Abstract
Fine mapping of a quantitative trait locus, qFLL6.2, controlling flag leaf length (FLL) and yield traits in rice was conducted using four sets of near isogenic lines (NILs) that were developed from a common residual heterozygote at F7 generation of the indica rice cross Zhenshan 97/Milyang 46. Each of the NIL sets consisted of 40 lines that are S1 progenies of ten maternal homozygotes, ten paternal homozygotes, and 20 heterozygotes differing in a portion of the 1.19-Mb interval RM3414–RM6917 on the short arm of rice chromosome 6. Analysis of phenotypic differences among the three genotypic groups in each NIL set delimited qFLL6.2 to a 62.1-kb region flanked by simple sequence repeat marker RM3414 and sequence-tagged site marker Si2944. This QTL explained 52.73% of the phenotypic variance, and the Zhenshan 97 allele increased FLL by 2.40 cm. Based on data collected from homozygous lines of three of the NIL sets, qFLL6.2 was shown to have major effects on all the three yield traits analyzed, including the number of spikelets per panicle, the number of filled grains per panicle, and grain weight per panicle. A comparison of the different groups revealed that the effect of qFLL6.2 was highly consistent across different genetic backgrounds and environments, providing a good candidate for map-based cloning and investigating the source–sink relationship in rice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cao G-Q, Gao Y-M, Zhu J (2007) QTL analysis for flag leaf length in a rice DH population under multiple environments (in English with Chinese abstract). Acta Agron Sinica 33:223–229
Chen X, Temnykh S, Xu Y, Cho YG, McCouch SR (1997) Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor Appl Genet 95:553–567
Cui KH, Peng SB, Xing YZ, Yu SB, Xu CG, Zhang Q (2003) Molecular dissection of the genetic relationships of source, sink and transport tissue with yield traits in rice. Theor Appl Genet 106:649–658
Dai W-M, Zhang K-Q, Wu J-R, Wang L, Duan B-W, Zheng K-L, Cai R, Zhuang J-Y (2008) Validating a segment on the short arm of chromosome 6 responsible for genetic variation in the hull silicon content and yield traits of rice. Euphytica 160:317–324
Du J-H, Fan Y-Y, Wu J-R, Zhuang J-Y (2008) Dissection of QTLs for yield traits on the short arm of rice chromosome 6. Agric Sci China 7:513–520
Fageria NK (2007) Yield physiology of rice. J Plant Nutr 30:843–879
Fan G-Z, Dong Y-J, Wang C-M, Wan J-M, Xie H, Xu C-L, Zhu J-G, Cai Q-S (2007) Analysis of QTLs for flag leaf shape and its response to elevated CO2 in rice (Oryza sativa). Rice Sci 14:7–12
Ghosh S, Sahai VN, Saran S (1990) Role of flag leaf on grain yield and spikelet sterility in rice cultivar. Oryza 27:87–89
Jebbouj B, Yousfi BE (2009) Barley yield losses due to defoliation of upper three leaves either healthy or infected at boot stage by Pyrenophora teres f. teres. Eur J Plant Pathol 125:303–315
Jiang S, Zhang X, Wang J, Chen W, Xu Z (2010) Fine mapping of the quantitative trait locus qFLL9 controlling flag leaf length in rice. Euphytica 176:341–347
Kobayashi S, Fukuta Y, Morita S, Sato T, Osaki M, Khush GS (2003) Quantitative trait loci affecting flag leaf development in rice (Oryza sativa L.). Breed Sci 53:255–262
Kobayashi S, Araki E, Osaki M, Khush GS, Fukuta Y (2006) Localization, validation and characterization of plant-type QTLs on chromosomes 4 and 6 in rice (Oryza sativa L.). Field Crops Res 96:106–112
Li G, Xue L, Gu W, Yang G, Wang S, Ling Q, Qin X, Ding Y (2009) Comparison of yield components and plant type characteristics of high-yield rice between Taoyuan, a ‘special eco-site’ and Nanjing, China. Field Crops Res 112:214–221
