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Cytogenetic characterization of the Passiflora edulis Sims × Passiflora cincinnata Mast. interspecific hybrid and its parents

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Abstract

The genus Passiflora L. consists of approximately 530 widely distributed species, including Passiflora edulis, which has drawn interest because of its commercial and agronomic value. Passiflora cincinnata is another important species owing to its long flowering period and resistance or tolerance to diseases and pests. In the present study, the meiotic segregation and pollen viability of an interspecific hybrid (P. edulis × P. cincinnata) and its parents were analyzed. The genomic contents were characterized using chromomycin A3 (CMA3)/4′-6′-diamidino-2-phenylindole (DAPI) staining, fluorescent in situ hybridization with 5S/45S ribosomal DNA (rDNA), genomic in situ hybridization (GISH), and inter-simple sequence repeat (ISSR) markers. The results indicated the diploid chromosome number for the parents and interspecific hybrid was 2n = 18. We also observed regular meiosis, one pair of 5S rDNA sites, and two pairs of 45S rDNA sites that co-localized with two pairs of CMA3 +/DAPI− bands. The GISH data revealed three distinct chromosomal groups in the hybrid. The genetic origins of the interspecific hybrid, and its relationship with its parents were also confirmed using ISSR markers.

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Acknowledgments

The authors thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for Grants to M. S. E. Coelho and N. F. Melo, and the Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) for its support.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Recursos Genéticos Vegetais, Universidade Estadual de Feira de Santana, Avenida Transnordestina S/N-Novo Horizonte, CEP 44036-900, Feira de Santana, BA, Brazil

    Maria do Socorro Evangelista Coelho

  2. Colegiado de Ciências Biológicas, Universidade Federal do Vale do São Francisco, Campus Ciências Agrárias, BR 407, km 12, Lote 543, CEP 56304-917, Petrolina, PE, Brazil

    Kyria Cilene de Andrade Bortoleti

  3. Embrapa Semiárido, Rodovia BR-428, km 152, Zona Rural, Caixa Postal 23, CEP 56302-970, Petrolina, PE, Brazil

    Francisco Pinheiro de Araújo & Natoniel Franklin de Melo

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  1. Maria do Socorro Evangelista Coelho
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  2. Kyria Cilene de Andrade Bortoleti
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  3. Francisco Pinheiro de Araújo
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  4. Natoniel Franklin de Melo
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Correspondence to Maria do Socorro Evangelista Coelho.

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Coelho, M.S.E., Bortoleti, K.C.A., Araújo, F.P. et al. Cytogenetic characterization of the Passiflora edulis Sims × Passiflora cincinnata Mast. interspecific hybrid and its parents. Euphytica 210, 93–104 (2016). https://doi.org/10.1007/s10681-016-1704-4

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