Abstract
Cotton is a leading natural fiber crop in the textile industry worldwide. The improvement of cotton fiber quality has become more important because of changes in spinning technology and ever-increasing demands. Mapping quantitative trait locus (QTL) for fiber quality traits will enable molecular marker-assisted selection (MAS) to improve fiber quality and provide an access to reveal the molecular mechanism of fiber development. A high-density intraspecific genetic map is constructed based on an upland cotton recombinant inbred line (RIL) population. A total of 25,313 SSR primer pairs were used and yielded 1,333 polymorphic markers, with a polymorphic ratio of 5.3 %, producing 1,382 polymorphic loci in the RIL population. The map comprised 1,274 loci and spanned 3,076.4 cM with an average distance of 2.41 cM between two adjacent markers. Based on the phenotypic data of fiber quality traits from five environments, a total of 59 QTL were detected. These QTL comprised 15 QTL for fiber upper half mean length, 10 QTL for fiber length uniformity, 9 QTL for fiber strength, 10 QTL for fiber elongation and 15 QTL for fiber micronaire, respectively. The genetic map constructed in this study is the most detailed upland cotton intraspecific map based on SSR markers to date, and could be used to construct consensus map or as reference genetic map for tetraploid cotton genome assembly. Stable QTL identified across multiple environments reflect some important and favorable alleles shaping fiber quality, and they are valuable candidate alleles for MAS breeding projects as well as for gene function research related to cotton fiber development and quality improvement.
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References
Al-Ghazi Y, Bourot S, Arioli T, Dennis ES, Llewellyn DJ (2009) Transcript profiling during fiber development identifies pathways in secondary metabolism and cell wall structure that may contribute to cotton fiber quality. Plant Cell Physiol 50:1364–1381
Blenda A, Fang DD, Rami JF, Garsmeur O, Luo F, Lacape JM (2012) A high density consensus genetic map of tetraploid cotton that integrates multiple component maps through molecular marker redundancy check. PLoS One 7:e45739
Chen L, Zhang ZS, Hu MC, Wang W, Zhang J, Liu DJ, Zheng J, Zheng FM, Ma J (2008) Genetic linkage map construction and QTL mapping for yield and fiber quality in upland cotton. Acta Agron Sin 34:1199–1205 (in Chinese with English abstract)
Culp TW, Lewis CF (1973) Breeding methods for improving yield and fiber quality of upland cotton (Gossypium hirsutum). Crop Sci 13:686–689
Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ (2007) A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium. Genetics 176:527–541
Guo WZ, Cai CP, Wang CB, Zhao L, Wang L, Zhang TZ (2008) A preliminary analysis of genome structure and composition in Gossypium hirsutum. BMC Genomics 9:314
Hovav R, Udall JA, Hovav E, Rapp R, Flagel LE, Wendel JF (2008) A majority of cotton genes are expressed in single-celled fiber. Planta 227:319–329
Hu WJ, Zhang XY, Zhang TZ, Guo WZ (2008) Molecular tagging and source analysis of QTL for elite fiber quality in upland cotton. Acta Agron Sin 34:578–586 (in Chinese with English abstract)
Iqbal J, Reddy OUK, El-Zik KM, Pepper AE (2001) A genetic bottleneck in the ‘evolution under domestication’ of Upland cotton Gossypium hirsutum L. examined using DNA fingerprinting. Theor Appl Genet 103:547–554
Lacape JM, Nguyen TB, Thibivilliers S, Bojinov B, Courtois B, Cantrell RG, Burr B, Hau B (2003) A combined RFLP–SSR–AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population. Genome 46:612–626
Lacape JM, Dessauw D, Rajab M, Noyer JL, Hau B (2007) Microsatellite diversity in tetraploid Gossypium germplasm: assembling a highly informative genotyping set of cotton SSRs. Mol Breed 19:45–58
