Abstract
The levels of metallothionein (MT), a biomarker of metal exposure, and of Cd and Cu, known as MT inducers, were investigated in Sparus aurata intraperitoneally injected with 500 μg/kg of Cu and Cd for 2 days. MT and metal concentrations (Cd and Cu) were determined in liver, gills and kidney. MT levels were significantly increased in all investigated tissues, with the highest value in liver of Cu as Cd-treated fishes (3.56-fold and 3.3- fold, respectively). Metal concentrations were statistically different between all tissues. Highest metal concentrations were in the liver. The higher metal concentrations and MT induction levels support the main role of MT in metal homeostasis and detoxification.
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Alhama J, Romero-Ruiz A, López-Barea J (2006) Metallothionein quantification in clams by reversed-phase high-performance liquid chromatography coupled to fluorescence detection after monobromobimane derivatization. J Chromatogr A 1107:52–58
Amiard JC, Pineau A, Boiteau H-L, Metayer C, Amiard-Triquet C (1987) Application de la spectrométrie d’absorption atomique Zeeman aux dosages de huit éléments traces (Ag, Cd, Cr, Cu, Mn, Ni, Pb et Se) dans les matrices biologiques solides. Water Res 21:693–697
Andrews GK (2000) Regulation of metallothionein gene expression by oxidative stress and metal ions. Biochem Pharmacol 59:95–104
Banni M, Jebali J, Daubeze M, Clerandau C, Guerbej H, Narbonne JF, Boussetta H (2005) Monitoring pollution in Tunisian coasts: application of a classification scale based on biochemical markers. Biomarkers 10(2–3):105–116
Banni M, Dondero F, Jebali J, Guerbej H, Boussetta H, Viarengo A (2007) Assessment of heavy metal contamination using real time PCR analysis of mussel metallothionein mt10 and mt20 expression: a validation along the Tunisian coasts. Biomarkers 12(4):369–383
Bebianno MJ, Machado LJ (1997) Concentrations of metals and metallothioneins in Mytilus galloprovincialis along the south coast of Portugal. Mar Pollut Bull 34(8):666–671
Bermejo-Nogales A, Saera-Vila A, Calduch-Giner JA, Navarro J, Sitjă-Bobadilla A, Pėrez-Sănchez J (2007) Differential metabolic and gene expression profile of juvenile common dentex (Dentex dentex L.) and gilthead sea bream (Sparus aurata L.) in relation to redox homeostasis. Aquaculture 267:213–224
Bourdineaud JP, Baudrimont M, Gonzalez P, Moreau JL (2006) Challenging the model for induction of metallothionein gene expression. Biochimie 88:1787–1792
Bunton TE, Baksi SM, George SG, Frazier JM (1987) Abnormal hepatic copper storage in a teleost fish (Morone americana). Vet Pathol 24:515–524
Bustamante P, Morales CF, Mikkelsen B, Dam M, Caurant F (2004) Trace element bioaccumulation in grey seals Halichoerus grypus from the Faroe islands. Mar Ecol Prog Ser 267:291–301
Castano A, Carbonell G, Carballo M, Fernandez C, Boleas S, Tarazona JV (1996) Sublethal effects of repeated intraperitoneal cadmium injections on rainbow trout (Oncorhynchus mykiss). Ecotoxicol Environ Saf 41:29–35
Costa PM, Repolho T, Caeiro S, Diniz ME, Moura I, Costa MH (2007) Modelling metallothionein induction in the liver of Sparus aurata exposed to metal-contaminated sediments. Ecotoxicol Environ Saf 71:117–124
Dallinger R, Egg M, Köck G, Hofer R (1997) The role of metallothionein cadmium accumulation of arctic char (Salvelinus alpinus) from high alpine lakes. Aquat Toxicol 38:47–66
De Boeck G, Huong-Ngo TT, Van Campenhout K, Blust R (2003) Differential metallothionein induction patterns in three freshwater fish during sublethal copper exposure. Aquat Toxicol 65:413–424
De Smet H, De Wachter B, Lobinski R, Blust R (2001) Dynamics of (Cd, Zn)-metallothioneins in gills, live rand kidney of common carp Cyprinus carpio during cadmium exposure. Aquat Toxicol 52:269–281
Dondero F, Piacentini L, Banni M, Rebelo M, Burlando B, Viarengo A (2005) Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation. Gene 345:259–270
Filipovic V, Raspor B (2003) Metallothioneinn and metal levels in cytosol of liver, kidney and brain in relation to growth parameters of Mullus surmuletus and Liza aurata from the eastern Adriatic Sea. Water Res 37:3253–3262
George SG, Todd K, Wright J (1996) Regulation of metallothionein in teleosts: induction of MTm RNA and protein by cadmium in hepatic and extrahepatic tissues of a marine flatfish, the turbot (Scophthalmus maximus). Comp Biochem Physiol 113C(2):109–115
Giguére A, Campbell PGC, Hare L, McDonald DG, Rasmussen JB (2004) Influence of lake chemistry and fish age on Cd, Cu and Zn concentrations in various organs of indigenous yellow perch (Perca flavescens). Can J Fish Aquat Sci 61:1702–1716
Hamilton SJ, Mehrle PM (1986) Metallothionein in fish: review of its importance in assessing stress from metal contaminants. Trans Am Fish Soc 115:596–609
