibelio populations, one existing in the Amvrakia habitat (AMV1) with a second in Ozeros and Trichonida (OZE1 and TRI1) habitat. The present analysis indicates that genetic diversity observed was limited with a haplotype index between 0.0 and 55.6%, and a nucleotide diversity within and among populations between 0.0 and 1.27%. It also underlines a restricted mtDNA-based evaluation of the phylogenetic relationships among C. gibelio and C. c. carpio populations. In addition, the present study contributed knowledge on the genetic variation and structure of these populations which is absolutely necessary for any efficient fish management and/or conservation programme.

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Abbreviations

cyt b :

Cytochrome b

mtDNA:

Mitochondrial DNA

NJ:

Neighbor joining

MP:

Maximum parsimony

PCR:

Polymerase chain reaction

TS:

Transitions

TV:

Transversions

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Acknowledgments

The authors would like to thank Dr. G. Kilias and Dr. V. Papasotiropoulos for their help and fruitful comments on the manuscript. This work was supported in part by intramural funding provided by the Technological Educational Institution (T.E.I.) of Messolonghi (Project 2/16-10-03 from Research Committee) and the University of Ioannina, Greece. The authors would like to thank Prof. William J. Etges and an anonymous reviewer for valuable comments on the manuscript.

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Authors and Affiliations

  1. Department of Environmental and Natural Resources Management, University of Ioannina, Agrinio, 30100, Greece

    George Tsipas, George Tsiamis & Kostas Bourtzis

  2. Laboratory of Ichthyology, Department of Aquaculture and Fisheries, Technological Educational Institution (T.E.I.) of Messolonghi, Messolonghi, 30200, Greece

    George Tsipas & Kosmas Vidalis

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  1. George Tsipas
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Correspondence to Kostas Bourtzis.

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Tsipas, G., Tsiamis, G., Vidalis, K. et al. Genetic differentiation among Greek lake populations of Carassius gibelio and Cyprinus carpio carpio . Genetica 136, 491–500 (2009). https://doi.org/10.1007/s10709-008-9331-1

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