Li Z, Pinson SRM, Stansel JW, Paterson AH (1998) Genetic dissection of the source-sink relationship affecting fecundity and yield in rice (Oryza sativa L.). Mol Breed 4:419–426
Ma X-F, Hua Z-T, Hao X-B, Shen F (2008) Genetic analysis on plant type of northern japonica hybrid rice. Agric Sci China 7:1281–1289
Mei HW, Luo LJ, Ying CS, Wang YP, Yu XQ, Guo LB, Paterson AH, Li ZK (2003) Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two testcross populations. Theor Appl Genet 107:89–101
Monyo JH, Whittington WJ (1973) Genotypic differences in flag leaf area and their contribution to grain yield in wheat. Euphytica 22:600–606
SAS Institute (1999) SAS/STAT user’s guide. SAS Institute, Cary
Shen B, Zhuang J-Y, Zhang K-Q, Xia Q-Q, Sheng C-X, Zheng K-L (2003) QTLs mapping of leaf traits and root vitality in a recombinant inbred line population of rice (in Chinese with English abstract). Acta Genet Sinica 30:1133–1139
Shen B, Yu W-D, Du J-H, Fan Y-Y, Wu J-R, Zhuang J-Y (2011) Validation and dissection of quantitative trait loci for leaf traits in interval RM4923-RM402 on the short arm of rice chromosome 6. J Genet 2011(90):39–44
Tong H-H, Mei H-W, Xing Y-Z, Cao Y-P, Yu X-Q, Zhang S-Q, Luo L-J (2007) QTL analysis for morphological and physiological characteristics of flag leaf at the late developmental stage in rice (in Chinese with English abstract). Chinese J Rice Sci 21:493–499
Tong H-H, Chen L, Li W-P, Mei H-W, Xing Y-Z, Yu X-Q, Xu X-Y, Zhang S-Q, Luo L-J (2010) Identification and characterization of quantitative trait loci for grain yield and its components under different nitrogen fertilization levels in rice (Oryza sativa L.). Mol Breed. doi 10.1007/s11032-010-9499-9
Wang YP, Zeng JP, Guo LB, Xing YZ, Xu CG, Mei HW, Ying CS, Luo LJ (2005) QTL and correlation analysis on characters of top three leaves and panicle weight in rice (in Chinese with English abstract). Chinese J Rice Sci 19:13–20
Yu SB, Li JX, Tan YF, Gao YJ, Li XH, Zhang QF, Saghai Maroof MA (1997) Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Proc Natl Acad Sci USA 94:9226–9231
Yuan LP (1997) Hybrid rice breeding for super high yield (in Chinese and English). Hybrid Rice 12:1–6
Yue B, Xue W-Y, Luo L-J, Xing Y-Z (2006) QTL analysis for flag leaf characteristics and their relationships with yield and yield traits in rice (in English with Chinese abstract). Acta Genet Sinica 33:824–832
Zheng KL, Huang N, Bennett J, Khush GS (1995) PCR-based marker-assisted selection in rice breeding: IRRI Discussion Paper Series no.12. International Rice Research Institute, Los Banos
Zhuang J-Y, Fan Y-Y, Rao Z-M, Wu J-L, Xia Y-W, Zheng K-L (2002) Analysis on additive effects and additive-by-additive epistatic effects of QTLs for yield traits in a recombinant inbred line population of rice. Theor Appl Genet 105:1137–1145
Zou GH, Mei HW, Liu HY, Liu GL, Hu SP, Yu XQ, Li MS, Wu JH, Luo LJ (2005) Grain yield responses to moisture regimes in a rice population: association among traits and genetic markers. Theor Appl Genet 112:106–113
Acknowledgments
This work was supported by the National Key Technologies Research and Development Program (Nr. 2011BAD35B02), the Chinese Super Rice Breeding Program (Nr. 201006), and the State Key Laboratory of Rice Biology (Nr. 080103).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shen, B., Yu, WD., Zhu, YJ. et al. Fine mapping of a major quantitative trait locus, qFLL6.2, controlling flag leaf length and yield traits in rice (Oryza sativa L.). Euphytica 184, 57–64 (2012). https://doi.org/10.1007/s10681-011-0539-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-011-0539-2