Lacape JM, Llewellyn D, Jacobs J, Arioli T, Becker D, Calhoun S, Al-Ghazi Y, Liu SM, Palaï O, Georges S, Giband M, de Assunção H, Barroso V, Claverie M, Gawryziak G, Jean J, Vialle M, Viot C (2010) Meta-analysis of cotton fiber quality QTLs across diverse environments in a Gossypium hirsutum × G. barbadense RIL population. BMC Plant Biol 10:132
Lee JJ, Woodward AW, Chen ZJ (2007) Gene expression changes and early events in cotton fibre development. Ann Bot 100:1391–1401
Lin ZX, Zhang YX, Zhang XL, Guo XP (2009) A high-density integrative linkage map for Gossypium hirsutum. Euphytica 166:35–45
Ni HJ, Wang W, Teng ZH, Zhang J, Liu DH, Zhang ZS (2011) QTL mapping of yield and fiber quality traits in upland cotton. J Southwest Univ (Nat Sci Ed) 33:7–14 (in Chinese with English abstract)
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksely SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Paterson AH, Saranga Y, Menz M, Jiang CX, Wright RJ (2003) QTL analysis of genotype by environment interaction affecting cotton fiber quality. Theor Appl Genet 106:384–396
Paterson AH, Wendel JF, Gundlach H, Guo H, Jenkins J, Jin D, Llewellyn D, Showmaker KC, Shu S, Udall J, Yoo MJ, Byers R, Chen W, Doron-Faigenboim A, Duke MV, Gong L, Grimwood J, Grover C, Grupp K, Hu G, Lee TH, Li J, Lin L, Liu T, Marler BS, Page JT, Roberts AW, Romanel E, Sanders WS, Szadkowski E, Tan X, Tang H, Xu C, Wang J, Wang Z, Zhang D, Zhang L, Ashrafi H, Bedon F, Bowers JE, Brubaker CL, Chee PW, Das S, Gingle AR, Haigler CH, Harker D, Hoffmann LV, Hovav R, Jones DC, Lemke C, Mansoor S, ur Rahman M, Rainville LN, Rambani A, Reddy UK, Rong JK, Saranga Y, Scheffler BE, Scheffler JA, Stelly DM, Triplett BA, Van Deynze A, Vaslin MF, Waghmare VN, Walford SA, Wright RJ, Zaki EA, Zhang T, Dennis ES, Mayer KF, Peterson DG, Rokhsar DS, Wang X, Schmutz J (2012) Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature 492:423–427
Percival AE, Wendel JF, Stemart JM (1999) Taxonomy and germplasm resourses. In: Smith CW, Cothren JT (eds) Cotton: origin, history, technology, and production. Wiley, New York, pp 33–63
Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH (1994) A detailed RFLP map of cotton Gossypium hirsutum × Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. Genetics 138:829–847
Rong JK, Abbey C, Bowers JE, Brubaker CL, Chang C, Chee PW, Delmonte TA, Ding X, Garza JJ, Marler BS, Park C, Pierce GJ, Rainey KM, Rastogi VK, Schulze SR, Tronlinde NL, Wendel JF, Wilkins TA, Wing RA, Wright RJ, Zhao X, Zhu L, Paterson AH (2004) A 3347-locus genetic recombination map of sequence-tagged sites reveals features of genome organization, transmission and evolution of cotton (Gossypium). Genetics 166:389–417
Rong JK, Feltus FA, Waghmare VN, Pierce GJ, Chee P, Draye X, Saranga Y, Wright RJ, Wilkins TA, May LO, Smith CW, Gannaway JR, Wendel JF, Paterson AH (2007) Meta-analysis of polyploid cotton QTL shows unequal contributions of subgenomes to a complex network of genes and gene clusters implicated in lint fiber development. Genetics 176:2577–2588
Rungis D, Llewellyn D, Dennis ES, Lyon BR (2005) Simple sequence repeat (SSR) markers reveal low levels of polymorphism between cotton (Gossypium hirsutum L.) cultivars. J Agric Res 56:301–307
Said JI, Lin ZX, Zhang XL, Song MZ, Zhang JF (2013) A comprehensive meta QTL analysis for fiber quality, yield, yield related and morphological traits, drought tolerance, and disease resistance in tetraploid cotton. BMC Genomics 14:776
Shen XL, Guo WZ, Zhu XF, Yuan YL, Yu JZ, Kohel RJ, Zhang TZ (2005) Molecular mapping of QTLs for fiber qualities in three diverse lines in upland cotton using SSR markers. Mol Breed 15:169–181
Shen XL, Guo WZ, Lu QX, Zhu XF, Yuan YL, Zhang TZ (2007) Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in upland cotton. Euphytica 155:371–380
Smith CW, Coyle GG (1997) Association of fiber quality parameters and within-boll yield components in upland cotton. Crop Sci 37:1775–1779
Sun FD, Zhang JH, Wang SF, Gong WK, Shi YZ, Liu AY, Li JW, Gong JW, Shang HH, Yuan YL (2012) QTL mapping for fiber quality traits across multiple generations and environments in upland cotton. Mol Breed 188:441–451
