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Molecular marker diversity and bacterial wilt resistance in wild Solanum commersonii accessions from Uruguay

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Abstract

Solanum commersonii is a wild tuber-bearing species native to Uruguay with high potential for use in potato breeding programs. Little is known about the genetic diversity within this wild species and the relationship with the resistance to the bacterial pathogen Ralstonia solanacearum. We studied 30 S. commersonii clonal accessions, 20 of which were collected from geographically different areas across the country, while the other ten were grown from seeds from a single plant. Resistance against R. solanacearum was tested and different levels of resistance were found, ranging from delayed wilting to asymptomatic reactions. The genetic variation and the relationships among individuals in this germplasm collection were studied by different molecular markers: Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Microsatellites or Simple Sequence Repeats (SSR). AFLP markers generated the largest number of total and polymorphic fragments per assay unit while SSR revealed the highest frequency of polymorphic bands (100%), followed by AFLP (96.2%) and RAPD (89.4%). In contrast, when comparing the number of different genetic profiles generated, the SSR markers exhibited the lowest discriminatory power. The clustering pattern obtained with the three marker systems showed a similar distribution of the S. commersonii germplasm revealing a high correlation between the three methods employed. All three dendrograms grouped most of the accessions into two main clusters, containing the same accessions regardless of the marker type. Bacterial wilt resistant accessions were present in both clusters. Accessions originated from different seeds of the same plant were grouped within one of the major clusters, and differed in the response to R. solanacearum revealing segregation of resistance. Furthermore, the distribution in two main clusters showed high correspondence with the geographical origin of the accessions, from the north and south of the country, and with the subspecies malmeanum and commersonii morphologically identified.

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Acknowledgments

We thank Dr. Patrick Hinrichsen at the Laboratorio de Biotecnología, INIA La Platina, Chile, for kindly allowing Mrs. Siri to perform the AFLP and SSR techniques. This research was supported in part by the Program for the Development of Basic Sciences (PEDECIBA, MSc fellowship) and the Technologic Development Program in Uruguay (PDT 32–24). We thank Dr. Valerie Dee for linguistic revision of this paper.

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Authors and Affiliations

  1. Departamento de Biociencias, Facultad de Química, Universidad de la República, Gral. Flores 2124, Montevideo, Uruguay

    M. I. Siri, L. Franco Fraguas & M. J. Pianzzola

  2. Departamento de Producción y Protección Vegetal, Facultad de Agronomía, Universidad de la República, Av. Garzón 780, Montevideo, Uruguay

    G. A. Galván, L. Quirici & E. Silvera

  3. Departamento de Química Orgánica, Facultad de Química, Universidad de la República, Gral. Flores 2124, Montevideo, Uruguay

    P. Villanueva & F. Ferreira

Authors
  1. M. I. Siri
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  2. G. A. Galván
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  3. L. Quirici
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  4. E. Silvera
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  5. P. Villanueva
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  6. F. Ferreira
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  7. L. Franco Fraguas
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  8. M. J. Pianzzola
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Correspondence to M. J. Pianzzola.

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Siri, M.I., Galván, G.A., Quirici, L. et al. Molecular marker diversity and bacterial wilt resistance in wild Solanum commersonii accessions from Uruguay. Euphytica 165, 371–382 (2009). https://doi.org/10.1007/s10681-008-9800-8

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  • DOI: https://doi.org/10.1007/s10681-008-9800-8

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