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Identification and evaluation of resistance to Fusarium oxysporum f. sp. cubense tropical race 4 in Musa acuminata Pahang

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Abstract

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), seriously threatens global banana production. Identifying a reliable Foc TR4 resistance source and understanding the underlying defense mechanism is essential for future banana breeding programs. Pahang, a wild diploid Musa acuminata has displayed strong resistance to Foc TR4 in greenhouse tests. In the present study, Pahang and a highly susceptible cultivar, Brazilian, were inoculated with Foc TR4 tagged with green fluorescent protein (GFP-Foc TR4) and the infection processes were subsequently monitored. We found that, in a wounding inoculation system, by 7 days post-inoculation (dpi) the infection processes in roots of Pahang and Brazilian were similar, but that later, the colonization in Pahang corms was significantly delayed, compared with infection in Brazilian. Foc TR4 mycelia invaded root vascular bundles via wounds at 3 dpi, and reached the corm at 7 dpi in both accessions. However, the amount of fungal biomass and the degree of necrosis in Pahang corms were significantly less than their levels in Brazilian at 14 dpi. We further conducted a disease severity survey in both greenhouse and field trials and verified Foc TR4 resistance of Pahang. Our results indicate that suppression of further fungal proliferation in the corm is a possible mechanism of Fusarium wilt resistance in Pahang.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC31560505), Science and Technology Department of Yunnan Provincial Government (2015HA033 and 2015HA034), and National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences. We thank International Transit Centre for supplying the germplasm of M. acuminata Pahang (ITC0609), and we also thank Professor Huaping Li (South China Agricultural University, College of Natural Resources and Environment) for providing GFP-Foc TR4 strain. We are grateful Baoming Yang and Yuling Huang (Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences) for providing technical guidance of banana tissue in vitro culture technology. Vincent Johnson from Bioversity International is also gratefully acknowledged for his technical editing of this paper.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming, 650201, China

    Lei Zhang & Yunyue Wang

  2. Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650205, China

    Lei Zhang, Tingting Bai, Shengtao Xu & Si-Jun Zheng

  3. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China

    Tinglu Yuan, Yanzhang Wang, Dong Zhang & Weihua Tang

  4. Bioversity International, 2238 Beijing Road, Kunming, 650205, Yunnan, China

    Si-Jun Zheng

  5. Dehong Agricultural Technology Extension Center, Mangshi, 678400, China

    Lei Zhang

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  1. Lei Zhang
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Zhang, L., Yuan, T., Wang, Y. et al. Identification and evaluation of resistance to Fusarium oxysporum f. sp. cubense tropical race 4 in Musa acuminata Pahang. Euphytica 214, 106 (2018). https://doi.org/10.1007/s10681-018-2185-4

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