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Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars

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Abstract

The S-genotypes of 16 apricot (Prunus armeniaca L.) cultivars native to China were determined by the S-allele PCR approach and the results were confirmed by cross-pollination tests among these cultivars. Primer combination EM-PC2consFD + EM-PC3consR, based on the conserved regions C2 and C3 of Rosaceous S-RNase genes, was the most useful primer combination for identifying Chinese apricot S-alleles. Twelve S-RNase alleles were identified using this primer combination, and they were defined as follows: S 9 was 657 bp, S 10 was 266 bp, S 11 was 464 bp, S 12 was 360 bp, S 13 was 401 bp, S 14 was 492 bp, S 15 was 469 bp, S 16 was 481 bp, S 17 was 487 bp, S 18 was 1337 bp, S 19 was 546 bp and S 20 was 1934 bp. S 11–S 20 were new S-RNase genes deposited in GenBank under accession numbers DQ868316, DQ870628-DQ870634, EF133689 and EF160078, respectively. Our findings contribute to a more efficient breeding program of Chinese apricot and further studies on the S-RNase genes.

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Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (No. 30370992). Authors want to thank Dr. Adam J Lukaszewski (University of California) for his help in revision of this manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China

    Lijie Zhang, Xuesen Chen, Xiaoliu Chen, Chunyu Zhang, Xiaoli Liu, Zhijuan Ci, Hong Zhang, Chuanjin Wu & Chongqi Liu

  2. Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, 350013, P.R. China

    Lijie Zhang

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  1. Lijie Zhang
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  2. Xuesen Chen
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  3. Xiaoliu Chen
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  4. Chunyu Zhang
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  5. Xiaoli Liu
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  7. Hong Zhang
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Correspondence to Xuesen Chen.

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Zhang, L., Chen, X., Chen, X. et al. Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars. Euphytica 160, 241–248 (2008). https://doi.org/10.1007/s10681-007-9544-x

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  • DOI: https://doi.org/10.1007/s10681-007-9544-x

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