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Molecular tagging of a stripe rust resistance gene in Aegilops tauschii

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most important diseases of common wheat (Triticum aestivum L.). China has the largest stripe rust epidemic areas in the world and yield losses can be large. Aegilops tauschii Coss, the D-genome progenitor of common wheat, includes two subspecies, tauschii and strangulata (Eig) Tzvel. The ssp. strangulata accession AS2388 is highly resistant to the prevailing physiological races of PST in China, and possesses a single dominant gene for stripe rust resistance. In order to tag this gene, AS2388 was crossed with the highly susceptible ssp. tauschii accession AS87. The parents, F2 plants, and F2:3 families were tested at adult plant stage in field trials with six currently prevailing races. Simple sequence repeat (SSR) primers were used to identify molecular markers linked to the resistance gene. SSR markers Xwmc285 and Xwmc617 were linked to the resistance gene on chromosome arm 4DS flanking it at 1.7 and 34.6 cM, respectively. Based on the chromosomal location, this gene temporarily designated as YrAS2388 is probably novel. The resistance in Ae. tauschii AS2388 was partially expressed in two newly developed synthetic hexaploid backgrounds.

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Acknowledgments

This study was supported by National Natural Science Foundation of China (31071418), the 973 Program (2009CB118300), the 100-Talent Program of CAS, and by the Science and Technology Commission of Sichuan Province. We thank Professor Robert A McIntosh, at University of Sydney, for reviewing this manuscript.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Lin Huang, Lian-Quan Zhang, Ze-Hong Yan, You-Liang Zheng & Deng-Cai Liu

  2. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, People’s Republic of China

    Bao-Long Liu, Bo Zhang, Huai-Gang Zhang & Deng-Cai Liu

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  1. Lin Huang
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  2. Lian-Quan Zhang
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  6. Huai-Gang Zhang
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Correspondence to Huai-Gang Zhang or Deng-Cai Liu.

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Huang, L., Zhang, LQ., Liu, BL. et al. Molecular tagging of a stripe rust resistance gene in Aegilops tauschii . Euphytica 179, 313–318 (2011). https://doi.org/10.1007/s10681-010-0330-9

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  • DOI: https://doi.org/10.1007/s10681-010-0330-9

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