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Analysis of hybrid lethality in F1 wheat-rye hybrid embryos

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Abstract

The effect of the Embryo lethality mutant (Eml) of rye was studied in crosses between hexaploid wheat and corresponding inbred rye line (L2). Histological analysis of hybrid embryos revealed morphological differences 16 days after pollination. Eml was found to arrest the formation of shoot meristem but had no influence on root meristem formation. The effect of Eml cannot be overcome by in vitro embryo rescue via direct regeneration on Kruse medium. The possibility of complementary interactions between wheat and rye genes and of changes in gene expression through increased variation in dosage-regulated gene expression during hybrid formation is discussed.

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References

  • Baum M, Lagudah ES, Appels R (1992) Wide crosses in cereals. Annu Plant Physiol Plant Mol Biol 43:117–143

    Article  Google Scholar 

  • Burke JM, Arnold Ml (2001) Genetics and fitness of hybrids. Ann Rev Genet 35:31–52

    Article  PubMed  CAS  Google Scholar 

  • Clark JK (1996) Maize embryogenesis mutants. In: Wang TL, Cuming A (eds) Embryogenesis: the generation of a plant. Bios Scientific Publishers, Oxford, pp 89–112

    Google Scholar 

  • Clark JK, Sheridan WF (1986) Developmental profiles of the maize embryo-lethal mutants dek22 and dek23. J Hered 77:83–92

    Google Scholar 

  • Clark JK, Sheridan WF (1988) Characterization of the two maize embryo-lethal defective kernel mutants rgh*-1210 and fl*-1253B: Effects on embryo and gametophyte development. Genetics 120:179–290

    Google Scholar 

  • Comai L, Tyagi AP, Winter K, Holmes-Davis R, Reynolds SH, Stevens Y, Byers B (2000) Phenotypic instability and rapid gene silencing in newly formed Arabidopsis allotetraploids. Plant Cell 12:1551–1567

    Article  PubMed  CAS  Google Scholar 

  • Dobzhansky TH (1937). Genetics and the origin of species. Columbia University Press, New York

    Google Scholar 

  • Feldman M, Liu B, Segal G, Abbo S, Levy AA, Vega JM (1997) Rapid elimination of low-copy DNA sequences in polyploid wheat: possible mechanism for differentiation of homeologous chromosomes. Genetics 147:1381–1387

    PubMed  CAS  Google Scholar 

  • Gernand D, Rutten T, Varshney A, Rubtsova M, Prodanovic S, Brüß C, Kumlehn J, Matzk F, Houben A (2005) Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation. Plant Cell 17:2431–2438

    Article  PubMed  CAS  Google Scholar 

  • Gerstel DU (1954) A new lethal combination in interspecific cotton hybrids. Genetics 39:628–639

    PubMed  CAS  Google Scholar 

  • Gerstel DU, Burns JA, Burk LG (1979) Interspecific hybridizations with an African tobacco, Nicotiana africana Merxm. J Hered 70:342–344

    Google Scholar 

  • Hermsen JGTh (1963) The genetic basis of hybrid necrosis in wheat. Genetica 33:445–487

    Article  Google Scholar 

  • Hermsen JGTh (1967) Hybrid dwarfness in wheat. Euphytica 16:134–162

    Article  Google Scholar 

  • Hollingshead L (1930) A lethal factor in Crepis effective only in an interspecific hybrid. Genetics 15:114–140

    PubMed  CAS  Google Scholar 

  • Inoue E, Marubashi W, Niwa M (1994) Simple method for overcoming the lethality observed in the hybrid between Nicotiana suaveolens and N. tabacum. Breed Sci 44:333–336

    Google Scholar 

  • Inoue E, Marubashi W, Niwa M (1996) Genomic factor controlling the lethality exhibited in the hybrid between Nicotiana suaveolens Lehm. and N. tabacum L. Theor Appl Genet 93:341–347

