Abstract
Pathogens face numerous challenges to persist in hosts with low or unpredictable population densities. Strategies include horizontal transmission, such as by the production of propagules that persist in the environment, and vertical transmission from adults to offspring. While many pathogens are capable of horizontal and vertical transmission little is known of their relative roles under realistic conditions of changing population densities. Insect baculoviruses can be transmitted both horizontally and vertically, although much of the work on baculovirus transmission has focussed on horizontal transmission that can be effective at high host densities. Here, we examine the prevalence of a vertically-transmitted, covert infection of nucleopolyhedrovirus (NPV) in field populations of the African armyworm, Spodoptera exempta, in Tanzania. African armyworm is a major pest of graminaceous crops in Africa and despite its migratory nature and boom and bust dynamics, NPV epizootics are common and can be intense at the end of the multi-generation armyworm season. We found that virtually all the insects collected in the field were positive for S. exempta NPV (SpexNPV) DNA and 60% of these insects had transcriptionally active virus. This suggests that SpexNPV is transmitted vertically at extremely high levels in field populations of S. exempta and can maintain a persistent infection without obvious symptoms. Similarly high levels of virus DNA and RNA were detected in a S. exempta colony that had been maintained in continuous culture for 5 years. This study provides an insight into mechanisms of pathogen persistence in migratory populations where hosts are unpredictable and indicates that covert infection may be more common and more relevant in disease dynamics of insects than had previously been thought.
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Acknowledgments
We would like to thank Tim Carty for rearing the laboratory colony of S. exempta and for producing insect diet and John Burden for advice on PCR. We are also very grateful to Wilfred Mushobozi and colleagues in Arusha, Tanzania, for their input and support in the field and David Gryzwacz for his encouragement and support throughout the project. We would also like to thank David Grzywacz and Judy Myers for their comments on the manuscript and Hilary Lauzon for help with Fig. 2. This work was funded by NERC grant NER/A/S/2000/01141 to JSC and KW and partly funded by the United Kingdom Department for International Development (DFID) for the benefit of developing countries. The views expressed are not necessarily those of the DFID R7954 Crop Protection Research Programme. JSC would also like to acknowledge the support of the NSERC Canada Research Chair program.
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Vilaplana, L., Wilson, K., Redman, E.M. et al. Pathogen persistence in migratory insects: high levels of vertically-transmitted virus infection in field populations of the African armyworm. Evol Ecol 24, 147–160 (2010). https://doi.org/10.1007/s10682-009-9296-2
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DOI: https://doi.org/10.1007/s10682-009-9296-2