Summary
5S rDNA sequences present an intense dynamism and have proved to be valuable as genetic markers to distinguish closed related species and also in the understanding of the evolutionary dynamic of repetitive sequences in the genomes. In order to identify patterns of 5S rDNA organization and their evolution in the genome of fish species, such genomic segment was investigated in the tilapias Oreochromis niloticus and Tilapia rendalli, and in the hybrid O. urolepis hornorum × O. mossambicus. A dual 5S rDNA system was identified in the three analyzed tilapia samples. Although each 5S rDNA class was conserved among the three samples, a distinct 5S rDNA genome organization pattern could be evidenced for each sample. The presence of a dual 5S rDNA system seems to be a general trait among non-related teleost fish orders, suggesting that evolutionary events of duplication have occurred before the divergence of the main groups of teleost fishes.
Similar content being viewed by others
References
S.F. Altschul W. Gish W. Miller E.W. Myers D.J. Lipman (1990) ArticleTitleBasic local alignment search tool J. Mol. Biol. 215 403–410 Occurrence Handle2231712 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D Occurrence Handle10.1006/jmbi.1990.9999
V. Amarasinghe J.E. Carlson (1998) ArticleTitlePhysical mapping and characterization of 5S rRNA genes in gouglas-fir Am. Gen. Assoc. 89 495–500 Occurrence Handle1:CAS:528:DyaK1MXjsVWjsQ%3D%3D
W.J. Baker T.A. Hedderson J. Dransfield (2000) ArticleTitleMolecular phylogenetics of Calamus (Palmae) and related rattan genera based on 5S rDNA spacer sequence data Mol. Phyl. Evol. 14 218–231 Occurrence Handle1:CAS:528:DC%2BD3cXhtFGjsrY%3D Occurrence Handle10.1006/mpev.1999.0697
F. Bardakci D.O.F. Skibinski (1994) ArticleTitleApplication of the RAPD technique in tilapia fish: species and subspecies identification Heredity 73 117–123 Occurrence Handle8071079 Occurrence Handle1:CAS:528:DyaK2MXhtl2ht7c%3D
B.R. Baum L.G. Bailey (1997) ArticleTitleThe molecular diversity of the 5S rRNA gene in Kengyilia alatavica (Drobov) J. L. Yang, Yen & Baum (Poaceae: Triticeae): potential genomic assignment of different rDNA units Genome 40 215–228 Occurrence Handle9134703 Occurrence Handle1:CAS:528:DyaK2sXjt1aru70%3D Occurrence Handle10.1006/geno.1996.4535
A. Belkhiri J. Buchko G.R. Klassen (1992) ArticleTitleThe ribosomal RNA gene in Pythium species: two different genomic locations Mol. Biol. Evol. 9 1089–1102 Occurrence Handle1435236 Occurrence Handle1:CAS:528:DyaK3sXltFSitbw%3D
R.C. Cronn X. Zhao A.H. Paterson J.F. Wendel (1996) ArticleTitlePolymorphism and concerted evolution in a tandemly repeated gene family: 5S ribosomal DNA in diploid and allopolyploid cottons J. Mol. Evol. 42 685–705 Occurrence Handle8662014 Occurrence Handle1:CAS:528:DyaK28Xkt1Omsbo%3D Occurrence Handle10.1007/BF02338802
A.M. Deiana A. Cau S. Salvadori E. Coluccia R. Cannas A. Milia J. Tagliavini (2000) ArticleTitleMajor and 5S ribosomal␣sequences of the largemouth bass Micropterus salmoides Perciformes, Centrarchidae are localized in GC-rich regions of the genome Chromosome Res. 8 213–218 Occurrence Handle10841048 Occurrence Handle1:CAS:528:DC%2BD3cXjvVSjsrg%3D Occurrence Handle10.1023/A:1009248828365
G. Drouin M. Moniz Sá Particlede (1995) ArticleTitleThe concerted evolution of 5S ribosomal genes linked to the repeat units of other multigene families Mol. Biol. Evol. 12 481–493 Occurrence Handle7739390 Occurrence Handle1:CAS:528:DyaK2MXltF2nur4%3D
J. Felsenstein (1985) ArticleTitleConfidence limits on phylogenies: an approach using the bootstrap Evolution 39 783–791 Occurrence Handle10.2307/2408678
S. Frederiksen H. Cao B. Lomholt G. Levan C. Hallemberg (1997) ArticleTitleThe rat 5S rRNA bona fide gene repeat maps to chromosome 19q12→qter and the pseudogene repeat maps to 12q12 Cytogenet. Cell Genet. 76 101–106 Occurrence Handle9154136 Occurrence Handle1:CAS:528:DyaK2sXjsVSms74%3D
