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Improving the resistance of eggplant (Solanum melongena) to Verticillium wilt using wild species Solanum linnaeanum

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An Erratum to this article was published on 28 September 2015

Abstract

Verticillium wilt, caused by Verticillium dahliae, is a soil-borne disease of the cultivated eggplant (Solanum melongena). The accession PI388846 from a wild species S. linnaeanum, shows resistance to Verticillium wilt. The introgression of its disease resistance gene into cultivated eggplants would allow for breeding disease resistant eggplants. In this study, interspecific hybridization and subsequent backcrossing between PI388846 and cultivated eggplants were performed. The results showed that Verticillium wilt resistance was successfully introduced into the cultivated eggplants, and the agronomic traits of the interspecific hybrid progeny were improved by continuous backcrossing with the cultivated eggplants. In addition, a gene specific marker for the Ve homolog in PI388846 was developed to detect Verticillium wilt resistance in the backcross population. The results represent a positive beginning for the genetic enhancement of cultivated eggplants for Verticillium wilt resistance.

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References

  • Acciarri N, Rotino GL, Valentino D, Vitelli G, Sunseri F, Martelli G, Tamietti G (2001) Improvement of eggplant for resistance to Verticillium through interspecific hybridization with Solanum sodomeum L. In: Proceedings of the 11th Eucarpia meeting, pp 327–338

  • Acciarri N; Sabatini E; Voltattorni S, Ciriaci T, Tamietti G, Valentino D, Mennella G, Cavallanti F, Tacconi MG, Toppino L, Grazioli G, Pedretti R, Alberti P, Rotino GL (2007) First eggplant pure lines of different typologies derived from sexual and somatic hybridization with S. sodomaeum and S. aethiopicum gr. aculeatum and gr. gilo. In: Proceedings of the 13th Eucarpia meeting, pp 327–338

  • Bae J, Halterman D, Jansk S (2008) Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific potato hybrids. Mol Breed 22:61–69

    Article  CAS  Google Scholar 

  • Bundock PC, Cross MJ, Shapter FM, Henry RJ (2006) Robust allele-specific polymerase chain reaction markers developed for single nucleotide polymorphisms in expressed barley sequences. Theor Appl Genet 112:358–365

    Article  CAS  PubMed  Google Scholar 

  • Collonnier C, Fock I, Kashyap V, Rotino GL, Daunay MC, Lian Y, Mariska IK, Rajam MV, Servaes A, Ducreux G, Sihachakr D (2001) Applications of biotechnology in eggplant. Plant Cell Tissue Org 65:91–107

    Article  CAS  Google Scholar 

  • Daniell H, Lee S, Grevich J, Saski C, Quesada-Vargas T, Guda C, Tomkins J, Jansen RK (2006) Complete chloroplast genome sequences of Solanum bulbocastanum, Solanum lycopersicum and comparative analyses with other Solanaceae genomes. Theor Appl Genet 112:1503–1518

    Article  CAS  PubMed  Google Scholar 

  • Daunay MC (2008) Eggplant. In: Prohens J, Nuez F (eds) Handbook of crop breeding., Vegetables: Fabaceae, Liliaceae, Umbelliferae, and SolanaceaeSpringer, New York, pp 163–220

    Google Scholar 

  • Diwan N, Fluhr R, Eshed Y, Zamir D, Tanksley SD (1999) Mapping of Ve in tomato: a gene conferring resistance to the broad-spectrum pathogen Verticillium dahliae race 1. Theor Appl Genet 98:315–319

    Article  CAS  Google Scholar 

  • Doganlar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002a) A comparative genetic linkage map of eggplant (Solanum melongena) and its implications for genome evolution in the Solanaceae. Genetics 161:1697–1711

    PubMed Central  CAS  PubMed  Google Scholar 

  • Doganlar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002b) Conservation of gene function in the solanaceae as revealed by comparative mapping of domestication traits in eggplant. Genetics 161:1713–1726

    PubMed Central  CAS  PubMed  Google Scholar 

  • Fradin EF, Thomma BPHJ (2006) Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Mol Plant Pathol 7:71–86

    Article  CAS  PubMed  Google Scholar 

  • Fradin EF, Zhang Z, Juarez Ayala JC, Castroverde CDM, Nazar RN, Robb J, Liu CM, Thomma BPHJ (2009) Genetic dissection of verticillium wilt resistance mediated by tomato Ve1. Plant Physiol 150:320–332

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Frary A, Doganlar S, Daunay MC, Tanksley SD (2003) QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species. Theor Appl Genet 107:359–370

    Article  CAS  PubMed  Google Scholar 

  • Hayes RJ, McHale LK, Vallad GE, Truco MJ, Michelmore RW, Klosterman SJ, Maruthachalam K, Subbarao KV (2011) The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theor Appl Genet 123:509–517

    Article  CAS  PubMed  Google Scholar 

  • Kawchuk LM, Hachey J, Lynch DR, Kulcsar F, van Rooijen G, Waterer DR, Robertson A, Kokko E, Byers R, Howard RJ, Fischer R, Prüfer D (2001) Tomato Ve disease resistance genes encode cell surface-like receptors. Proc Natl Acad Sci USA 98:6511–6515