Taliercio EW, Boykin D (2007) Analysis of gene expression in cotton fiber initials. BMC Plant Biol 7:22
Tang SY, Teng ZH, Zhai TF, Fang XM, Liu F, Liu DJ, Zhang J, Liu DX, Wang SF, Zhang K, Shao QS, Tan ZY, Paterson AH, Zhang ZS (2014) Construction of genetic map and QTL analysis of fiber quality traits for Upland cotton (Gossypium hirsutum L.). Euphytica (Online). doi:10.1007/s10681-014-1189-y
Tanksley SD, Hewitt J (1988) Use of molecular markers in breeding for soluble solids content in tomato—a re-examination. Theor Appl Genet 75:811–823
Taylor DR, Ingvarsson PK (2003) Common features of segregation distortion in plants and animals. Genetica 117:27–35
Van Ooijen JW (2009) MapQTL 6.0. Software for the mapping of quantitative trait loci in experimental populations. Plant Research International, Wageningen
Van Ooijen JW, Voorrips RE (2006) JoinMap 4.0, software for the calculation of genetic linkage maps. Plant Research International, Wageningen
Voorrips RE (2006) MapChart 2.2: Software for the graphical presentation of linkage maps and QTLs. Plant Research International, Wageningen
Wang FR, Xu ZZ, Sun R, Gong YC, Liu GD, Zhang JX, Wang LM, Zhang CY, Fan SJ, Zhang J (2013) Genetic dissection of the introgressive genomic components from Gossypium barbadense L. that contribute to improved fiber quality in Gossypium hirsutum L. Mol Breed 32:547–562
Xiao J, Wu K, Fang DD, Stelly DM, Yu J, Cantrell RG (2009) New SSR markers for use in cotton (Gossypium spp.) improvement. J Cotton Sci 13:75–157
Xu YB, Zhu LH, Huang N, McCouch SR (1997) Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.). Mol Gen Genomics 253:535–545
Yu Y, Yuan DJ, Liang SG, Li XM, Wang XQ, Lin ZX, Zhang XL (2011) Genome structure of cotton revealed by a genome-wide SSR genetic map constructed from a BC1 population between Gossypium hirsutum and G. barbadense. BMC Genomics 12:15
Yu JW, Zhang K, Li SY, Yu SX, Zhai HH, Wu M, Li XL, Fan SL, Song MZ, Yang DG, Li YH, Zhang JF (2013) Mapping quantitative trait loci for lint yield and fiber quality across environments in a Gossypium hirsutum × Gossypium barbadense backcross inbred line population. Theor Appl Genet 126:275–287
Zhang ZS, Xiao YH, Luo M, Li XB, Luo XY, Hou L, Li DM, Pei Y (2005) Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.). Euphytica 144:91–99
Zhang YX, Lin ZX, Xia QZ, Zhang MJ, Zhang XL (2008) Characteristics and analysis of simple sequence repeats in the cotton genome based on a linkage map constructed from a BC1 population between Gossypium hirsutum and G. barbadense. Genome 51:534–546
Zhang ZS, Hu MC, Zhang J, Liu DJ, Zheng J, Zhang K, Wang W, Wan Q (2009) Construction of a comprehensive PCR-based marker linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.). Mol Breed 24:49–61
Zhang K, Zhang J, Ma J, Tang SY, Liu DJ, Teng ZH, Liu DX, Zhang ZS (2012) Genetic mapping and quantitative trait locus analysis of fiber quality traits using a three-parent composite population in upland cotton (Gossypium hirsutum L.). Mol Breed 29:335–348
Zhao L, Lv YD, Cai CP, Tong XC, Chen XD, Zhang W, Du H, Guo XH, Guo WZ (2012) Toward allotetraploid cotton genome assembly: integration of a high-density molecular genetic linkage map with DNA sequence information. BMC Genomics 13:539
Acknowledgments
This study was financially supported by the Natural Science Foundation of China (31071464, 31271770), Hi-tech Research and Development Program of China (2012AA101108) and the 111 Project (B12006).
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Zhaoyun Tan and Xiaomei Fang contributed equal work to this paper.
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Tan, Z., Fang, X., Tang, S. et al. Genetic map and QTL controlling fiber quality traits in upland cotton (Gossypium hirsutum L.). Euphytica 203, 615–628 (2015). https://doi.org/10.1007/s10681-014-1288-9
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DOI: https://doi.org/10.1007/s10681-014-1288-9