Abstract
Hordeum chilense Roem. et Schult. is a diploid wild South American barley that contains genes of interest for cereal breeding, many of them located on chromosome 1Hch. In the current study, two H. chilense-wheat addition lines with deletions in the 1Hch chromosome were used for sub-arm localization of five prolamin (glutenin and gliadin) loci and 33 EST-SSR marker loci on chromosome 1Hch. The two sets of markers were distributed across five sub-arm chromosome regions. Three glutenin loci (Glu-Hch2, Glu-Hch3, Glu-Hch4) together with the gliadin locus Gli-Hch1 were located on the distal 20% of the 1HchS arm, whereas the glutenin locus Glu-Hch1 was on the proximal 88% region of 1HchL. Among 33 EST-SSR marker loci, 7 (21.2%) were on the 1HchS arm and, of them, 3 (9.1%) were on the distal 20% end and 4 (12.1%) on the proximal 80% region. The 26 loci (78.8%) on 1HchL were distributed across three different regions: 18 (78.8%) in the proximal 88%, 3 (9.1%) in the distal 12% and 5 (15.2%) in a region less than 12% from the distal end. The deletions in the 1Hch chromosome added to the common wheat background were thus shown to be useful for determining the sub-arm location of EST-SSR and prolamin loci. This could facilitate the identification of molecular markers linked to genes of agronomic interest and the isolation of such genes for use in common wheat improvement.
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This research was supported by grants AGL2008-04358 and AGL2007-65685-C02-02 from the Spanish Ministry of Science and Innovation and the European Regional Development Fund (FEDER) from the European Union.
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Cherif-Mouaki, S., Said, M., Alvarez, J.B. et al. Sub-arm location of prolamin and EST-SSR loci on chromosome 1Hch from Hordeum chilense . Euphytica 178, 63–69 (2011). https://doi.org/10.1007/s10681-010-0268-y
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DOI: https://doi.org/10.1007/s10681-010-0268-y