Skip to main content

Advertisement

SpringerLink
Log in

Inheritance of high iron and zinc concentration in selected bean varieties

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Micronutrient malnutrition especially iron (Fe) and zinc (Zn) has disastrous consequences for the more vulnerable members of the human society, especially poor women and pre-school children in developing countries. The common bean (Phaseolus vulgaris L.), an important grain legume directly consumed by humans provides cheap and important quantities of protein and calories and is an excellent source of some minerals (Fe and Zn) and vitamins which qualify it as perfect food. Unfortunately, many of the adapted bean market class varieties are low in these nutrients. Therefore there is need for biofortification of these varieties with Fe and Zn through plant breeding and genetic engineering in order to contribute to improved health of bean consumers. The objective of this study was to determine the mode of inheritance of high Fe and Zn concentration in selected bean varieties. Six parents high and low in Fe and Zn were crossed following a full diallel crossing design. Populations were advanced from F1 to F2. F3 seed were analyzed for Fe and Zn concentration using X-ray fluorescence. The study showed that both additive and non-additive gene effects are important in determining the expression of high Zn and Fe concentration even though additive gene effects contributed more. Narrow sense heritability of Fe and Zn was high estimated at 71 % for Fe and 83 % for Zn. Significant maternal and reciprocal effects suggest also that cytoplasmic inheritance is involved in Zn and Fe concentration. Strong positive correlation between Fe and Zn concentrations (r = 0.75) was observed suggesting that these micronutrients are not independently inherited. The positive relationship between Fe and Zn plus the negative relationship observed between Zn and seed size (r = 56) suggest that Zn and Fe accumulation is controlled multigenically or oligogenically with some genes affecting the concentration of both minerals. The variability in seed mineral concentration among crosses was larger for Fe (47–77 ppm) than for Zn (28–38 ppm).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Baker RJ (1978) Issues in diallel analysis. Crop Sci 18(4):533–536

    Article  Google Scholar 

  • Behera SK (2007) Estimation of heritability. http://www.iasri.res.in/seminar/AS-299/ebooks%5C2006-2007%5CMsc%5Ctrim2%5C3.%20Estimation%20of%20Heritability-Subrat%20Kumar%20Behera.pdf

  • Blair MW, Astudillo C, Grusak MA, Gram R, Beebe SE (2009) Inheritance of seed iron and zinc concentration in common bean (Phaseolus vulgaris L.). Mol Breed 23:197–207

    Article  CAS  Google Scholar 

  • Blair MW, Medina JI, Astudillo C, Judith R, Steve BE, Machado G, Gram R (2010) QTL for seed iron and zinc concentration and concentration in a Mesoamerican common bean (Phaseolus vulgaris L.) population. Theor Appl Gener 121:1059–1070

    Article  CAS  Google Scholar 

  • Bruce W (2001) Quantitative genetics. University of Arizona, Secondary article, Encyclopedia of life Sciences, 2001 Nature publishing group, Tucson

    Google Scholar 

  • Cichy K, Caldas G, Snapp S, Blair MW (2009) QTL analysis of seed iron, zinc, and phosphorus levels in an Andean bean population. Crop Sci 49:1742–1750

    Article  CAS  Google Scholar 

  • Coors JG (1999) Selection methodologies and heterosis. In: Coors SG, Pandey S (eds) The genetics and exploitation of heterosis in crops. ASA, CSS, and SSSA, Madison, pp 225–245

    Google Scholar 

  • Crusio WE (1987) A note on the analysis of reciprocal effects in diallel crosses. J Genet 66(3):177–185

    Article  Google Scholar 

  • Dabholkar AR (1992) Elements of biometrical genetics. Concept publishing company, New Delhi

    Google Scholar 

  • Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th edn. Longman, New York

    Google Scholar 

  • Fikret I (2009) Analysis of diallel mating design. North Calorina State University, Raleight USA. www4.ncsu.edu/~fisik/Analysis%20of%20Diallel%20Progeny%20Test%20with%20SAS.pdf. Accessed 10 July 2012, p 34

  • Gardner CO, Eberhart SA (1966) Analysis and interpretation of the variety cross diallel and related populations. Biometrics 22:439–452

    Article  CAS  PubMed  Google Scholar 

  • Griffing B (1956) Concept of general and specific combining ability in relation to diallel crossing systems. Aust J Biol Sci 9:463–493

    Google Scholar 

  • Gustavo AL, Potts BM, Vaillancourt RE, Apiolaza L (2003) Maternal and carryover effects on early growth of Eucalyptus globulus. Can J For Res 33:2108–2115

    Article  Google Scholar 

  • Hacisalihoglu G, Kochian LV (2003) How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants. New Phytol 159:341–350. doi:10.1046/j.1469-8137.2003.00826.x

    Article  CAS  Google Scholar 

  • Harvest Plus (2011) Breeding crops for better nutrition. http://www.harvestplus.org/content/learn-more

  • Hayman BI (1954) The analysis of variance of diallel tables. Biometrics 10:235–244

