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Protein-sparing effect of dietary lipid in practical diets for blunt snout bream (Megalobrama amblycephala) fingerlings: effects on digestive and metabolic responses

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Abstract

This study aimed to evaluate the protein-sparing effect of dietary lipid on digestive and metabolic responses of fingerling Megalobrama amblycephala. Fish were fed nine practical diets with three protein levels (270, 310 and 350 g kg−1) and three lipid levels (40, 70 and 100 g kg−1) for 8 weeks. Weight gain was significantly affected only by dietary lipid levels with the highest found in fish fed 70 g kg−1 lipid. Relative feed intake and whole-body protein content showed little difference among all the treatments. Activities of intestine lipase and amylase increased significantly as dietary lipid levels increased, whereas little difference was observed in protease activities. Liver lipid content was significantly affected only by protein levels with the lowest found in fish fed 310 g kg−1 protein. Liver aspartate aminotransferase (GOT) activities increased significantly with decreasing lipid levels, whereas the highest GOT activity was obtained in fish fed 310 g kg−1 protein in terms of dietary protein levels. Activities of liver lipoprotein lipase, total lipase and plasma cholesterol concentration of fish fed 350 g kg−1 protein were significantly lower than that of the other groups, whereas the same was true for plasma 3, 5, 3′-triiodothyronine level of fish fed 270 g kg−1 protein. The results indicated that an increase of dietary lipid content from 40 to 70 g kg−1 can enhance the growth and digestive enzyme activities of this species and reduce the proportion of dietary protein catabolized for energy without inducing hepatic steatosis; meanwhile, decreasing protein level from 350 to 310 g kg−1 leads to the increase of lipase activities both in intestine and liver coupled with the reduced liver lipid content.

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Acknowledgments

This research was supported by the funds of National Technology System for Conventional Freshwater Fish Industries of China (nycytx-49-21). We thank the Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences, for use of their facilities and donation of the bream. The authors also are grateful to Hao Zhu, Guang-zhen Jiang, Kang-le Lu and Gui-feng Li for their assistance with sample preparation and analysis.

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Authors and Affiliations

  1. Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Xiangfei Li, Yangyang Jiang & Wenbin Liu

  2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu Province, People’s Republic of China

    Xianping Ge

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  1. Xiangfei Li
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  2. Yangyang Jiang
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Correspondence to Wenbin Liu.

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Li, X., Jiang, Y., Liu, W. et al. Protein-sparing effect of dietary lipid in practical diets for blunt snout bream (Megalobrama amblycephala) fingerlings: effects on digestive and metabolic responses. Fish Physiol Biochem 38, 529–541 (2012). https://doi.org/10.1007/s10695-011-9533-9

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