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Atlantic cod (Gadus morhua) larvae can biosynthesis phospholipid de novo from 2-oleoyl-glycerol and glycerol precursors

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Abstract

The dietary requirement of phospholipid (PL) of fish larvae has been suggested to originate in an inefficient ability for de novo biosynthesis of PL based on dietary triacylglycerol (TAG). The main objective of the present study was to investigate whether cod larvae could synthesis PL from sn-2-monoacylglycerol (2-MAG) and glycerol precursors. A tube feeding method was used to deliver equal molar aliquots of 2-oleoyl-[1,2,3-3H]glycerol and [U-14C] glycerol together with bovine serum albumin (BSA) bound 16:0 (palmitic acid) and 22:6n-3 (docosahexaenoic acid, DHA), with or without choline chloride to the foregut of anesthetized cod larvae and thereafter monitoring the metabolism of these components in the larvae through 4 h following injection. Our results showed that both 2-MAG and glycerol precursors contributed to the de novo synthesis of phosphatidylcholine (PC) and the 2-MAG pathway predominated over the G-3-P (glycerol-3-phosphate) pathway in the synthesis of TAG and PC. The molecular ratio of PC/TAG obtained from the 2-MAG and the G-3-P pathways was 0.44–0.74 and 1.02–2.06 within the first hour of tube feeding, suggesting they might have comparable biosynthesis ability of PC and TAG under the conditions of the present study. Furthermore, supplementation of choline chloride significantly increased PC/TAG ratio (p < 0.05) for both pathways. However, further studies are needed to quantify the enzyme activity involved in the CDP-choline (cytidine diphosphate choline) pathway, and the function of choline either in simulating PC synthesis or TAG catabolism or both needs further investigation.

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Acknowledgments

Thanks to Dr. Peter De Schryver for his generous donation of the cod larvae. We thank the Department of Biology at NTNU for providing financial support to Keshuai Li for his PhD study (N31114).

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Authors and Affiliations

  1. Department of Biology, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway

    Keshuai Li, Rolf Erik Olsen, Mari-Ann Østensen, Elin Kjørsvik & Yngvar Olsen

  2. BioTrix, NO-7022, Trondheim, Norway

    Dag Altin

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  1. Keshuai Li
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  2. Rolf Erik Olsen
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Correspondence to Keshuai Li.

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Li, K., Olsen, R.E., Østensen, MA. et al. Atlantic cod (Gadus morhua) larvae can biosynthesis phospholipid de novo from 2-oleoyl-glycerol and glycerol precursors. Fish Physiol Biochem 42, 137–147 (2016). https://doi.org/10.1007/s10695-015-0125-y

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