Abstract
We report here the results of a unique, large-scale research project that focused on the problem of clonal selection of saffron (Crocus sativus L.). Samples of saffron populations from the five most ancient cultivation regions in Iran were obtained for the study, with ≥20,000 corms comprising the sampled populations from each region. The corms were divided onto weight groups (for example, 3.0–3.9, 4.0–4.9 g, up to the largest) and then planted in rows separately in the field with one corm per hole (pit). This strict separation of the corms and the strict planting procedure was maintained for four growing seasons, enabling a comparison of the pits with grown plants and a study of the differences between clones. A comparison of many hundreds and thousands of clones, each grown from one corm of the same weight, resulted in the identification of “superior” clones in terms of exceptionally large numbers of flowers and large (≥10 g) corms. Based on the number of flowers and number of large corms, which are the two most economically important attributes of saffron, the clones were classified as extraordinary, superior, ordinary, inferior and declining clones. The first two classifications of clones, which had the highest numbers of flowers and largest corms, have been chosen for use in a saffron breeding program aimed at developing new high-yielding cultivars of saffron. Those clones would also be very suitable for facilitating the mechanization of saffron agriculture in terms of the lifting, sorting and planting of corms, weeding, softening ground and harvesting flowers.
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References
Abdullaev FI (2002) Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativus L.). Exp Biol Med 227:20–25
Abdullaev FI (2004a) Antitumor effect of saffron (Crocus sativus L.): overview and perspectives. Acta Hortic 650:491–495
Abdullaev FI (2004b) Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev 28:426–432. doi:10.1016/j.cdp.2004.09.002
Agayev YM (1993) Some urgent problems in genetics, cytogenetics and breeding of saffron (Crocus sativus L.). In: Abstracts book of the second national symposium of saffron and medical plants, Gonabad, pp 12–13
Agayev YM (2002) New features in karyotype structure and origin of saffron Crocus sativus L. Cytol 67:245–252. doi:10.1508/cytologia.67.245
Agayev YM, Muzaferova RS, Savchenko SP (1975) Results of experiments of treatment of saffron corms in colchicine solution. Vestnik Selskokhozyaystvennoi Nauki (Moscow) 10:121–123
Agayev YM, Shakib AM, Soheilivand S, Fathi M (2007) Breeding of saffron (Crocus sativus): possibilities and problems. Acta Hortic 739:203–207
Akhund-zade IM, Muzaferova RS (1975) Studies on the effectiveness of gamma irradiation of the saffron. Radiobiol Mosc 15:319–322
Akhund-zade IM, Muzaferova RS (1976) The effect of an irradiation observable in the first, second and third years of vegetation of saffron. In: Proc Inst Genetics Selection Azerbaijan Acad Sci, vol 8. Baku, pp 149–153
Bagheri A, Vesal SR (2006) Genetics, sterility, propagation and in vitro production of secondary metabolites. In: Kafi M, Koocheki A, Rashed MH, Nassiri M (eds) Saffron (Crocus sativus). Production and processing. Science Publishers, India, pp 111–137
Bagheri A, Vessal S (2003) Saffron improvement in Iran, breakthroughs and barriers. In: Proc 3rd Natl Symp Saffron. Mashhad, pp 1–10
Brighton CA (1977) Cytology of Crocus sativus and its allies (Iridaceae). Plant Syst Evol 128:137–157. doi:10.1007/BF00984551
Chichiricco G (1984) Karyotype and meiotic behaviour of the triploid Crocus sativus L. Caryologia 37:233–239
Dhar AK, Sapru R, Rekha K (1988) Studies on saffron in Kashmir. 1. Variation in natural population and its cytological behavior. Crop Improv 15:48–52
Di Crecchio R (1960) Lo zafferano. Italia Agric 97:629–649
Ehsanzadeh P, Yadollahi AA, Maibodi AMM (2004) Productivity, growth and quality attributes of 10 Iranian saffron accessions under climatic conditions of Chahar-Mahal Bakhtiari, Central Iran. Acta Hortic 650:183–188
Fernandez JA (2004) Biology, biotechnology and biomedicine of saffron. Recent Res Dev Plant Sci 2:127–159