    Article  Google Scholar 

  • Hill WG, Goddard ME, Visscher PM (2008) Data and theory point to mainly additive genetic variance for complex traits. PLoS Genet 4(2):e1000008. doi:10.1371/journal.pgen.1000008

    Article  PubMed Central  PubMed  Google Scholar 

  • House WA, Welch RM, Beebe SE, Cheng Z (2002) Potential for increasing the amounts of bioavailable zinc in dry beans (Phaseolus vulgaris L) through plant breeding. J Sci Food Agric 82:1452–1457

    Article  CAS  Google Scholar 

  • Islam FMA, Basford KE, Jara C, Redden RJ, Beebe SE (2002) Seed compositional and disease resistance differences among gene pools in cultivated common bean. Genet Resour Crop Evol 49:285–293

    Article  Google Scholar 

  • Jinks JL (1955) A survey of a genetical basis of heterosis in a variety of diallel crosses. Heredity 9:223–238

    Article  Google Scholar 

  • Jinks JL (1956) The F2 and backcross generation from a set of diallel crosses. Heredity 10:1–30

    Article  Google Scholar 

  • Johnson H, Robinson H, Comstock RE (1955) Estimation of genetic and environmental variability in soybeans. Agron J 47:314–331

    Article  Google Scholar 

  • Jones RM (1965) Analysis of variance of the half diallel table. Heredity 20(1):117–121

    Article  Google Scholar 

  • Khatun MT, Bari MAA, Zaman MA, Begum H, Akter S (2010) Heterosis estimates in F2 diallel population of spring wheat at two different cultural conditions. Bangladesh J Agric Res 35(3):413–422

    Google Scholar 

  • Nchimbi-Msolla S, Muhamba GTM (2010) The effects of the environment on iron and zinc concentrations and performance of common bean (Phaseolus vulgaris L.) genotypes. Asian J Plant Sci 9:455–462

    Article  CAS  Google Scholar 

  • Noubissié JBT, Bell JM, Yadji HT (2012) Studies on variability and gene effects of seed protein concentrations in Cameroonian bean (Phaseolus vulgaris L.) cultivars. J Agric Soc Sci 8:17–23

    Google Scholar 

  • Paltridge et al (2012) Energy-dispersive X-ray fluorescence analysis of zinc and iron concentration in rice and pearl millet grain. Plant Soil. doi:10.1007/s11104-011-1104-4

    Google Scholar 

  • Panni MK, Khan NU, Fitmawati SB, Bibi M (2012) Heterotic studies and inbreeding depression in F2 populations of upland cotton. Pak J Bot 44(3):1013–1020

    Google Scholar 

  • Peleg Z, Yehoshua S, Atilla Y, Tzion F, Levent O, Ismail C (2008) Grain zinc, iron and protein concentrations and zinc-efficiency in wild emmer wheat under contrasting irrigation regimes. Plant Soil 306:57–67

    Article  CAS  Google Scholar 

  • Pfeiffer WH, McClafferty B (2007) Biofortification: breeding micronutrient-dense-crop. In: Kang MS, Priyadarshan PM (eds) Breeding major food staples for the 21st century, chap 3. Blackwell Scientific: Oxford, pp 61–91

  • Rinke EH, Hayes HK (1963) General and specific combining ability in diallel crosses of 15 inbred lines of corn. Bot Bull Acad Sin 5:36–41

    Google Scholar 

  • Roach DA, Wulff RD (1987) Maternal effects in plants. Annu Rev Ecol Syst 18:209–235

    Article  Google Scholar 

  • Tadesse T, Tesso T, Ejeta G (2008) Combining ability of introduced sorghum parental lines for major morpho-agronomic traits. J SAT Agric Res 6:1–7

    Google Scholar 

  • Variath MT, Wu JG, Li YX, Chen GL, Shi CH (2009) Genetic analysis for oil and protein concentrations in rapeseed (Brassica napus) at different developmental times. Euphytica 166:145–153

    Article  CAS  Google Scholar 

  • VSN International Ltd (2012) http://genstat.software.informer.com/download/

  • Zhang J, Wang M, Cao Y, Wu L, Xu S (2012) Iron and zinc accumulation trend in a Japonica rice grains after anthesis. Afr J Agric Res 7(8):1312–1316

    Google Scholar 

Download references

Acknowledgments

We are very grateful to Makerere University, AGRA, CIAT, Harvest plus project and RAB for the support to this work.

Author information

Authors and Affiliations

  1. Department of Agricultural Production, Makerere University, P. O. Box 7062, Kampala, Uganda

    F. Mukamuhirwa & G. Tusiime

  2. CIAT Africa, National Agricultural Research Laboratories- Kawanda, P. O. Box 7065, Kampala, Uganda

    M. C. Mukankusi

Authors
  1. F. Mukamuhirwa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Tusiime
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. C. Mukankusi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to F. Mukamuhirwa.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mukamuhirwa, F., Tusiime, G. & Mukankusi, M.C. Inheritance of high iron and zinc concentration in selected bean varieties. Euphytica 205, 349–360 (2015). https://doi.org/10.1007/s10681-015-1385-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-015-1385-4

Keywords

Search

Navigation