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Identification and detection of genetic relatedness among important varieties of pea (Pisum sativum L.) grown in India

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Abstract

Among the cool season legume crops grown in India and the Indian sub-continent, peas are very popular and preferred by the growers as well as consumers for various uses. The third largest area in pea cultivation is occupied by India after Canada and Russia. Among the important and popular varieties of peas that are grown in India, several are from exotic background. But very little work has been done to carry out the genetic diversity present in the widely adapted Indian pea varieties using DNA markers. Twenty-four most popular and widely adapted varieties were subjected to RAPD analysis to find out the genetic relatedness among them using 60 decamer primers. All the primers used in our study were found to be polymorphic and seven of them showed 100% polymorphism. Out of 579 amplified products, 433 showed polymorphism (74.8%). On an average, 9.65 bands were amplified per primer. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped all the tall type varieties together, whereas, dwarf types formed two different clusters based upon their pedigree. The arithmetic mean heterozygosity (H av) value and marker index (MI) was found to be 0.496 and 4.787, respectively, thus this indicated the efficiency of RAPD as a marker system. Moreover, the calculated value of probability of identical match by chance suggested that about 1053 genotypes can be unambiguously distinguish by employing 60 RAPD primers.

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Acknowledgements

Authors are thankful to Dr. Masood Ali, Director, Indian Institute of Pulses Research, Kanpur, India for his consistent encouragement and guidance. Authors are also thankful to Dr. Naimuddin, Scientist, IIPR, Kanpur for providing help during the study.

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  1. Biotechnology Unit, Indian Institute of Pulses Research, Type 4/9, IIPR Campus, Kalyanpur, Kanpur, Uttar Pradesh, 208 024, India

    P. Ray Choudhury, Hasan Tanveer & G. P. Dixit

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  1. P. Ray Choudhury
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  2. Hasan Tanveer
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  3. G. P. Dixit
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Correspondence to P. Ray Choudhury.

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Choudhury, P.R., Tanveer, H. & Dixit, G.P. Identification and detection of genetic relatedness among important varieties of pea (Pisum sativum L.) grown in India. Genetica 130, 183–191 (2007). https://doi.org/10.1007/s10709-006-9005-9

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  • DOI: https://doi.org/10.1007/s10709-006-9005-9

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