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Characterization of the satellite DNA Msat-160 from the species Chionomys nivalis (Rodentia, Arvicolinae)

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Abstract

The satellite DNA Msat-160 has been previously characterized in several species of the genus Microtus. Here we present the characterization of Msat-160 from Chionomys nivalis, a species with a very primitive karyotype. As in other Microtus species analyzed, C. nivalis Msat-160 is AT rich, has a monomer length of 160 bp, is undermethylated and is mainly located in all the pericentromeric heterochromatin of all autosomes and the X chromosome, but is completely absent from the Y chromosome. Hence, our results support the hypothesis that Msat-160 was initially distributed in the pericentromeric heterochromatin of all autosomes and the X chromosome. The taxonomic status of the genus Chionomys in relation to the genus Microtus is a very interesting issue, so we constructed phylogenetic dendrograms using Msat-160 sequences from several Microtus species. Although the results were not informative about this issue, the presence of Msat-160 in C. nivalis and Microtus species suggested that both genera are closely related and that this satellite DNA was present in the common ancestor. Studies of Msat-160 in different arvicoline species could help to determine the origin of this satellite and, perhaps, to establish the phylogenetic relationships of some arvicoline groups.

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Acknowledgments

The authors wish to thanks Parque Nacional de Sierra Nevada (Granada, Spain) for the permission to capture C. nivalis. This work was supported by the Spanish Ministerio de Ciencia y Tecnología through project numbers: BOS2002-04150-C02-01 and BOS2002-04150-C02-02 and by the Junta de Andalucía throughout the programme “Ayudas a grupos de investigación”, group numbers: CVI 0109 and CVI 220.

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Authors and Affiliations

  1. Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Campus Las Lagunillas s/n, E-23071, Jaén, Spain

    M. J. Acosta, J. A. Marchal, S. Martínez, E. Puerma, M. Bullejos & A. Sánchez

  2. Departamento de Genética, Facultad de Ciencias, Universidad de Granada, E-18071, Granada, Spain

    R. Díaz la de Guardia

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  1. M. J. Acosta
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  2. J. A. Marchal
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  3. S. Martínez
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  4. E. Puerma
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  5. M. Bullejos
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  6. R. Díaz la de Guardia
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  7. A. Sánchez
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Correspondence to A. Sánchez.

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Acosta, M.J., Marchal, J.A., Martínez, S. et al. Characterization of the satellite DNA Msat-160 from the species Chionomys nivalis (Rodentia, Arvicolinae). Genetica 130, 43–51 (2007). https://doi.org/10.1007/s10709-006-0018-1

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  • DOI: https://doi.org/10.1007/s10709-006-0018-1

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