F. Galls J.A.J. Metz (1998) ArticleTitleWhy are there so many cichlid species? Trends Ecol. Evol. 13 1–2 Occurrence Handle10.1016/S0169-5347(97)01239-1
E. Gornung S. Innocentiis ParticleDe F. Annesi L. Sola (2000) ArticleTitleZebrafish 5S rRNA genes map to the long arms of chromosome 3 Chromosome Res. 8 362 Occurrence Handle10919729 Occurrence Handle1:CAS:528:DC%2BD3cXltlShuro%3D Occurrence Handle10.1023/A:1009252017097
P.H. Greenwood (1991) Speciation M.H.A. Keenleyside (Eds) Cichlid Fishes: Behavior, Ecology and Evolution Chapman & Hall New York 86–102
C. Hallemberg J. Nederby-Nielsen S. Frederiksen (1994) ArticleTitleCharacterization of 5S rRNA genes from mouse Gene 142 291–295 Occurrence Handle10.1016/0378-1119(94)90277-1
R.E. Hanson M.N. Islam-Faridi E.A. Percival C.F. Crane Y. Ji T.D. McKnight D.M. Stelly H.J. Price (1996) ArticleTitleDistribution of 5S and 18–28S rDNA loci in a tetraploid cotton (Gossypium hirsutum L.) and its putative diploid ancestors Chromosoma 105 55–61 Occurrence Handle8662259 Occurrence Handle1:CAS:528:DyaK28XlslKlsLw%3D Occurrence Handle10.1007/s004120050159
M. Kimura (1980) ArticleTitleA simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences J. Mol. Evol. 16 111–120 Occurrence Handle7463489 Occurrence Handle1:CAS:528:DyaL3MXmtFSktg%3D%3D Occurrence Handle10.1007/BF01731581
H. Komiya S. Takemura (1979) ArticleTitleNucleotide sequence of 5S ribosomal RNA from rainbow trout (Salmo gairdnerii) liver J. Biochem. 100 369–374
H. Komiya M. Hasegawa (1986) ArticleTitleDifferentiation of oocyte – and somatic – type 5S rRNAs in animals J. Biochem. 100 369–374 Occurrence Handle3782056 Occurrence Handle1:CAS:528:DyaL28XlsFSiu74%3D
S. Kumar K. Tamura I.B. Jackobsen M. Nei (2001) ArticleTitleMEGA2: molecular evolutionary genetics analysis sofware Bioinformatics 17 1244–1245 Occurrence Handle11751241 Occurrence Handle1:CAS:528:DC%2BD38XmtVCktQ%3D%3D Occurrence Handle10.1093/bioinformatics/17.12.1244
R. Leah S. Frederiksen J. Engberg P.D. Sorensen (1990) ArticleTitleNucleotide sequence of a mouse 5S rRNA variant gene Nucleic Acids Res. 18 7441 Occurrence Handle2259634 Occurrence Handle1:CAS:528:DyaK3MXhsFyhtL4%3D
L.L. Lovshin (2000) Tilapia culture in Brazil A. Costa-Pierce J.E. Rakocy (Eds) A Tilapia culture in the Americas The World Aquaculture Society Baton Rouge, Louisiana 133–140
C. Martins A.P. Wasko C. Oliveira J.M. Wright (2000) ArticleTitleNucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the tilapiine cichlid fish, Oreochromis niloticus Hereditas 133 39–46 Occurrence Handle11206852 Occurrence Handle1:CAS:528:DC%2BD3MXht1Srt7w%3D Occurrence Handle10.1111/j.1601-5223.2000.00039.x
C. Martins P.M. Galetti SuffixJr. (2001a) ArticleTitleOrganization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct non-transcribed spacers Genome 44 903–910 Occurrence Handle1:CAS:528:DC%2BD3MXos12ls7k%3D Occurrence Handle10.1139/gen-44-5-903
C. Martins P.M. Galetti SuffixJr. (2001b) ArticleTitleTwo 5S rDNA arrays in Neotropical fish species: is it a general rule for fishes? Genetica 111 439–446 Occurrence Handle1:CAS:528:DC%2BD38XhsVKqtbY%3D Occurrence Handle10.1023/A:1013799516717
C. Martins A.P. Wasko (2004) Organization and evolution of 5S ribosomal DNA in the fish genome C.R. Williams (Eds) Focus on Genome Research Nova Science Publishers Hauppauge, NY 289–318
C. Martins A.P. Wasko C. Oliveira F. Porto-Foresti P.P. Parise-Maltempi J. Wright F. Foresti (2002) ArticleTitleDynamics of 5S rDNA in the tilápia (Oreochromis niloticus) genome: repeat units, inverted sequences, pseudogenes and chromosome loci Cytogenet. Genome Res. 98 78–85 Occurrence Handle12584446 Occurrence Handle1:CAS:528:DC%2BD3sXitVygtrY%3D Occurrence Handle10.1159/000068542