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • King SR, Davis AR, Zhang XP, Crosby K (2010) Genetics, breeding and selection of rootstocks for Solanaceae and Cucurbitaceae. Sci Hortic-Amsterdam 127:106–111

    Article  Google Scholar 

  • Lee JM (1994) Cultivation of grafted vegetables I. Current status, grafting methods, and benefits. Hortscience 29:235–239

    Google Scholar 

  • Liu J, Huang S, Sun M, Liu S, Liu Y, Wang W, Zhang X, Wang H, Hua W (2012) An improved allele-specific PCR primer design method for SNP marker analysis and its application. Plant Methods 8:34

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Mace ES, Lester RN, Gebhardt CG (1999) AFLP analysis of genetic relationships among the cultivated eggplant, Solanum melongena L., and wild relatives (Solanaceae). Theor Appl Genet 99:626–633

    Article  CAS  PubMed  Google Scholar 

  • Mueller LA, Solow TH, Taylor N, Skwarecki B, Buels R, Binns J, Lin C, Wright MH, Ahrens R, Wang Y, Herbst EV, Keyder ER, Menda N, Zamir D, Tanksley SD (2005) The SOL genomics network: a comparative resource for solanaceae biology and beyond. Plant Physiol 138:1310–1317

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Murray HG, Thompson WF (1980) Rapid isolation of higher weight DNA. Nucleic Acids Res 8:4321

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Sunseri F, Sciancalepore A, Martelli G, Acciarri N, Rotino GL, Valentino D, Tamietti G (2003) Development of RAPD-AFLP map of eggplant and improvement of tolerance to verticillium wilt. Acta Hort (ISHS) 625:107–115

    CAS  Google Scholar 

  • Tjamos EC, Beckman CH (1989) Vascular wilt diseases of plants: Basic studies and control., NATO ASI Series H: Cell BiologySpringer-Verlag, Berlin

    Book  Google Scholar 

  • Uribe P, Jansky S, Halterman D (2014) Two CAPS markers predict Verticillium wilt resistance in wild Solanum species. Mol Breed 33:465–476

    Article  CAS  Google Scholar 

  • Vining K, Davis T (2009) Isolation of a Ve homolog, mVe1, and its relationship to verticillium wilt resistance in Mentha longifolia (L.) Huds. Mol Genet Genomics 282:173–184

    Article  CAS  PubMed  Google Scholar 

  • Wang Z, Xie C, Jue DW, Huang LP, Huang QS, Yang Q (2010) Cloning and expression analysis of Verticillium wilt Pathogenesis related gene StDAHP in Solanum torvum. China Biotechnol 30(6):48–53

    Google Scholar 

  • Wu F, Eannetta NT, Xu Y, Tanksley SD (2009a) A detailed synteny map of the eggplant genome based on conserved ortholog set II (COSII) markers. Theor Appl Genet 118:927–935

    Article  CAS  PubMed  Google Scholar 

  • Wu F, Eannetta NT, Xu Y, Durrett R, Mazourek M, Jahn MM, Tanksley SD (2009b) A COSII genetic map of the pepper genome provides a detailed picture of synteny with tomato and new insights into recent chromosome evolution in the genus Capsicum. Theor Appl Genet 118:1279–1293

    Article  CAS  PubMed  Google Scholar 

  • Yang L, Xie C, Li W, Zhang RJ, Jue DW, Yang Q (2013) Expression of a wild eggplant ribosomal protein L13a in potato enhances resistance to Verticillium dahliae. Plant Cell Tissue Organ Cult 115:329–340

    Article  CAS  Google Scholar 

  • Zhang W, Gianibelli MC, Ma W, Rampling L, Gale KR (2003) Identification of SNPs and development of allele-specific PCR markers for gamma-gliadin alleles in Triticum aestivum. Theor Appl Genet 107:130–138

    Article  CAS  PubMed  Google Scholar 

  • Zhang B, Yang Y, Chen T, Yu W, Liu T, Li H, Fan X, Ren Y, Shen D, Liu L, Dou D, Chang Y (2012) Island cotton Gbve1 gene encoding a receptor-like protein confers resistance to both defoliating and non-defoliating isolates of Verticillium dahliae. PLoS ONE 7(12):e51091

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Acknowledgments

This research is partially supported by the National High Technology Research and Development Program of China (2012AA100103), Natural Science Foundation (BK20131335) and Independent Innovation Foundation of Agricultural Sciences [CX (12)5046] of Jiangsu Province, China.

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Authors and Affiliations

  1. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, 50 Zhongling Street, Nanjing, 210014, Jiangsu, China

    Jun Liu, Zisong Zheng, Xiaohui Zhou, Cui Feng & Yong Zhuang

Authors
  1. Jun Liu
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  2. Zisong Zheng
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  3. Xiaohui Zhou
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  4. Cui Feng
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  5. Yong Zhuang
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Corresponding author

Correspondence to Yong Zhuang.

Additional information

Jun Liu and Zisong Zheng contributed equally to this study.

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Liu, J., Zheng, Z., Zhou, X. et al. Improving the resistance of eggplant (Solanum melongena) to Verticillium wilt using wild species Solanum linnaeanum . Euphytica 201, 463–469 (2015). https://doi.org/10.1007/s10681-014-1234-x

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  • DOI: https://doi.org/10.1007/s10681-014-1234-x

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