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Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer

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Transgenic Korean rice plants containing the cry1Ab gene were developed for resistance against yellow stem borer (Scirpophaga incertulas, YSB). More than 100 independent transgenic lines from three Korean varieties (P-I, P-II and P-III) were generated. The amount of Cry1Ab in transgenic T0 plants was as high as 2.88% of total soluble proteins. These levels were sufficient to cause 100% mortality of YSB larvae. The majority of T1 transgenic lines originated from the varieties P-I and P-II followed a Mendelian fashion of segregation. Deviation from the expected segregation ratio was observed in a small number of the transgenic lines of P-I and P-II origins. However, this deviation was primarily observed in the P-III originated lines. Segregation analysis of the T1 generation indicated that 1–3 copies of the cry1Ab gene were integrated into the genome of the majority of the transgenic lines originating from varieties P-I and P-II. Stunted and semi-fertile mutants were observed in some transgenic lines. These aberrations were either independent or closely linked to the introduced cry1Ab gene loci in different transgenic lines. Reduction in GUS expression levels and loss of toxicity against YSB larvae were found in some transgenic lines. The transgenic T3 and T4 lines causing 100% mortality of third instar YSB larvae in the lab were completely protected in the field. Analysis of important yield components on nine selected transgenic lines indicated that stem length, panicle length, grain number per panicle, and seed setting rates were reduced in transgenic plants compared to those in non-transgenic parental rice lines. Number of panicles per cluster, however, was significantly higher in transgenic plants. The numerical value of the average yield was in general greater in the controls than in all the transgenic lines, indicating some ‘yield drag’. Since some selected lines were highly resistant to the YSB with good yielding potential, they offer effective potential for use in insect resistance management programs.

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Acknowledgements

This work was funded by grants from The Rockefeller Foundation and Natural Sciences and Engineering Research Council of Canada to IA. We are thankful to Dr Jefferson, CAMBIA, Australia for kindly providing the plant expression vector pCAMBIA1305.1. We are also grateful to Dr Upadhyaya, CSIRO, Australia for his scientific advice.

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Authors and Affiliations

  1. Biotechnology Laboratory, Research Institute of Agrobiology, Academy of Agricultural Sciences, Pyongyang, Democratic People’s Republic of Korea

    Songjin Kim, Choljun Kim, Wonnam Li & Tokyong Kim

  2. Biology Faculty, Pyongyang University of Agriculture, Pyongyang, Democratic People’s Republic of Korea

    Yongsu Li

  3. Agricultural Biotechnology Laboratory, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada, K1H 8M5

    Mohsin Abbas Zaidi & Illimar Altosaar

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  1. Songjin Kim
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Correspondence to Illimar Altosaar.

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Kim, S., Kim, C., Li, W. et al. Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer. Euphytica 164, 829–839 (2008). https://doi.org/10.1007/s10681-008-9739-9

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