P. Morán J.L. Martínez E. Garcia-Vásquez A.M. Pendás (1996) ArticleTitleSex linkage of 5S rDNA in rainbow trout (Oncorhynchus mykiss) Cytogenet. Cell Genet. 75 145–150 Occurrence Handle9040781
K. Ota Y. Tateno T. Gojobori (2003) ArticleTitleHighly differentiated and conserved sex chromosome in fish species (Aulopus japonicus: Teleostei, Aulopidae) Gene 317 187–193 Occurrence Handle14604807 Occurrence Handle1:CAS:528:DC%2BD3sXosFyis7o%3D Occurrence Handle10.1016/S0378-1119(03)00702-9
A.M. Pendás P. Móran J.P. Freije E. Garcia-Vásquez (1994) ArticleTitleChromosomal location and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA Cytogenet. Cell Genet. 67 31–36 Occurrence Handle8187548
A.M. Pendás P. Morán J.L. Martínez E. Garcia-Vazquez (1995) ArticleTitleApplications of 5S rDNA in Atlantic salmon, brown trout, and in Atlantic salmon × brown trout hybrid Mol. Ecol. 4 275–276 Occurrence Handle7735532
E.M. Pino Particledel C. Murphy P.H. Masson J.G. Gall (1992) ArticleTitle5S rRNA-encoding genes of the marsupial frog Gastrotheca riobambae Gene 111 235–238 Occurrence Handle1541401 Occurrence Handle10.1016/0378-1119(92)90692-I
R.S.V. Pullin (1991) Cichlids in aquaculture M.H.A. Keenleyside (Eds) Cichlid Fishes: Behavior, Ecology and Evolution Chapman & Hall New York 280–309
F. Robles R. la Herran ParticleDe A. Ludwig C.R. Rejon M.R. Rejon M.A. Garrido-Ramos (2005) ArticleTitleGenomic organization and evolution of the 5S ribosomal DNA in the ancient fish sturgeon Genome 48 18–28 Occurrence Handle15729393 Occurrence Handle1:CAS:528:DC%2BD2MXjs1WmtbY%3D Occurrence Handle10.1139/g04-077
S.L. Sadjak K.M. Reed R.B. Phillips (1998) ArticleTitleIntraindividual and interspecies variation in the 5S rDNA of coregonid fish J. Mol. Evol. 46 680–688 Occurrence Handle10.1007/PL00006348
J. Sambrook D.W. Russel (2001) Molecular Cloning. A Laboratory Manual. Third Edition Cold Spring Harbor Laboratory Press New York
H. Suzuki K. Moriwaki S. Sakurai (1994) ArticleTitleSequences and evolutionary analysis of mouse 5S rDNAs Mol. Biol. Evol. 11 704–710 Occurrence Handle8078409 Occurrence Handle1:CAS:528:DyaK2cXlt1Kmtb4%3D
H. Suzuki S. Sakurai Y. Matsuda (1996) ArticleTitleRat 5S rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19 Cytogenet. Cell Genet. 72 1–4 Occurrence Handle8565624 Occurrence Handle1:CAS:528:DyaK28XhsFKjsL4%3D Occurrence Handle10.1159/000134149
J.D. Thompson D.G. Higgins T.J. Gibson (1994) ArticleTitleClustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res. 22 4673–4680 Occurrence Handle7984417 Occurrence Handle1:CAS:528:DyaK2MXitlSgu74%3D
B. Venkatesh (2003) ArticleTitleEvolution and diversity of fish genomes Curr. Opin. Genet. Develop. 13 588–592 Occurrence Handle1:CAS:528:DC%2BD3sXpt1SnsLg%3D Occurrence Handle10.1016/j.gde.2003.09.001
A.P. Wasko C. Martins J.M. Wright P.M. Galetti SuffixJr. (2001) ArticleTitleMolecular organization of 5S rDNA in fishes of the genus Brycon Genome 44 893–902 Occurrence Handle11681614 Occurrence Handle1:CAS:528:DC%2BD3MXos12ls7g%3D Occurrence Handle10.1139/gen-44-5-893
A.P. Wasko C. Martins C. Oliveira F. Foresti (2003) ArticleTitleNon-destrutive genetic sampling in fish: an improved method for DNA extraction from fish fins and scales Hereditas 138 161–165 Occurrence Handle14641478 Occurrence Handle10.1034/j.1601-5223.2003.01503.x
Zimmermann, S., 1999. Incubação Artificial: técnica que permite a produção de tilápias do Nilo geneticamente superiores. Panor. Aqüic. mar./abr.: 69
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Alves-Costa, F.A., Wasko, A.P., Oliveira, C. et al. Genomic organization and evolution of the 5S ribosomal DNA in Tilapiini fishes. Genetica 127, 243–252 (2006). https://doi.org/10.1007/s10709-005-4013-8
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10709-005-4013-8