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Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

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Abstract

Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.

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Authors and Affiliations

  1. Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65, Brno, Czech Republic

    Eduard Kejnovsky, Zdenek Kubat, Roman Hobza, Martina Lengerova & Boris Vyskot

  2. Kazusa DNA Research Institute, Kisarazu, 292-0818, Chiba, Japan

    Shusei Sato & Satoshi Tabata

  3. Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, 565-0871, Osaka, Japan

    Kiichi Fukui & Sachihiro Matsunaga

Authors
  1. Eduard Kejnovsky
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  2. Zdenek Kubat
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  3. Roman Hobza
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  4. Martina Lengerova
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  5. Shusei Sato
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  6. Satoshi Tabata
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  7. Kiichi Fukui
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  8. Sachihiro Matsunaga
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  9. Boris Vyskot
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Correspondence to Eduard Kejnovsky.

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Kejnovsky, E., Kubat, Z., Hobza, R. et al. Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia . Genetica 128, 167–175 (2006). https://doi.org/10.1007/s10709-005-5701-0

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  • DOI: https://doi.org/10.1007/s10709-005-5701-0

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