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Mapping of QTL for resistance to first and second generation of European corn borer using an integrated SNP and SSR linkage map

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Abstract

The European corn borer (ECB, Ostrinia nubilalis Hübner) is a major pest of maize (Zea mays L.) in North America and Europe. In this study, we mapped and characterized QTL affecting the resistance to the first (ECB1) and second generation (ECB2) of ECB and plant height with the aid of 164 SNP and 88 SSR markers. A total of 144 testcross (TC) progenies of double haploid (DH) lines developed from a cross of two parental lines from the Stiff Stalk germplasm pool were tested at six different locations in the USA under both natural and artificial infestation with ECB larvae. Resistance to ECB1 and ECB2 was assessed by measuring leaf feeding and stalk breakage, respectively, using a visual rating scale from 1 (severe damage) to 9 (no damage). Genotypic variance among the TC progenies was significant for all traits. Heritabilities were moderately high (0.69) for stalk breakage and plant height (0.75), but only moderate for leaf feeding (0.43). For stalk breakage, three QTL were detected that together explained 36% of the genetic variance, whereas for leaf feeding only one QTL was mapped, which explained 25% of the genotypic variance. For plant height, two QTL were identified, explaining 20% of the genotypic variance. The QTL for leaf feeding and stalk breakage mapped by us were located in chromosomal regions adjacent to those reported for other maize germplasm and therefore might be suitable candidates for marker-assisted selection (MAS) during line development.

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References

  • Abel CA, Berhow MA, Wilson RL, Binder BF, Hibbard BE (2000) Evaluation of conventional resistance to European corn borer (Lepidoptera: Crambidae) and Western corn rootworm (Coleoptera: Chrysomelidae) in experimental maize lines developed from a backcross breeding program. J Econ Entomol 93:1814–1821

    Article  PubMed  CAS  Google Scholar 

  • Beavis WD, Smith OS, Grant D, Fincher R (1994) Identification of quantitative trait loci using a small sample of top crossed and F4 progeny from maize. Crop Sci 34:882–896

    Article  Google Scholar 

  • Bernardo R (2002) Breeding for quantitative traits in plants. Stemma Press, Woodbury

    Google Scholar 

  • Bernardo R (2009) Should maize doubled haploids be induced among F1 or F2 plants? Theor Appl Genet 119:255–262

    Article  PubMed  CAS  Google Scholar 

  • Bohn M, Khairallah MM, Gonzàlez-De-Leòn D, Hoisington DA, Utz HF, Deutsch JA, Jewell DC, Mihn JA, Melchinger AE (1996) QTL mapping in tropical maize: I. Genomic regions affecting leaf feeding resistance to sugarcane borer and other traits. Crop Sci 36:1352–1361

    Article  Google Scholar 

  • Bohn M, Schulz B, Kreps R, Klein D, Melchinger AE (2000) QTL mapping for resistance against the European corn borer (Ostrinia nubilalis H.) in early maturing European dent germplasm. Theor Appl Genet 101:907–917

    Article  CAS  Google Scholar 

  • Brooks TD, Bushman BS, Williams WP, Mcmullen MD, Buckley PM (2007) Genetic basis of resistance to fall armyworm (Lepidoptera: Noctuidae) and southwestern corn borer (Lepidoptera: Crambidae) leaf-feeding damage in maize. J Econ Entomol 100:1470–1475

    Article  PubMed  CAS  Google Scholar 

  • Burnhamp P, Anderson DR (2004) Mutimodel inference understanding AIC And BIC in model selection. Sociol Methods Res 33:261–304

    Article  Google Scholar 

  • Cardinal AJ, Lee M (2005) Genetic relationships between resistance to stalk-tunneling by the European corn borer and cell-wall components in maize population B73×B52. Theor Appl Genet 111:1–7

    Article  PubMed  CAS  Google Scholar 

  • Cardinal AJ, Lee M, Sharopova N, Woodman-Clikeman WL, Long MJ (2001) Genetic mapping and analysis of quantitative trait loci for resistance to stalk tunneling by the European corn borer in maize. Crop Sci 41:835–845

