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Mapping the QTLs underlying drought stress at developmental stage of sorghum (Sorghum bicolor (L.) Moench) by association analysis

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Abstract

This study was conducted to identify quantitative trait loci (QTLs) for drought tolerance in sorghum (Sorghum bicolor (L.) Moench) by association mapping using a simple sequence repeat (SSR)-marker-based diversity research set. Genotypic data for 98 SSR marker loci on ten chromosomes were used for the association analysis. The experiment was conducted under control (well-watered) and drought stress conditions, and the phenotypic values of 23 morphological traits were recorded. Drought tolerance was assessed by using a leaf drying score as a parameter of the tolerance/susceptibility: scores were assigned on a scale from 1 (most tolerant) to 9 (most susceptible). Under the control conditions, 17 QTLs associated with 12 traits were identified on chromosomes 1, 2, 4, 8, 9, and 10, with −Log10 (P) ranging from 2.5 to 7.6 and explaining 9.5–57.5 % of the total phenotypic variance for the traits. Under the drought stress conditions, nine QTLs associated with 8 traits were identified on chromosomes 1, 2, 3, and 10 that explained 9–61.2 % of the total phenotypic variance for the traits, with −Log10 (P) ranging from 2.5 to 3.5. QTLs for some traits were detected only under the drought stress condition, suggesting that these traits are important in drought tolerance. These QTLs could be used to further dissect the genetic and physiological basis of drought tolerance in sorghum.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan

    Shazia Sakhi, Tariq Shehzad & Kazutoshi Okuno

  2. Department of Plant Science, Kohat University of Science & Technology, Kohat, 26000, Pakistan

    Shazia Sakhi & Shafiqur Rehman

  3. Alliance for Research on North Africa (ARENA), University of Tsukuba, Tennodai 1-1-1, Tsukuba, 305-8572, Japan

    Tariq Shehzad & Kazutoshi Okuno

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  1. Shazia Sakhi
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  2. Tariq Shehzad
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  3. Shafiqur Rehman
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  4. Kazutoshi Okuno
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Correspondence to Kazutoshi Okuno.

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Shazia Sakhi and Tariq Shehzad contributed equally to this study.

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Sakhi, S., Shehzad, T., Rehman, S. et al. Mapping the QTLs underlying drought stress at developmental stage of sorghum (Sorghum bicolor (L.) Moench) by association analysis. Euphytica 193, 433–450 (2013). https://doi.org/10.1007/s10681-013-0963-6

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