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Fine mapping of the quantitative trait locus qFLL9 controlling flag leaf length in rice

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Abstract

The morphological traits of leaves, such as size and shape, are major determinants of plant architecture and strongly affect high yield performance. To understand the molecular mechanism governing flag leaf length, we analyzed quantitative trait loci (QTLs) affecting flag leaf length by employing 176 F2 individuals derived from a cross between two japonica rice cultivars: Shennong265 (SN265) and Lijiangxintuanheigu (LTH). We identified qFLL3, qFLL6 and qFLL9 from this F2 population. Flag leaf length was increased by SN265 alleles at qFLL3 and qFLL6, but by LTH allele at qFLL9. In order to eliminate the influence of qFLL3 and qFLL6, one single residual heterozygous plant for qFLL9 region, RH-qFLL9, was selected based on the genotypes of 114 simple sequence repeat (SSR) markers and used as the parent of a segregating population. Using this segregating population of 889 plants, this region was narrowed down to an interval between RM24423 and RM24434. According to the rice annotation project database, there are 17 predicted genes in the 198-kb target region.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (30871468) and the National 973 Program of China (2009CB126007).

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Authors and Affiliations

  1. Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Northern Japonica Rice Breeding of Liaoning Province, Shenyang Agricultural University, 110866, Shenyang, China

    Shukun Jiang, Jiayu Wang, Wenfu Chen & Zhengjin Xu

  2. Dandong Academy of Agricultural Sciences, Dandong, 118109, China

    Xijuan Zhang

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  1. Shukun Jiang
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  2. Xijuan Zhang
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  3. Jiayu Wang
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  4. Wenfu Chen
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  5. Zhengjin Xu
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Correspondence to Zhengjin Xu.

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Jiang, S., Zhang, X., Wang, J. et al. Fine mapping of the quantitative trait locus qFLL9 controlling flag leaf length in rice. Euphytica 176, 341–347 (2010). https://doi.org/10.1007/s10681-010-0209-9

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  • DOI: https://doi.org/10.1007/s10681-010-0209-9

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