    Article  CAS  Google Scholar 

  • Inoue E, Marubashi W, Niwa M (1997) Improvement of the method for overcoming the hybrid lethality between Nicotiana suaveolens and N. tabacum by culture of F1 seeds in liquid media containing cytokinins. Breed Sci 47:221–216

    Google Scholar 

  • Inoue E, Marubashi W, Niwa M (2000) Characterization and overcoming of temperature-dependent lethality exhibited by hybrid embryos from the cross Nicotiana suaveolens × N. sylvestris. Breed Sci 50:297–302

    CAS  Google Scholar 

  • Itoh J, Sato Y, Nagato Y, Matsuoka M (2006) Formation, maintenance and function of the shoot apical meristem in rice. Plant Mol Biol 60:827–842

    Article  PubMed  CAS  Google Scholar 

  • Kaltsikes PJ (1974) Methods for triticale production. Z Pflanzenzücht 71(3):264–286

    Google Scholar 

  • Kobori S, Marubashi W (2004) Programmed cell death detected in interspecific hybrids of Nicotiana repanda × N. tomentosiformis expressing hybrid lethality. Breed Sci 54:347–350

    Article  Google Scholar 

  • Krowlow KD (1970) Untersuchungen über die Kreuzbarkeit zwischen Weizen und Roggen. Z Pflanzenzüchtg 64:44–72

    Google Scholar 

  • Kruse A (1974) An in vivo/vitro embryo culture technique. Hereditas 2:219–224

    Google Scholar 

  • Larter E, Tsuchiya T, Evans LE (1968) Breeding and cytology of triticale. Proceeding of 3rd Inter. Wheat Symp., Canberra, 1968, pp 213–221

  • Laurie DA, Bennett MD (1986) Wheat × maize hybridization. Can J Genet Cytol 28:313–316

    Google Scholar 

  • Laurie DA, Bennett MD (1988a) The production of haploid wheat plants from wheat x maize crosses. Theor Appl Genet 76:393–397

    Article  Google Scholar 

  • Laurie DA, Bennett MD (1988b) Cytological evidence for fertilization in hexaploid wheat × sorghum crosses. Plant Breed 100:73–82

    Article  Google Scholar 

  • Lee JA (1981) Genetics of D3 complementary lethality in Gossypium hirsutum and G. barbadense. J Hered 72:299–300

    Google Scholar 

  • Levin DA (1983) Polyploidy and novelity in flowering plants. Am Nat 122:1–25

    Article  Google Scholar 

  • Liu B, Vega JM, Segal G, Abbo S., Rodova M, Feldman M (1998a) Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. I. Changes in low-copy noncoding DNA sequences. Genome 41:272–277

    Article  CAS  Google Scholar 

  • Liu B, Vega JM, Feldman M (1998b) Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. II. Changes in low-copy coding DNA sequences. Genome 41:535–542

    Article  PubMed  CAS  Google Scholar 

  • Ma X-F, Fang P, Gustafson JP (2004) Polyploidization-induced genome variation in triticale. Genome 47:839–848

    Article  PubMed  CAS  Google Scholar 

  • Magnard JL, Heckel T, Massonneau A, Wisniewski JP, Cordelier S, Lassagne H, Perez P, Dumas Ch, Rogowski PM (2004) Morphogenesis of maize embryos requires ZmPRPL35-1 encoding a plastid ribosomal protein. Plant Physiol 134:649–663

    Article  PubMed  CAS  Google Scholar 

  • Marubashi W, Yamada T, Niwa M (1999) Apoptosis detected in hybrids between Nicotiana glutinosa and N. repanda expressing lethality. Planta 210:168–171

    Article  PubMed  CAS  Google Scholar 

  • Masuda Yu, Yamada T, Marubashi W (2003) Time course analysis of apoptotic cell death during expression of hybrid lethality in hybrid tobacco cells (Nicotiana suaveolens × N. tabacum) Plant Cell Physiol 44(4):420–427

    Article  PubMed  CAS  Google Scholar 

  • Matzk F, Mahn A (1994) Improved techniques for haploid production in wheat using chromosome elimination. Plant Breed 113(2):125–129