    Article  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Fan JB, Oliphant A, Shen R, Kermani BG, Garcia F, Gunderson KL, Hansen M, Steemers F, Butler SL, Deloukas P, Galver L, Hunt S, McBride C, Bibikova M, Rubano T, Chen J, Wickham E, Doucet D, Chang W, Campbell D, Zhang B, Kruglyak S, Bentley D, Haas J, Rigault P, Zhou L, Stuelpnagel J, Chee MS (2003) Highly parallel SNP genotyping. Cold Spring Harb Symp Quant Biol 68:69–78

    Article  PubMed  CAS  Google Scholar 

  • Gardiner J, Coe E, Melia-Hancock S, Hoisington D, Chao S (1993) Development of a core RFLP map in maize using an immortalized F2 population. Genetics 134:917–930

    PubMed  CAS  Google Scholar 

  • Gould F (1998) Sustainability of transgenic insecticidal cultivars: integrating pest genetics and ecology. Annu Rev Entomol 43:701–726

    Article  PubMed  CAS  Google Scholar 

  • Groh S, Gonzàlez-De-Leòn D, Khairallah MM, Jiang C, Bergvinson D, Bohn M, Hoisington DA, Melchinger AE (1998) QTL mapping in tropical maize: III Genomics regions for resistance to Diatrea spp. and associated traits in two RIL populations. Crop Sci 38:1062–1072

    Article  Google Scholar 

  • Guthrie WD, Dicke DD, Neiswander CR (1960) Leaf and sheath feeding resistance to the European corn borer in eight inbred lines of dent corn. Ohio Agric Exp Stn Bull 860:1–38

    Google Scholar 

  • Haldane JBS (1919) The combination of linkage values and the calculation of distance between the loci of linked factors. J Genet 8:299–309

    Article  Google Scholar 

  • Haley CS, Knott SA (1992) A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69:315–324

    Article  PubMed  CAS  Google Scholar 

  • Hallauer AR, Miranda FJB (1981) Quantitative genetics in maize breeding. Iowa State Univ, Press, Ames

    Google Scholar 

  • Holm S (1979) A simple sequentially rejective multiple test procedure. Scand Stat Theory Appl 6:65–70

    Google Scholar 

  • Hutchison W, Burkness E, Mitchell P, Moon R, Leslie T, Fleischer S, Abrahamson M, Hamilton K, Steffey K, Gray M (2010) Areawide suppression of European corn borer with Bt maize reaps savings to non-Bt maize growers. Science 330:222–225

    Article  PubMed  CAS  Google Scholar 

  • James C (2009) Global status of commercialized biotech/GM crops: 2009, ISAAA briefs no. 41. International Service for the acquisition of agri-biotech application, Ithaca

    Google Scholar 

  • Jampatong C, Mcmullen MD, Barry BD, Darrah LL, Byrne PF, Kross H (2002) Quantitative trait loci for first- and second-generation European corn borer resistance derived from the maize inbred Mo47. Crop Sci 42:584–593

    Article  CAS  Google Scholar 

  • Krakowsky MD, Brinkman MJ, Woodman-Clikeman WL, Lee M (2002) Genetic components of resistance to stalk tunneling by the European corn borer in maize. Crop Sci 42:1309–1315

    Article  Google Scholar 

  • Krakowsky MD, Lee M, Woodman-Clikeman WL, Long MJ, Sharpova N (2004) QTL mapping of resistance to stalk tunneling by the European corn borer in RILs of maize population B73 × De811. Crop Sci 44:274–282

    Article  CAS  Google Scholar 

  • Lande R, Thomson R (1990) Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics 124:743–756

    PubMed  CAS  Google Scholar 

  • Lewis P et al. (2001) Bt Plant-pesticides risk and benefits assessments. SAP Report No. 2000–07a, US Environmental Protection Agency, 12 March 2001 pp. 5–13

  • Mode CJ, Robinson HF (1959) Pleiotropism and the genetic variance and covariance. Biometrics 15:518–537

    Article  Google Scholar 

  • Ordas B, Malvar RA, Santiago R, Sandoya G, Romay MC, Butron A (2009) Mapping of QTL for resistance to the Mediterranean corn borer attack using the intermated B73 × Mo17 (IBM) population of maize. Theor Appl Genet 119:1451–1459

