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QTL mapping and marker analysis of main stem height and the first lateral branch length in peanut (Arachis hypogaea L.)

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Abstract

Main stem height (MSH) and the first lateral branch length (LBL) of peanut (Arachis hypogaea L.) are important traits affecting plant shape and yield. Identification of quantitative trait loci (QTLs) related to these two traits, the prediction and cloning of candidate genes, and identification of plant height-related molecular markers are the basis for analysis of the molecular genetic mechanism of plant shape in peanut. In this study, a population of 151 recombinant inbred lines from a single seed, derived from a cross between variety 79266 (P1) and its variant progeny D893 (P2), was used to construct a peanut genetic map. The map consisted of 231 simple sequence repeat markers in 23 linkage groups, had a total length of 905.18 cM with average and minimum marker intervals of 3.92 and 0.1 cM, respectively. There were 11 and 16 QTLs detected in six environments for MSH and LBH with 6.26–22.53 and 5.89–21.63% phenotypic variation explained (PVE), respectively. Seven QTLs were detected in two or more environments: 3 QTLs for MSH (including Qmsh-14-3) with 7.66–22.53% PVE, and 4 QTLs for LBL (including Qllb-11-1) with 6.12–21.63% PVE. Qmsh-14-3 was steadily detected in five environments, localized between two markers, ARS376 and SEQ4G02, exhibited a genetic distance of 0.59–2.59 and 4.11–6.11 cM from the two markers, respectively. Qllb-11-1 was steadily detected in five environments, localized between two markers, ARS203 and AHS1413, exhibited a genetic distance of 1.06–3.06 and 0.23–2.23 cM from the two markers, respectively. There were 220 germplasm accessions used to detect the relationship between genotype and phenotypic values of traits at the marker loci ARS376 and SEQ4G02, average values of MSH and LBL were significantly higher for germplasm with the P1 compared to the P2 genotype. Determination of the marker loci ARS203 and AHS1413 indicated that average values of LBL were greater in germplasm with the P1 than the P2 genotype. The results provide references for fine mapping of QTLs for MSH and LBL, as well as breeding optimum plant-types in peanut.

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Abbreviations

QTL:

Quantitative trait locus/loci

RIL:

Recombinant inbred line

SSR:

Simple sequence repeat

MSH:

Main stem height

LBL:

The first lateral branch length

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Acknowledgements

This research was supported by the Natural Science Foundation of China (NSFC) (31571711), the Earmarked Fund for China Agriculture Research System (CARS-14), the Peanut Seed Industry Project in Shandong Province of China, the Earmarked Fund for Agriculture Research System in Shandong Province of China (SDAIT-04-03) and the Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province (Grant No. CX(12)5076).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, China

    Yingjie Li, Lanzhou Li, Xiurong Zhang, Kun Zhang, Jiaqi Liu, Fengzhen Liu & Yongshan Wan

  2. Jining Academy of Agricultural Sciences, Ji’ning, 272100, China

    Dengchao Ma

  3. Xuzhou Institute of Agricultural Sciences, Xuzhou, 221000, China

    Xiaojun Wang

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  1. Yingjie Li
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Correspondence to Fengzhen Liu or Yongshan Wan.

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Li, Y., Li, L., Zhang, X. et al. QTL mapping and marker analysis of main stem height and the first lateral branch length in peanut (Arachis hypogaea L.). Euphytica 213, 57 (2017). https://doi.org/10.1007/s10681-017-1847-y

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