    Article  Google Scholar 

  • May CE, Appels R (1980) Rye chromosome translocation in hexaploid wheat: a re-evaluation of the loss of heterochromatin from rye chromosomes. Theor Appl Genet 56:17–23

    Article  Google Scholar 

  • May CE, Appels R (1984) Seedling lethality in wheat: a novel phenotype associated with a 2RS/2BL translocation chromosome. Theor Appl Genet 68:163–168

    Article  Google Scholar 

  • Müntzing A (1979) Triticale: Results and Problems. J Plant Breed Suppl 10:1–103

    Google Scholar 

  • Oettler G (1983) Crossability and embryo development in wheat-rye hybrids. Euphytica 32:593–600

    Article  Google Scholar 

  • Oka H (1957) Phylogenetic differentiation of cultivated rice. XV. Complementary lethal genes in rice. Jpn J Genet 32:83–87

    Google Scholar 

  • Orr HA, Presgraves DC (2000) Speciation by postzygotic isolation: forces, genes and molecules. Bioessays 22:1085–1094

    Article  PubMed  CAS  Google Scholar 

  • Osborn TC, Pires JC, Birchler JA, Auger DL, Chen ZJ, Lee HS, Comai L, Madlung A, Doerge RW, Colot V, Martienssen RA (2003) Understanding mechanisms of novel gene expression in polyploids. Trend Genet 19:141–147

    Article  CAS  Google Scholar 

  • Pilu R, Consonni G, Busti E, MacCabe AP, Giulini A, Dolfini S, Gavazzi G (2002) Mutations in two independent genes lead to suppression of the shoot apical meristem in maize. Plant Physiol 128:502–511

    Article  PubMed  CAS  Google Scholar 

  • Raina SK (1984) Crossability and in vitro development of hybrid embryos of Triticum durum × Secale cereale. Indian J Genet 44(3):429–437

    Google Scholar 

  • Rapp RA, Wendel JF (2005) Epigenetics and plant evolution. New Phytol 168:81–91

    Article  PubMed  CAS  Google Scholar 

  • Rieseberg LH, Carney SE (1998) Plant hybridisation. New Phytol 140:599–624

    Article  Google Scholar 

  • Satoh N, Hong SK, Nishimura A, Matsuoka M, Kitano H, Nagato Y (1999) Initiation of shoot apical meristem in rice: characterization of four SHOOTLESS genes. Development 126:3629–3636

    PubMed  CAS  Google Scholar 

  • Sawant AC (1956) Semilethal complementary factors in a tomato species hybrid. Evolution 10:93–96

    Article  Google Scholar 

  • Sears ER (1940) Monofactorially conditioned inviability of an intergeneric hybrid in the Triticinae. Genetics 25:133

    Google Scholar 

  • Sears ER (1944) Inviability of intergeneric hybrids involving Triticum monococcum and T. aegilopoides. Genetics 29:113–127

    PubMed  CAS  Google Scholar 

  • Shao ZZ, Taira T (1990) Production of primary triticale from calluses of immature abnormal hybrid embryos between Triticum durum and Secale cereale. Plant Breed 105:81–88

    Article  Google Scholar 

  • Sheridan WF, Neuffer MG (1981) Maize mutants altered in embryo development. In: Subtelney S, Abbott U (eds) Levels of genetic control in development. Alan R Liss, New York, pp 137–156

    Google Scholar 

  • Shii CT, Mok MC, Temple SR, Mok DWS (1980) Expression of developmental abnormalities in hybrid of Phaseolus vulgaris L. Interaction between temperature and allelic dosage. J Hered 71:218–222

    Google Scholar 

  • Sirkka A, Immonen T (1993) Comparison of callus culture with embryo culture at different times of embryo rescue for primary triticale production. Euphytica 70:185–190

    Article  Google Scholar 

  • Sitch LA, Snape JW (1987) Factors affecting haploid production in wheat using the Hordeum bulbosum system. 1. Genotypic and environmental effects on pollen germination, pollen tube growth and the frequency of fertilization. Euphytica 36:483–496