Fernandez JA (2007) Genetic resources of saffron and allies (Crocus spp.). Acta Hortic 739:167–185
Ghorbani M (2007) The economics of saffron in Iran. Acta Hortic 739:321–331
Grilli Caiola M, Di Somma D, Lauretti P (2001) Comparative study on pollen and pistil of Crocus sativus L. (Iridaceae) and its allied species. Ann Bot Roma 1:93–103
Kapinos GY (1965) Developmental biology of bulbous and cormous plants in Apsheron. Azerbaijan Academy of Sciences, Baku
Khan IA (2004) Induced mutagenic variability in saffron (Crocus sativus L.). Acta Hortic 650:281–283
Khan IA (2007) Development of high yielding saffron mutant. Acta Hortic 739:255–257
Mathew B (1977) Crocus sativus and its allies (Iridaceae). Plant Syst Evol 128:89–103. doi:10.1007/BF00985174
Munshi AM (1992) Genetic variability for important traits in saffron (Crocus sativus.). Crop Res Hisar 5:326–332
Munshi AM, Zargar GH (1991) Variation in natural population of saffron (Crocus sativus L.) crop in Kashmir and performance of some selected sub-population. Phytobreedon 7:62–67
Muzaferova RS (1970a) An influence of gamma rays on an ability to form corms of saffron, Crocus sativus L. In: Experimental mutagenesis in plants. Materials of scientific congress, Baku, Elm, pp. 93–96
Muzaferova RS (1970b) The first results studying of an influence of mutagenic factors on variability of saffron. In: Proc Inst Genet Selection. Azerbaijan Academy of Sciences, Baku, Elm, pp 194–203
Muzaferova RS (1973) A study of influence of gamma-rays on variability of saffron, Crocus sativus L. Author’s abstract of the thesis for defense of the degree of Candidate of Biological Sciences (PhD), Azerbaijan Academy of Sciences, Baku, pp 1–31
Nehvi FA, Wani SA, Dar SA, Makhdoomi MI, Allie BA, Mir ZA (2007a) Biological interventions for enhancing saffron productivity in Kashmir. Acta Hortic 739:25–31
Nehvi FA, Wani SA, Dar SA, Makhdoomi MI, Allie BA, Mir ZA (2007b) New emerging trends on production technology of saffron. Acta Hortic 739:375–381
Picci V (1987) A summary of experiments on the cultivation of Crocus sativus L. in Italy. Atti Convegno sulla Coltivazione delle Piante Officinali, Ministero dell’Agricoltura e delle Foreste, Italy, pp 119–157
Piccioli G (1932) La coltura dello zafferano ne L’Aquila degli Abruzzi. Cellamare, L’Aquila
Piqueras A, Han BH, Escribano J, Rubio C, Hellin E, Fernandez JA (1999) Development of cormogenic nodules and microcorms by tissue culture, a new tool for the multiplication and genetic improvement of saffron. Agronomy 19:603–610. doi:10.1051/agro:19990704
Rzakuliyev IM (1948) Flowering biology of saffron (Crocus sativus L.). Izvestiya AN Azerb. SSR (Baku) 2:105–114
Rzakuliyev IM (1959) A study of different specimens of saffron in condition of Apsheron. AGU, Uchyoniye Zapiski, Biological series, no. 5, pp 3–8
Singh P (2001) Essentials of plant breeding. Kalyani Publ, New Delhi
Tammaro F (1987) Notizie storico-colturali sullo zafferano (Crocus sativus L. Iridaceae) nell’area mediterranea. Micol Veget Medit 2:44–59
Vurdu H (2004) Room table: agronomical and biotechnological approaches for saffron improvement. Acta Hortic 650:285–290
Zaffar G, Wani SA, Anjum T, Zeerak NA (2004) Colchicine induced variability in saffron. Acta Hortic 650:277–280
Zargar GH (2002) Genetic variation in saffron and importance of quality seed corms. National seminar-cum-workshop, Srinagar
Acknowledgments
This work was funded by the Agricultural Biotechnology Research Institute of Iran (ABRII) (Project number: 1-100-14-00-00-03-83-03-00-00). The authors thank Dr. B. Ghareyazie for his assistance in the acceptance and performance of the project, respected reviewers for their valuable comments and discussions on the manuscript and the agricultural assistant M. Fathi for his irreproachable help.
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Agayev, Y.Mo., Fernandez, JA. & Zarifi, E. Clonal selection of saffron (Crocus sativus L.): the first optimistic experimental results. Euphytica 169, 81–99 (2009). https://doi.org/10.1007/s10681-009-9946-z
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DOI: https://doi.org/10.1007/s10681-009-9946-z