    Article  PubMed  CAS  Google Scholar 

  • Papst C, Melchinger AE, Eder J, Schulz B, Klein D, Bohn M (2001) QTL mapping for resistance to European corn borer (Ostrinia Nubilalis Hb.) in early maturing European dent maize (Zea mays L.) germplasm and comparison of genomic regions for resistance across two populations of F3 families. Maydica 46:195–205

    Google Scholar 

  • Papst C, Bohn M, Utz HF, Melchinger AE, Klein D, Eder J (2004) QTL mapping for European corn borer resistance (Ostrinia nubilalis Hb.), agronomic and forage quality traits of testcross progenies in early-maturing European maize (Zea mays L.) germplasm. Theor Appl Genet 108:1545–1554

    Article  PubMed  CAS  Google Scholar 

  • Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics 127:181–197

    PubMed  CAS  Google Scholar 

  • Schön CC, Lee M, Melchinger AE, Guthrie WD, Woodman WL (1993) Mapping and characterization of quantitative trait loci affecting resistance against second-generation European corn borer in maize with the aid of RFLPs. Heredity 70:648–659

    Article  Google Scholar 

  • Schön CC, Utz HF, Groh S, Truberg B, Openshaw S, Melchinger AE (2004) Quantitative trait locus mapping based on resampling in a vast maize testcross experiment and its relevance to quantitative genetics for complex traits. Genetics 167:485–498

    Article  PubMed  Google Scholar 

  • Shapiro SS, Wilk MB (1965) An analysis of variance test for normality (complete samples). Biometrika 52:591–611

    Google Scholar 

  • Tabashnik BE (2008) Delaying insect resistance to transgenic crops. PNAS 105:19029–19030

    Article  PubMed  CAS  Google Scholar 

  • Utz HF (2000) PLABSTAT: a computer program for statistical analysis of plant breeding experiments. Institute for Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Stuttgart

    Google Scholar 

  • Utz HF, Melchinger AE (1996) PLABQTL–a computer program to map QTLs. Institute for Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Stuttgart

    Google Scholar 

  • Utz HF, Melchinger AE, Schön CC (2000) Bias and sampling error of the estimated proportion of genotypic variance explained by quantitative trait loci determined from experimental data in maize using cross validation and validation with independent samples. Genetics 154:1839–1849

    PubMed  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2006) JoinMap Version 4.0. Software for the calculation of genetic linkage maps in experimental populations. Plant Research International, Wageningen

    Google Scholar 

  • Willcox MC, Khairallah MM, Bergvinson D, Crossa J, Deutsch JA, Edmeades GO, González-de-León D, Jiang C, Jewell DC, Mihm JA, Williams WP, Hoisington D (2002) Selection for resistance to southwestern corn borer using marker-assisted and conventional backcrossing. Crop Sci 42:1516–1528

    Article  Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    PubMed  CAS  Google Scholar 

  • Zhao JZ, Cao J, Collins HL, Bates SL, Roush RT, Earle ED, Shelton AM (2005) Concurrent use of transgenic plants expressing a single and two Bacillus thuringiensis genes speeds insect adaptation to pyramided plants. PNAS 102:8426–8430

    Article  PubMed  CAS  Google Scholar 

  • Zhu S, Rossnagel BG, Kaeppler HF (2004) Genetic analysis of quantitative trait loci for groat protein and oil content in oat. Crop Sci 44:254–260

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to Prof. HF Utz for the insightful suggestions and to Prof. BS Dhillon for critically reviewing this manuscript.

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Authors and Affiliations

  1. Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Fruwirthstr. 21, 70599, Stuttgart, Germany

    E. Orsini, L. M. Krchov & A. E. Melchinger

  2. AgReliant Genetics, LLC, 1122 East 169th Street, Westfield, IN, 46074, USA

    J. Uphaus

Authors
  1. E. Orsini
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  2. L. M. Krchov
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  3. J. Uphaus
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  4. A. E. Melchinger
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Correspondence to E. Orsini.

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Orsini, E., Krchov, L.M., Uphaus, J. et al. Mapping of QTL for resistance to first and second generation of European corn borer using an integrated SNP and SSR linkage map. Euphytica 183, 197–206 (2012). https://doi.org/10.1007/s10681-011-0441-y

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