    Article  Google Scholar 

  • Smirnov VG, Sosnikhina SP (1984) Genetika rzhi (Genetics of rye). Leningr Gos Univ., 264 pp

  • Snape JW, Chapman V, Moss J, Blanchard CE, Miller TE (1979) The crossabilities of wheat varieties with Hordeum bulbosum. Heredity 42:29–298

    Google Scholar 

  • Stebbins GL (1957) The inviability, weakness and sterility of interspecific hybrids. Adv Genet 9:147–215

    Article  Google Scholar 

  • Taira T, Larter EN (1978) Factors influencing development of wheat-rye hybrid embryos in vitro. Crop Scien 18:348–350

    Article  CAS  Google Scholar 

  • Takahashi R, Hayashi J, Moriya I (1970) Studies on lethal seedling of barley by complementary genes. I. Mode of inheritance and the geographical distribution of the lethal genes. Nogaku Kenkyu 53:197–204

    Google Scholar 

  • Tezuka T, Marubashi W (2006) Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens: evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers. Theor Appl Genet 112:1172–1178

    Article  PubMed  CAS  Google Scholar 

  • Tikhenko ND, Pyukkenen VP, Voylokoy AV (2003) Specific of embryo development in hybrid wheat-rye corn seeds. Proceeding of 5th international symposium “New and nontraditional plants and prospects of their use”. Moscow 2003, vol 2, pp 441–443

  • Tikhenko ND, Tsvetkova NV, Voylokoy AV (2005) Genetic control of embryo lethality in crosses between common wheat and rye. Russ J Genet 41(8):877–884

    Article  CAS  Google Scholar 

  • Tikhenko N, Tsvetkova N, Börner A, Voylokov V (2006) Genetic study of embryo lethality in wheat-rye hybrids. In: Book of Abstracts International Symposium on Rye Breeding & Genetics, Groβ Lüsewitz, 28–30 June 2006, p 69

  • Voylokov AV, Tikhenko ND (2002) Triticale as a model for study of genome interaction and genome evolution in allopolyploid plants. Proceeding of 5th international Triticale symposium, Radzikow, 30 June–5 July 2002, vol 1, pp 63–69

  • Worland AJ, Law CN (1980) The genetics of hybrid dwarfing in wheat. Z Pflanzenzüchtg 85:28–39

    Google Scholar 

  • Yamada T, Marubashi W, Niwa M (2000) Apoptotic cell death induces temperature-sensitive lethality in hybrid seedlings and calli derived from the cross of Nicotiana suaveolens × N. tabacum. Planta 211:614–622

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Dr F. Matzk for providing the facilities for embryo rescue and tissue culture together with Dr Richard Pickering for critical reading of the manuscript. We are grateful to K. Kumke, M. Hantschmann, M. Pürschel, H. Büchner and H. Block for excellent technical assistance. This work was supported by a grant from the Deutsche Forschungsgemeinschaft and the Russian Foundation for Basic Research: project 06-04-48758a.

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Authors and Affiliations

  1. Plant Genetics Lab, Biological Institute of St. Petersburg State University, Oranienbaumskoe schosse 2, St. Petersburg, Stariy Peterhof, 198504, Russia

    N. Tikhenko & A. Voylokov

  2. SPb Branch, Vavilov Institute of General Genetics (RAS), 199034, St. Petersburg, Russia

    N. Tikhenko & A. Voylokov

  3. Leibnitz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466, Gatersleben, Germany

    T. Rutten & A. Houben

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  1. N. Tikhenko
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  2. T. Rutten
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Correspondence to N. Tikhenko.

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Tikhenko, N., Rutten, T., Voylokov, A. et al. Analysis of hybrid lethality in F1 wheat-rye hybrid embryos. Euphytica 159, 367–375 (2008). https://doi.org/10.1007/s10681-007-9528-x

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  • DOI: https://doi.org/10.1007/s10681-007-9528-x

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