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Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites

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Abstract

The cultivated diploid, Gossypium arboreum L., (A genome) is an invaluable genetic resource for improving modern tetraploid cotton (G. hirsutum L. and G. barbadense L.) cultivars. The objective of this research is to select a set of informative and robust microsatellites for studying genetic relationships among accessions of geographically diverse G. arboreum cultivars. From more than 1,500 previously developed simple sequence repeat (SSR) markers, 115 genomic (BNL) and EST-derived (MUCS and MUSS) markers were used to evaluate the allelic diversity of a core panel of G. arboreum accessions. These SSR data enabled advanced genome analyses. A set of 25 SSRs were selected based both upon their high level of informativeness (PIC ≥ 0.50) and the production of clear PCR bands on agarose gels. Subsequently, 96 accessions representing a wide spectrum of diversity of G. arboreum cultivars were analyzed with these markers. The 25 SSR loci revealed 75 allelic variants (polymorphisms) ranging from 2 to 4 alleles per locus. The Neighborjoining (NJ) method, based on genetic dissimilarities, revealed that cultivars from geographically adjacent countries tend to cluster together. Outcomes of this research should be useful in decreasing redundancy of effort and in constructing a core collection of G. arboreum, important for efficient use of this genetic resource in cotton breeding.

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Acknowledgement

We thank the Delta & Pine Land Company for financial support.

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Authors and Affiliations

  1. Southern Illinois University, Plant Soil and Agricultural Systems, Carbondale, IL, 62901, USA

    Stella K. Kantartzi

  2. USDA-ARS, Western Integrated Cropping Systems, Shafter, CA, 93263, USA

    Mauricio Ulloa

  3. USDA-ARS, Stoneville, MS, 38776, USA

    Erik Sacks

  4. Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701, USA

    James McD. Stewart

Authors
  1. Stella K. Kantartzi
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  2. Mauricio Ulloa
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  3. Erik Sacks
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  4. James McD. Stewart
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Correspondence to Stella K. Kantartzi.

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Kantartzi, S.K., Ulloa, M., Sacks, E. et al. Assessing genetic diversity in Gossypium arboreum L. cultivars using genomic and EST-derived microsatellites. Genetica 136, 141–147 (2009). https://doi.org/10.1007/s10709-008-9327-x

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  • DOI: https://doi.org/10.1007/s10709-008-9327-x

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