Hamza-Chaffai A, Cosson RP, Amiard-Triquet C, El Abed A (1995) Physico-chemical forms of storage of metals (Cd, Cu and Zn) and metallothionein like protein in fish from the Tunisian coast, ecotoxicological consequences. Comp Biochem Physiol Part C 111:329–341
Hollis L, Hogstrand C, Wood CM (2001) Tissue-specific cadmium accumulation, metallothionein induction, tissue zinc and copper levels during chronic sublethal cadmium exposure in juvenile rainbow trout. Arch Environ Contam Toxicol 41:468–474
Jebali J, Banni M, Almeida EA, Bannaoui A, Boussetta H (2006) Effects of malathion and cadmium on acetylcholinesterase activity and metallothionein levels in the fish Seriola dumerilli. Fish Physiol Biochem 32:93–98
Jebali J, Banni M, De Almeida EA, Boussetta H (2007) Oxidative DNA damage levels and catalase activity in the clam Ruditapes decussatus as pollution biomarkers of Tunisian marine environment. Environ Monit Assess 124:195–200
Kàgi JHR, Kojima Y (1987) Chemistry and biochemistry of metallothionein. Experientia Suppl 52:25–61
Kraemer LD, Campbell PGC, Hare L (2005) Dynamics of Cd, Cu and Zn accumulation in organs and sub-cellular fractions in field transplanted juvenile yellow perch (Perca flavescens). Environ Pollut 138:324–337
Lanno RP, Hicks B, Hilton JW (1987) Histological observations on intrahepatocytic copper-containing granules in rainbow trout reared on diets containing elevated levels of copper. Aquat Toxicol 10:251–263
Marijić V, Raspor B (2006) Age and tissue-dependant metallothionein and cytosolic metal distribution in a native Mediterranean fish, Mullus barbatus, from the Eastern Adriatic sea. Comp Biochem Physiol Part C 143:382–387
Paris-Palacios S, Biagianti-Risbourg S, Fouley A, Vernet G (2000) Metallothionein in liver of Rutilus rutilus exposed to C2+. Analysis by metal summation, SH determination and spectrophuorimetry. Comp Biochem Physiol Part C 126:113–122
Rodriguez-Ariza A, Alhama J, Diaz-Mendez FM, Lopez-Barea J (1999) Content of oxodG in chromosomal DNA of Sparus aurata fish as biomarkers of oxidative stress and environmental pollution. Mutat Res 438:97–107
Roesijadi G (1992) Metallothioneins in metal regulation and toxicity in aquatic animals. Aquat Toxicol 22:81–114
Roesijadi G, Unger ME (1988) Immunochemical quantification of metallothioneins of a marine mollusk. Can J Fish Aquat Sci 45:1257–1263
Roesijadi G, Hansen KM, Unger ME (1997) Concentration-response relationship for Cd, Cu, and Zn and metallothionein mRNA induction in larvae of Crassostrea virginica. Comp Biochem Physiol 118C(3):267–270
Roméo M, Cosson RP, Gnassia-Barelli M, Risso C, Stien X, Lafaurie M (1997) Metallothionein determination in the liver of the sea bass Dicentrarchus labrax treated with copper and B(a)P. Mar Environ Res 44:275–284
Roméo M, Bennani M, Gnassia-Barelli M, Lafaurie M, Girard JP (2000) Cadmium and copper display different responses toxards oxidative stress in the kidney of the sea bass Dicentrarchus labrax. Aquatic Toxicol 48:185–194
Tom M, Moran O, Jabukov E, Cavari B, Rinkevitch B (1998) Molecular characterization of metallothionein-cDNA of Sparus aurata used for detecting heavy metal pollution along the Mediterranean coast of Israel. Mar Pollut Bull 36:131–137
Vaglio A, Landdriscina C (1999) Changes in liver enzyme activity in the teleost Sparus aurata in response to cadmium intoxication. Ecotoxicol Environ Saf 43:111–116
Van den Hurk P, Faisal M, Roberts MH Jr (2000) Interactive effects of cadmium and benzo-(a)-pyrene on metallothionein induction in mummichog (Fundulus heteroclitus). Mar Environ Res 50:83–87
Viarengo A (1989) Heavy metals in marine vertebrates: mechanisms of regulation and toxicity at the cellular level. Rev Aquat Sci 1:295–317
Viarengo A, Ponzano E, Dondero F, Fabbri R (1997) A simple spectrophotometric method for metallothionein evaluation in marine organism: an application to Mediterranean and Antarctic molluscs. Mar Environ Res 44:69–84
Viarengo A, Burlando B, Dondero F, Marro A, Fabbri R (1999) Metallothionein as a tool in biomonitoring programmes. Biomarkers 4:455–466
Wallace WG, Lee B-G, Luoma SN (2003) Sub-cellular compartmentalization of Cd and Zn in two bivalves. I. Significance of metal-sensitive fractions (MSF) and biologically detoxified metal (BDM). Mar Ecol Prog Ser 249:183–197
Weis P, Bogden JD, Enslee EC (1986) Hg- and Cu-induced hepatocellular changes in the mummichog, Fundulus heteroclitus. Environ Health Perspect 65:167–173
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This study was supported by a fund from the Ministry of Scientific Research and Technology, Tunisia (Unité de Recherche en Biochimie et Toxicologie Environnementale).
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Ghedira, J., Jebali, J., Bouraoui, Z. et al. Metallothionein and metal levels in liver, gills and kidney of Sparus aurata exposed to sublethal doses of cadmium and copper. Fish Physiol Biochem 36, 101–107 (2010). https://doi.org/10.1007/s10695-008-9295-1
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DOI: https://doi.org/10.1007/s10695-008-9295-1